Construct: sgRNA BRDN0001148036

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TAATACTCACAGGTGTAAGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FPGT-TNNI3K (100526835)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75905

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000001.11 1 74342980 - TNNI3K NNNTACTCACAGGTGTAAGG NGG 0 1.0 Tier I
2 NC_000001.11 1 74342980 - FPGT-TNNI3K NNNTACTCACAGGTGTAAGG NGG 0 1.0 Tier I
3 NC_000016.10 16 72794328 + ZFHX3 NNNTACTCACAGGTGAGAGG NGG 2 0.1604 Tier I
4 NC_000018.10 18 70010631 + RTTN NNNTGCTCACAGGTGAAAGG NGG 2 0.6545 Tier II
5 NC_000023.11 X 92431475 + PCDH11X NNNTACTCACACGTATAAGG NGG 2 0.4983 Tier II
6 NC_000024.10 Y 5544608 + PCDH11Y NNNTACTCACACGTATAAGG NGG 2 0.4983 Tier II
7 NC_000001.11 1 48589260 + AGBL4 NNNTACTCTCAGGTGTAAAG NGG 2 0.4286 Tier II
8 NC_000009.12 9 2132251 + SMARCA2 NNNTCCTCACAGGTGTAAAG NGG 2 0.3571 Tier II
9 NC_000007.14 7 108476446 - PNPLA8 NNNTCCTAACAGGTGTAAGG NGG 2 0.325 Tier II
10 NC_000006.12 6 4134202 + ECI2 NNNTACACACAGGTGGAAGG NGG 2 0.3029 Tier II
11 NC_000003.12 3 52237479 + TWF2 NNNTGCTCACAGGTGGAAGG NGG 2 0.2492 Tier II
12 NC_000006.12 6 152237174 - SYNE1 NNNTACTCACAGTTGTAAAG NGG 2 0.2143 Tier II
13 NC_000007.14 7 102424523 + PRKRIP1 NNNTGCTCACAGGTGTCAGG NGG 2 0.1271 Tier II
14 NC_000022.11 22 33586404 - LARGE1 NNNTATTCACAGGTGTAAGG NTG 2 0.0362 Tier II
15 NC_000017.11 17 66551179 - PRKCA NNNTACTCACAGGGCTAAGG NGG 2 0.0 Tier II
16 NC_000006.12 6 133587340 - TARID NNNTACTCACAGGAGAAAGG NGG 2 0.5628 Tier III
17 NC_000003.12 3 186705841 - HRGP2 NNNTACTCACAGGTGTAGGA NGG 2 0.375 Tier III
18 NC_000008.11 8 32933332 - LOC105379362 NNNTAGTCAGAGGTGTAAGG NGG 2 0.1944 Tier III
19 NC_000016.10 16 72794328 + ZFHX3-AS1 NNNTACTCACAGGTGAGAGG NGG 2 0.1604 Tier III
20 NC_000007.14 7 102424523 + LOC100630923 NNNTGCTCACAGGTGTCAGG NGG 2 0.1271 Tier III
21 NC_000001.11 1 72766652 + LINC02796 NNNTACTCACAGGTGGAAGG NAG 2 0.0897 Tier III
22 NC_000001.11 1 72766652 + LOC105378798 NNNTACTCACAGGTGGAAGG NAG 2 0.0897 Tier III
23 NC_000005.10 5 80905198 + LOC107986428 NNNTACTCACAGGTCTGAGG NGG 2 0.0481 Tier III
24 NC_000022.11 22 33586404 - LOC105373007 NNNTATTCACAGGTGTAAGG NTG 2 0.0362 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000074.6 8 24711307 + Adam3 NNNTACTCACAGGAGTAAGT NGG 2 0.4333 Tier I
2 NC_000084.6 18 63245578 + Piezo2 NNNTACTCACAGTTGTAAGC NGG 2 0.1286 Tier I
3 NC_000067.6 1 191350836 + Pacc1 NNNTACTCACAGGTATAAGG NGG 1 0.9412 Tier II
4 NC_000071.6 5 105096952 - Gbp9 NNNTACTGACAGGTGTAAGA NGG 2 0.6027 Tier II
5 NC_000069.6 3 139303907 - Stpg2 NNNTACTGACAGGTGAAAGG NGG 2 0.5844 Tier II
6 NC_000079.6 13 44868936 + Jarid2 NNNTACACACAGGAGTAAGG NGG 2 0.5417 Tier II
7 NC_000067.6 1 175139160 + Rgs7 NNNTACTCAAAGGAGTAAGG NGG 2 0.5365 Tier II
8 NC_000069.6 3 67339902 - Rsrc1 NNNTACTCACAGGTGCAAAG NGG 2 0.4762 Tier II
9 NC_000079.6 13 100085421 + Bdp1 NNNTACTCACAGGAGTAAGT NGG 2 0.4333 Tier II
