Construct: sgRNA BRDN0001148051

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
ACAAGCTCAAATTCTTCTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SNX16 (64089)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75837

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000008.11 8 81839868 - SNX16 NNNAGCTCAAATTCTTCTAA NGG 0 1.0 Tier I
2 NC_000012.12 12 119712679 + CIT NNNAGCTCAAATTCTTCCAC NGG 2 0.0974 Tier I
3 NC_000012.12 12 112021494 - ERP29 NNNAGCTCATATTCTTCTAA NGG 1 0.8824 Tier II
4 NC_000014.9 14 33670218 + NPAS3 NNNAGCTAAAATTCTTCTAA NGG 1 0.65 Tier II
5 NC_000010.11 10 129492299 - MGMT NNNAGCTCAAAACCTTCTAA NGG 2 0.6316 Tier II
6 NC_000007.14 7 48506646 + ABCA13 NNNAGCAGAAATTCTTCTAA NGG 2 0.5625 Tier II
7 NC_000015.10 15 28271331 + HERC2 NNNAGTTCTAATTCTTCTAA NGG 2 0.5571 Tier II
8 NC_000015.10 15 28628823 - LOC124903450 NNNAGTTCTAATTCTTCTAA NGG 2 0.5571 Tier II
9 NC_000005.10 5 120588504 - PRR16 NNNAGCTGAAATCCTTCTAA NGG 2 0.5075 Tier II
10 NC_000002.12 2 177903011 + PDE11A NNNAGCTCAAAATCATCTAA NGG 2 0.4632 Tier II
11 NC_000006.12 6 136686230 - MAP3K5 NNNAGCTCAAAGACTTCTAA NGG 2 0.3462 Tier II
12 NC_000007.14 7 83088414 + PCLO NNNTGCTCAAATTCTTCTTA NGG 2 0.3427 Tier II
13 NC_000002.12 2 109469003 + RANBP2 NNNAGCTCAAGGTCTTCTAA NGG 2 0.325 Tier II
14 NC_000002.12 2 109469003 + SH3RF3 NNNAGCTCAAGGTCTTCTAA NGG 2 0.325 Tier II
15 NC_000004.12 4 113605292 + CAMK2D NNNAGCCCAAATTCTTTTAA NGG 2 0.3208 Tier II
16 NC_000001.11 1 229523148 + ABCB10 NNNAGCTCAAATTCTACTAA NAG 2 0.2357 Tier II
17 NC_000003.12 3 3152886 + TRNT1 NNNAACTCAAATGCTTCTAA NGG 2 0.2261 Tier II
18 NC_000003.12 3 3152886 + CRBN NNNAACTCAAATGCTTCTAA NGG 2 0.2261 Tier II
19 NC_000006.12 6 20596174 - CDKAL1 NNNTGCTCAAATTCTGCTAA NGG 2 0.2203 Tier II
20 NC_000012.12 12 103293519 + C12orf42 NNNAGTTCAAATTCTTCTAC NGG 2 0.211 Tier II
21 NC_000003.12 3 140130612 + CLSTN2 NNNGGCTCACATTCTTCTAA NGG 2 0.2083 Tier II
22 NC_000012.12 12 44163072 - TMEM117 NNNAGCTCAATTTCTTCAAA NGG 2 0.2051 Tier II
23 NC_000021.9 21 33850133 - ITSN1 NNNAGATCAAATTCTTCTCA NGG 2 0.1921 Tier II
24 NC_000007.14 7 105246937 + SRPK2 NNNAGCTCAAATTCTTCCAA NAG 2 0.1111 Tier II
25 NC_000004.12 4 24147645 + PPARGC1A NNNAGCTCAAATTCTTCTGA NAG 2 0.0972 Tier II
26 NC_000003.12 3 151500157 + IGSF10 NNNAGCTCAAATTATTCTAC NGG 2 0.0795 Tier II
27 NC_000003.12 3 13003918 + IQSEC1 NNNATCTCAAATGCTTCTAA NGG 2 0.0783 Tier II
28 NC_000013.11 13 40571160 + FOXO1 NNNAGCTAAAATTCTTCTAA NTG 2 0.0253 Tier II
29 NC_000006.12 6 51981263 + PKHD1 NNNAGCTCAAATTCTTCAAA NGC 2 0.0148 Tier II
30 NC_000002.12 2 124332918 - CNTNAP5 NNNAGCTCAAATGCTTCTAA NGT 2 0.0042 Tier II
31 NC_000012.12 12 112021494 - LOC124903021 NNNAGCTCATATTCTTCTAA NGG 1 0.8824 Tier III
32 NC_000015.10 15 20466825 + HERC2P3 NNNAGTTCTAATTCTTCTAA NGG 2 0.5571 Tier III
