Construct: sgRNA BRDN0001148059

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GCAACTGCACACATTTACCC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ALPK2 (115701)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77521

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000018.10 18 58579925 - ALPK2 NNNACTGCACACATTTACCC NGG 0 1.0 Tier I
2 NC_000011.10 11 102872968 - MMP12 NNNACTGGACACATCTACCC NGG 2 0.1753 Tier I
3 NC_000011.10 11 32830573 + PRRG4 NNNACTGCCCACAGTTACCC NGG 2 0.0 Tier I
4 NC_000016.10 16 84326990 - WFDC1 NNNGCTGCCCACATTTACCC NGG 2 0.3571 Tier II
5 NC_000002.12 2 3202998 - EIPR1 NNNATTGCACACATTTACCT NGG 2 0.1909 Tier II
6 NC_000008.11 8 118007568 + EXT1 NNNACGGCACACATTTACCT NGG 2 0.1714 Tier II
7 NC_000001.11 1 165216317 + LMX1A NNNACTGCACAGATTTGCCC NGG 2 0.0784 Tier II
8 NC_000022.11 22 33768410 + LARGE1 NNNACTGCACACCTTTACCC NAG 2 0.0546 Tier II
9 NC_000002.12 2 133217842 + NCKAP5 NNNACTGCACACATATACCC NGA 2 0.0402 Tier II
10 NC_000008.11 8 80783386 - ZNF704 NNNACAGCACACATTTACCC NTG 2 0.0338 Tier II
11 NC_000023.11 X 92256614 - PCDH11X NNNACGGCACACATTTACCC NTG 2 0.0223 Tier II
12 NC_000003.12 3 125036561 - HEG1 NNNACTGCAGACATTTACCC NTG 2 0.0152 Tier II
13 NC_000006.12 6 138090473 + PERP NNNACTGCACACATGTCCCC NGG 2 0.0088 Tier II
14 NC_000012.12 12 117825366 - KSR2 NNNCCTGCACACATTTACCC NGC 2 0.0078 Tier II
15 NC_000011.10 11 32830573 + CCDC73 NNNACTGCCCACAGTTACCC NGG 2 0.0 Tier II
16 NC_000008.11 8 102536863 + LOC124901998 NNNACTGCACAAATTAACCC NGG 2 0.6494 Tier III
17 NC_000015.10 15 38886374 - LOC105370777 NNNACGGCACACATTTACCT NGG 2 0.1714 Tier III
18 NC_000005.10 5 176181389 + CEP192P1 NNNACTGCTCACACTTACCC NGG 2 0.1714 Tier III
19 NC_000001.11 1 165216317 + LMX1A-AS1 NNNACTGCACAGATTTGCCC NGG 2 0.0784 Tier III
20 NC_000018.10 18 38709448 + LOC105372076 NNNCCTGCACACATTTCCCC NGG 2 0.0623 Tier III
21 NC_000018.10 18 1100044 - LOC105371953 NNNACTGCACTCATTTTCCC NGG 2 0.041 Tier III
22 NC_000004.12 4 142925303 + USP38-DT NNNAGTGCACACATTTACCC NGT 2 0.0097 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000067.6 1 70461068 - Spag16 NNNACTGCTCACATTTACCC NAG 2 0.1556 Tier I
2 NC_000077.6 11 70659578 - Eno3 NNNACAGCACACATTCACCC NGG 2 0.5778 Tier II
3 NC_000083.6 17 24858137 - Hagh NNNGCTGCACACATTAACCC NGG 2 0.5682 Tier II
4 NC_000068.7 2 132014447 - Rassf2 NNNACAGCACACATTTACTC NGG 2 0.4 Tier II
5 NC_000077.6 11 101521459 + Brca1 NNNCCTCCACACATTTACCC NGG 2 0.2426 Tier II
6 NC_000070.6 4 47472569 - Alg2 NNNAATGCAGACATTTACCC NGG 2 0.2222 Tier II
7 NC_000082.6 16 16486175 + Fgd4 NNNACTGTACACATTTCCCC NGG 2 0.1544 Tier II
8 NC_000072.6 6 99360358 + Foxp1 NNNACTGCACACATCTACTC NGG 2 0.1259 Tier II
9 NC_000070.6 4 85219408 - Sh3gl2 NNNACTGCACACATTTACTC NAG 2 0.1197 Tier II
10 NC_000071.6 5 127409374 + Tmem132c NNNACTGCACACGTTTACGC NGG 2 0.0815 Tier II
11 NC_000069.6 3 108688680 + Gpsm2 NNNACTGCACCCATTTCCCC NGG 2 0.0706 Tier II
12 NC_000070.6 4 36631508 + Lingo2 NNNACTGCACTCATTTGCCC NGG 2 0.0543 Tier II
13 NC_000080.6 14 79644853 + Cnmd NNNACTGCACTCATTTTCCC NGG 2 0.041 Tier II
14 NC_000077.6 11 86115319 - Brip1 NNNACTGCACACATGTACCA NGG 2 0.025 Tier II
15 NC_000067.6 1 135166010 - Gpr37l1 NNNACTGCACACATCTACCC NTG 2 0.0106 Tier II
16 NC_000073.6 7 19041662 + Sympk NNNACTGCACACATGTGCCC NGG 2 0.0088 Tier II
17 NC_000082.6 16 11902626 - Cpped1 NNNACTGCACACATTTACCC NTC 2 0.0 Tier II
18 NC_000069.6 3 130660218 + Gm36373 NNNACAGCACACATTCACCC NGG 2 0.5778 Tier III
19 NC_000068.7 2 120853165 - AV039307 NNNACTGCACACATTCACTC NGG 2 0.3077 Tier III
20 NC_000082.6 16 11902626 - Gm9961 NNNACTGCACACATTTACCC NTC 2 0.0 Tier III
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Other clones with same target sequence:

(none)