Construct: sgRNA BRDN0001148049
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ATGTGATCATCGATCTCTCA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PRKCE (5581)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76791
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
45843045 |
+ |
PRKCE |
NNNTGATCATCGATCTCTCA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000010.11 |
10 |
37156999 |
+ |
ANKRD30A |
NNNTGATCAATGATCTCTCA |
NGG |
2 |
0.2637 |
Tier II |
| 3 |
NC_000005.10 |
5 |
33877085 |
- |
ADAMTS12 |
NNNTGGTCATGGATCTCTCA |
NGG |
2 |
0.1786 |
Tier II |
| 4 |
NC_000009.12 |
9 |
84813461 |
+ |
NTRK2 |
NNNTGATCATTGTTCTCTCA |
NGG |
2 |
0.0923 |
Tier II |
| 5 |
NC_000015.10 |
15 |
95324831 |
+ |
LETR1 |
NNNAGATCATCTATCTCTCA |
NGG |
2 |
0.1832 |
Tier III |
| 6 |
NC_000011.10 |
11 |
61040979 |
- |
LOC105369325 |
NNNTGATCATCGATCTGCCA |
NGG |
2 |
0.0252 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000083.6 |
17 |
86353600 |
+ |
Prkce |
NNNTGATCATCGATCTCTCG |
NGA |
2 |
0.0531 |
Tier I |
| 2 |
NC_000077.6 |
11 |
81267373 |
+ |
Asic2 |
NNNTGATCATCAATCTCTCA |
NGG |
1 |
0.9333 |
Tier II |
| 3 |
NC_000067.6 |
1 |
91354604 |
+ |
Klhl30 |
NNNTCATCATCCATCTCTCA |
NGG |
2 |
0.416 |
Tier II |
| 4 |
NC_000082.6 |
16 |
34312135 |
+ |
Kalrn |
NNNTGATCATCGGACTCTCA |
NGG |
2 |
0.4037 |
Tier II |
| 5 |
NC_000077.6 |
11 |
121526168 |
+ |
Tbcd |
NNNTGAGCATCTATCTCTCA |
NGG |
2 |
0.2262 |
Tier II |
| 6 |
NC_000074.6 |
8 |
27201580 |
+ |
Got1l1 |
NNNTGATCATCCACCTCTCA |
NGG |
2 |
0.1513 |
Tier II |
| 7 |
NC_000086.7 |
X |
87125303 |
+ |
Il1rapl1 |
NNNTGATCATCGATCACTGA |
NGG |
2 |
0.1136 |
Tier II |
| 8 |
NC_000069.6 |
3 |
122007478 |
+ |
Arhgap29 |
NNNTTATCATCGATCTGTCA |
NGG |
2 |
0.0176 |
Tier II |
| 9 |
NC_000076.6 |
10 |
56446919 |
- |
Gm36595 |
NNNTGATCATCGATCTCTCT |
NTG |
2 |
0.0234 |
Tier III |
Other clones with same target sequence:
(none)