10 NC_000069.6 3 29999722 - Mecom NNNAACTCAAAGGTGTAAGG NGG 2 0.4127 Tier II
11 NC_000075.6 9 86665621 - Me1 NNNAACTGACAGGTGTAAGG NGG 2 0.3061 Tier II
12 NC_000083.6 17 55991782 + Fsd1 NNNTACTCTCAGGTGTATGG NGG 2 0.3 Tier II
13 NC_000067.6 1 119753102 + Ptpn4 NNNTTCTGACAGGTGTAAGG NGG 2 0.2338 Tier II
14 NC_000068.7 2 67863268 - B3galt1 NNNTTCTGACAGGTGTAAGG NGG 2 0.2338 Tier II
15 NC_000072.6 6 70780803 - Rpia NNNTTCTGACAGGTGTAAGG NGG 2 0.2338 Tier II
16 NC_000077.6 11 108565103 + Cep112 NNNTTCTGACAGGTGTAAGG NGG 2 0.2338 Tier II
17 NC_000081.6 15 28325381 + Dnah5 NNNTTCTGACAGGTGTAAGG NGG 2 0.2338 Tier II
18 NC_000086.7 X 105986851 - Magt1 NNNTTCTGACAGGTGTAAGG NGG 2 0.2338 Tier II
19 NC_000070.6 4 104530078 + Dab1 NNNTTCTCACAGGAGTAAGG NGG 2 0.2251 Tier II
20 NC_000079.6 13 91070230 - Atg10 NNNTACTGACTGGTGTAAGG NGG 2 0.1978 Tier II
21 NC_000068.7 2 28615515 - Gfi1b NNNTATTCACAGGTGTCAGG NGG 2 0.1639 Tier II
22 NC_000072.6 6 54608829 + Plekha8 NNNTTCTCACTGGTGTAAGG NGG 2 0.1119 Tier II
23 NC_000086.7 X 96445691 + Hsf3 NNNTTCTCACTGGTGTAAGG NGG 2 0.1119 Tier II
24 NC_000080.6 14 29463447 - Cacna2d3 NNNGACTCACAGGTGTGAGG NGG 2 0.1103 Tier II
25 NC_000072.6 6 88576212 - Kbtbd12 NNNTACTCACAGGTGTTAGT NGG 2 0.0933 Tier II
26 NC_000079.6 13 119740351 - Nim1k NNNTACTCACAGGTATAAGG NGA 2 0.0654 Tier II
27 NC_000076.6 10 32080361 + Nkain2 NNNTTCTCACAGGTGTGAGG NGG 2 0.0642 Tier II
28 NC_000080.6 14 87418029 + Tdrd3 NNNTACTCACAGGTGTAAAG NTG 2 0.0278 Tier II
29 NC_000073.6 7 68316007 + Fam169b NNNTACTCCCAGGTGTAAGG NTG 2 0.0223 Tier II
30 NC_000069.6 3 18383753 + Gm30667 NNNTACTCACAGGTATAAGA NGG 2 0.8824 Tier III
31 NC_000067.6 1 74824470 - Gm32275 NNNTACTGACAGATGTAAGG NGG 2 0.5934 Tier III
32 NC_000069.6 3 29999722 - Gm52621 NNNAACTCAAAGGTGTAAGG NGG 2 0.4127 Tier III
33 NC_000072.6 6 70225563 - Igk NNNTTCTCACAGGTGTAAGA NGG 2 0.3409 Tier III
34 NC_000086.7 X 11505023 + Gm39496 NNNTACTCACATGTGTAAGT NGG 2 0.2692 Tier III
35 NC_000079.6 13 78552966 + Gm32067 NNNTTCTGACAGGTGTAAGG NGG 2 0.2338 Tier III
36 NC_000085.6 19 39940273 - Cyp2c71-ps NNNTACTGACTGGTGTAAGG NGG 2 0.1978 Tier III
37 NC_000072.6 6 60120747 - Gm5570 NNNTACTGACAGGTGTGAGG NGG 2 0.1134 Tier III
38 NC_000068.7 2 13563756 + Gm9875 NNNTTCTCACTGGTGTAAGG NGG 2 0.1119 Tier III
39 NC_000086.7 X 148058711 - Gm15110 NNNTTCTCACTGGTGTAAGG NGG 2 0.1119 Tier III
40 NC_000086.7 X 148378024 - Gm15113 NNNTTCTCACTGGTGTAAGG NGG 2 0.1119 Tier III
41 NC_000086.7 X 148546964 - Gm15112 NNNTTCTCACTGGTGTAAGG NGG 2 0.1119 Tier III
42 NC_000086.7 X 148729371 - Gm15126 NNNTTCTCACTGGTGTAAGG NGG 2 0.1119 Tier III
43 NC_000086.7 X 148949985 - Gm15094 NNNTTCTCACTGGTGTAAGG NGG 2 0.1119 Tier III
44 NC_000086.7 X 149161448 - Gm15090 NNNTTCTCACTGGTGTAAGG NGG 2 0.1119 Tier III
45 NC_000086.7 X 149330547 - Gm15089 NNNTTCTCACTGGTGTAAGG NGG 2 0.1119 Tier III
Download CSV

Other clones with same target sequence:

(none)