33 NC_000015.10 15 22533614 - HERC2P2 NNNAGTTCTAATTCTTCTAA NGG 2 0.5571 Tier III
34 NC_000016.10 16 32180018 + HERC2P4 NNNAGTTCTAATTCTTCTAA NGG 2 0.5571 Tier III
35 NC_000016.10 16 32749410 - HERC2P5 NNNAGTTCTAATTCTTCTAA NGG 2 0.5571 Tier III
36 NC_000016.10 16 33120999 + HERC2P8 NNNAGTTCTAATTCTTCTAA NGG 2 0.5571 Tier III
37 NT_187383.1 16 1009730 - LOC102723753 NNNAGTTCTAATTCTTCTAA NGG 2 0.5571 Tier III
38 NC_000023.11 X 80333895 + CHMP1B2P NNNACCTCAAATACTTCTAA NGG 2 0.544 Tier III
39 NC_000012.12 12 58985914 + LRIG3-DT NNNAGCTCAAATTCTTCTTA NGG 1 0.5385 Tier III
40 NC_000002.12 2 13154960 - LOC105373436 NNNTGCTCAAAATCTTCTAA NGG 2 0.5091 Tier III
41 NC_000002.12 2 13154960 - LOC105373484 NNNTGCTCAAAATCTTCTAA NGG 2 0.5091 Tier III
42 NC_000009.12 9 103920278 - LOC105376192 NNNAGCTCAGATTCTTCTAG NGG 2 0.4248 Tier III
43 NC_000005.10 5 98106285 + LINC01846 NNNAGCCCAAATTCTTATAA NGG 2 0.3208 Tier III
44 NC_000006.12 6 142983984 - LINC01277 NNNAGTTCAAATTCTTCTAA NAG 2 0.2407 Tier III
45 NC_000006.12 6 137707074 + PTPN11P3 NNNAGCTCTATTTCTTCTAA NGG 2 0.1846 Tier III
46 NC_000005.10 5 23305007 + PTPN11P4 NNNAGCTCCATTTCTTCTAA NGG 2 0.1758 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000073.6 7 110044730 + Ipo7 NNNAGCTGAAATACTTCTAA NGG 2 0.4451 Tier I
2 NC_000071.6 5 115986576 - Cit NNNAGCTCAAATTCTTCCAC NGG 2 0.0974 Tier I
3 NC_000076.6 10 33087629 + Trdn NNNAGCTCAAATCTTTCTAA NGG 2 0.5789 Tier II
4 NC_000067.6 1 174764051 + Fmn2 NNNAGCTCAAGTTCATCTAA NGG 2 0.3763 Tier II
5 NC_000084.6 18 77661635 + 8030462N17Rik NNNAACTCAAATTATTCTAA NGG 2 0.3033 Tier II
6 NC_000068.7 2 30419706 - Ptpa NNNAACTCACATTCTTCTAA NGG 2 0.2889 Tier II
7 NC_000074.6 8 60669590 - Mfap3l NNNAGCTCAACTACTTCTAA NGG 2 0.2769 Tier II
8 NC_000069.6 3 62570004 - Gpr149 NNNGGCTCAAATTCTTCCAA NGG 2 0.2679 Tier II
9 NC_000083.6 17 30552999 + Btbd9 NNNAGCTCAAATTCTTCAGA NGG 2 0.25 Tier II
10 NC_000069.6 3 129326358 + Enpep NNNAGCTCAAACTCTTCTAA NAG 2 0.2456 Tier II
11 NC_000080.6 14 66116974 + Ephx2 NNNAGCTCACATTCTTCAAA NGG 2 0.2222 Tier II
12 NC_000072.6 6 121678771 + A2m NNNAGCTCACATTCTTTTAA NGG 2 0.1556 Tier II
13 NC_000081.6 15 33129340 - Cpq NNNAGCTAAAATTCTTCTCA NGG 2 0.1345 Tier II
14 NC_000069.6 3 59744941 + Aadacl2fm2 NNNAGTTCAAATTCTTCTAA NTG 2 0.0362 Tier II
15 NC_000079.6 13 48485333 - Zfp169 NNNAGCTCAAATGCTTCTAA NCG 2 0.028 Tier II
16 NC_000080.6 14 109402797 + Gm30216 NNNAGCAAAAATTCTTCTAA NGG 2 0.5688 Tier III
17 NC_000076.6 10 15564218 + Gm46188 NNNAGTTCAAATTATTCTAA NGG 2 0.325 Tier III
18 NC_000084.6 18 43451979 - Gm41714 NNNAGCTCAAATAATTCTAA NGG 2 0.2423 Tier III
19 NC_000078.6 12 5660592 - Gm32398 NNNAGCTCAAATGCTACTAA NGG 2 0.2372 Tier III
20 NC_000077.6 11 18486973 - Gm12022 NNNAGCTGAAATTCTTCTAA NGT 2 0.0104 Tier III
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Other clones with same target sequence:

(none)