Construct: sgRNA BRDN0001148055
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GCTGGCAGAACCCATCCTCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ROR2 (4920)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77939
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000009.12 |
9 |
91737504 |
+ |
ROR2 |
NNNGGCAGAACCCATCCTCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000010.11 |
10 |
79152990 |
- |
ZMIZ1 |
NNNGACAGAACCCATCCTCT |
NGG |
2 |
0.6067 |
Tier II |
| 3 |
NC_000016.10 |
16 |
73495549 |
- |
ZFHX3 |
NNNGGCAGAAGCCATACTCG |
NGG |
2 |
0.25 |
Tier II |
| 4 |
NC_000015.10 |
15 |
101319060 |
- |
PCSK6 |
NNNGGCAGAAGCCATCCACG |
NGG |
2 |
0.1667 |
Tier II |
| 5 |
NC_000006.12 |
6 |
168307294 |
+ |
DACT2 |
NNNGGCAGAACCCATCTGCG |
NGG |
2 |
0.1556 |
Tier II |
| 6 |
NC_000012.12 |
12 |
99099017 |
- |
ANKS1B |
NNNGGCAGAACCCATCCTGG |
NGA |
2 |
0.0087 |
Tier II |
| 7 |
NC_000012.12 |
12 |
42535037 |
+ |
PRICKLE1 |
NNNGGCAGAACCCACCCTCG |
NGT |
2 |
0.0044 |
Tier II |
| 8 |
NC_000008.11 |
8 |
93134360 |
+ |
LINC02906 |
NNNGGCAGAGCCCATCCTCG |
NGC |
2 |
0.0123 |
Tier III |
| 9 |
NC_000012.12 |
12 |
99099017 |
- |
LOC101928937 |
NNNGGCAGAACCCATCCTGG |
NGA |
2 |
0.0087 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000080.6 |
14 |
72450400 |
- |
Gm9195 |
NNNGGCAGCACCCATCCTCT |
NGG |
2 |
0.4 |
Tier I |
| 2 |
NC_000077.6 |
11 |
4143876 |
- |
Ccdc157 |
NNNGGCAGGACCCATCCTGG |
NGG |
2 |
0.0833 |
Tier I |
| 3 |
NC_000078.6 |
12 |
45029319 |
+ |
Stxbp6 |
NNNGGCAGATCCCATCCTCA |
NGG |
2 |
0.8272 |
Tier II |
| 4 |
NC_000076.6 |
10 |
106839853 |
- |
Ppfia2 |
NNNGACAGGACCCATCCTCG |
NGG |
2 |
0.5778 |
Tier II |
| 5 |
NC_000072.6 |
6 |
116450129 |
+ |
Alox5 |
NNNGGCAGAACCCATCCCCA |
NGG |
2 |
0.4018 |
Tier II |
| 6 |
NC_000074.6 |
8 |
79057352 |
+ |
Zfp827 |
NNNGGCAGAACCCATCCTGG |
NGG |
1 |
0.125 |
Tier II |
| 7 |
NC_000071.6 |
5 |
107239162 |
+ |
Tgfbr3 |
NNNGGCAGAACCCATCCTCC |
NAG |
2 |
0.1111 |
Tier II |
| 8 |
NC_000076.6 |
10 |
56101657 |
- |
Msl3l2 |
NNNGGCACAACCCATCCTGG |
NGG |
2 |
0.0769 |
Tier II |
| 9 |
NC_000076.6 |
10 |
56101657 |
- |
Tbc1d32 |
NNNGGCACAACCCATCCTGG |
NGG |
2 |
0.0769 |
Tier II |
| 10 |
NC_000070.6 |
4 |
125587933 |
+ |
Grik3 |
NNNGGCAGAACCGATCCCCG |
NGG |
2 |
0.0584 |
Tier II |
| 11 |
NC_000072.6 |
6 |
113981831 |
- |
Atp2b2 |
NNNGGCAGAACCCAGCTTCG |
NGG |
2 |
0.0233 |
Tier II |
| 12 |
NC_000074.6 |
8 |
125501445 |
+ |
Sipa1l2 |
NNNGTCAGAACCCATCCTCG |
NGT |
2 |
0.0048 |
Tier II |
| 13 |
NC_000067.6 |
1 |
186414168 |
- |
Gm35341 |
NNNGGCAGAAGCCATCCTCT |
NGG |
2 |
0.175 |
Tier III |
| 14 |
NC_000076.6 |
10 |
56101657 |
- |
Gm29794 |
NNNGGCACAACCCATCCTGG |
NGG |
2 |
0.0769 |
Tier III |
Other clones with same target sequence:
(none)
