Construct: sgRNA BRDN0001148039
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TCAGGGGTAATGATGAGGCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ITPKC (80271)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77651
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000019.10 |
19 |
40718123 |
- |
ITPKC |
NNNGGGGTAATGATGAGGCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
203182277 |
+ |
CHI3L1 |
NNNGGGGTGAGGATGAGGCG |
NGG |
2 |
0.2667 |
Tier I |
| 3 |
NC_000001.11 |
1 |
175650732 |
- |
TNR |
NNNGGGGTAGTAATGAGGCG |
NGG |
2 |
0.5185 |
Tier II |
| 4 |
NC_000006.12 |
6 |
44819324 |
+ |
SUPT3H |
NNNGGGTTAGTGATGAGGCG |
NGG |
2 |
0.3175 |
Tier II |
| 5 |
NC_000001.11 |
1 |
201066094 |
- |
CACNA1S |
NNNGGGGTAAGGATGAGGCT |
NGG |
2 |
0.28 |
Tier II |
| 6 |
NC_000016.10 |
16 |
2168586 |
+ |
TRAF7 |
NNNGGGGTCCTGATGAGGCG |
NGG |
2 |
0.1905 |
Tier II |
| 7 |
NC_000011.10 |
11 |
4954247 |
+ |
MMP26 |
NNNGGGGAAATGATGGGGCG |
NGG |
2 |
0.1538 |
Tier II |
| 8 |
NC_000017.11 |
17 |
63697294 |
+ |
LIMD2 |
NNNGGGGTAATGATGAGGGG |
NGA |
2 |
0.0087 |
Tier II |
| 9 |
NC_000010.11 |
10 |
89748370 |
- |
KIF20B |
NNNGGGGTAATGAGGAGGAG |
NGG |
2 |
0.0 |
Tier II |
| 10 |
NC_000001.11 |
1 |
57898971 |
+ |
DAB1 |
NNNGGGGTAATGATGTGGTG |
NGG |
2 |
0.0 |
Tier II |
| 11 |
NC_000006.12 |
6 |
44819324 |
+ |
LOC101929770 |
NNNGGGTTAGTGATGAGGCG |
NGG |
2 |
0.3175 |
Tier III |
| 12 |
NC_000008.11 |
8 |
11378967 |
- |
FAM167A-AS1 |
NNNTTGGTAATGATGAGGCG |
NGG |
2 |
0.1091 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000073.6 |
7 |
27227519 |
+ |
Itpkc |
NNNGGGGTGATGATAAGGCG |
NGG |
2 |
0.6275 |
Tier I |
| 2 |
NC_000084.6 |
18 |
38923433 |
- |
Fgf1 |
NNNTGGGTAATGATGAGGCA |
NGG |
2 |
0.3409 |
Tier II |
| 3 |
NC_000084.6 |
18 |
38923433 |
- |
Arhgap26 |
NNNTGGGTAATGATGAGGCA |
NGG |
2 |
0.3409 |
Tier II |
| 4 |
NC_000084.6 |
18 |
38923433 |
- |
Gm5820 |
NNNTGGGTAATGATGAGGCA |
NGG |
2 |
0.3409 |
Tier II |
| 5 |
NC_000084.6 |
18 |
59047712 |
+ |
Adamts19 |
NNNGGGGTAAGGATGAGGAG |
NGG |
2 |
0.1714 |
Tier II |
| 6 |
NC_000082.6 |
16 |
94856925 |
- |
Kcnj6 |
NNNGGGGCAATGAGGAGGCG |
NGG |
2 |
0.0 |
Tier II |
| 7 |
NC_000083.6 |
17 |
32199438 |
+ |
Brd4 |
NNNGGGGTAAGGATGTGGCG |
NGG |
2 |
0.0 |
Tier II |
| 8 |
NC_000086.7 |
X |
3537476 |
+ |
Gm53053 |
NNNGGGGTCATGATGAGGCT |
NGG |
2 |
0.4 |
Tier III |
| 9 |
NC_000086.7 |
X |
3675992 |
+ |
Gm53053 |
NNNGGGGTCATGATGAGGCT |
NGG |
2 |
0.4 |
Tier III |
Other clones with same target sequence:
(none)
