Construct: sgRNA BRDN0001148050
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGAATATGTGGGCCTCGCTA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CDK13 (8621)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76626
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000007.14 |
7 |
39999376 |
+ |
CDK13 |
NNNATATGTGGGCCTCGCTA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000020.11 |
20 |
62924701 |
- |
DIDO1 |
NNNATATGTGAGCCTCGCAA |
NGG |
2 |
0.2857 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000079.6 |
13 |
17765079 |
- |
Cdk13 |
NNNATATGTGGGCCTCGCTA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000070.6 |
4 |
117552684 |
- |
Eri3 |
NNNATATGTGAGCCTCGTTA |
NGG |
2 |
0.6429 |
Tier II |
| 3 |
NC_000080.6 |
14 |
69536051 |
- |
LOC115488278 |
NNNATATGTGTGCATCGCTA |
NGG |
2 |
0.1346 |
Tier II |
Other clones with same target sequence:
(none)
