Construct: sgRNA BRDN0001148041
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGTCTTGGTGCGATAACTGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PAK3 (5063)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75678
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000023.11 |
X |
111163014 |
- |
PAK3 |
NNNCTTGGTGCGATAACTGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000009.12 |
9 |
126421025 |
- |
MVB12B |
NNNCTTGGTGGGACAACTGG |
NGG |
2 |
0.0714 |
Tier II |
| 3 |
NC_000014.9 |
14 |
39706704 |
+ |
LOC105370461 |
NNNCTTGGTGCTATAACTGA |
NGG |
2 |
0.3606 |
Tier III |
| 4 |
NC_000002.12 |
2 |
194935497 |
- |
LOC105376755 |
NNNCTTGGTGTGATAACTGG |
NGG |
1 |
0.3077 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000086.7 |
X |
7842479 |
+ |
Otud5 |
NNNCTTGGTGCGACAACTGC |
NGG |
2 |
0.1224 |
Tier I |
| 2 |
NC_000069.6 |
3 |
85741644 |
- |
Fam160a1 |
NNNCTTGGTGCAATAACTTG |
NGG |
2 |
0.6222 |
Tier II |
Other clones with same target sequence:
(none)
