Construct: sgRNA BRDN0001148060

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AGGATCCTGGGACATTAGGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EPHB1 (2047)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77534

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000003.12 3 134951547 - EPHB1 NNNATCCTGGGACATTAGGG NGG 0 1.0 Tier I
2 NC_000001.11 1 146156467 + NOTCH2NLA NNNATCCTGGGACACTAGGG NGC 2 0.0061 Tier I
3 NC_000001.11 1 148607397 + NOTCH2NLB NNNATCCTGGGACACTAGGG NGC 2 0.0061 Tier I
4 NC_000002.12 2 229040964 + PID1 NNNACCCTGGGACATTAGAG NGG 2 0.7143 Tier II
5 NC_000020.11 20 38026726 + TTI1 NNNATCCTGGAACATTAAGG NGG 2 0.6923 Tier II
6 NC_000011.10 11 83600056 - DLG2 NNNAACCTGGGACATTAGGG NGG 1 0.5 Tier II
7 NC_000005.10 5 6732862 - TENT4A NNNATCCTGGGACATTAGCA NGG 2 0.4203 Tier II
8 NC_000016.10 16 15446668 - BMERB1 NNNATCCTGGGAAATTAGGA NGG 2 0.3606 Tier II
9 NC_000016.10 16 15446668 - MPV17L-BMERB1 NNNATCCTGGGAAATTAGGA NGG 2 0.3606 Tier II
10 NC_000006.12 6 128883757 + LAMA2 NNNATCCTGGGACTTTATGG NGG 2 0.3556 Tier II
11 NC_000015.10 15 75467085 - PTPN9 NNNAGCCTGGGACATGAGGG NGG 2 0.2215 Tier II
12 NC_000003.12 3 195893031 + TNK2 NNNCTCCTGGGACTTTAGGG NGG 2 0.1882 Tier II
13 NC_000006.12 6 155219697 + TIAM2 NNNATCCTTGGTCATTAGGG NGG 2 0.1778 Tier II
14 NC_000005.10 5 127421490 - MEGF10 NNNATCCTGGGAAATGAGGG NGG 2 0.1331 Tier II
15 NC_000018.10 18 57438916 - ONECUT2 NNNAGCCTGGGACATTCGGG NGG 2 0.1129 Tier II
16 NC_000002.12 2 182996975 + NCKAP1 NNNATCCTGGGAAATTTGGG NGG 2 0.0513 Tier II
17 NC_000001.11 1 212585959 - ATF3 NNNATCCTGGGTCATTTGGG NGG 2 0.0444 Tier II
18 NC_000008.11 8 70804140 + XKR9 NNNATCCTGGGACATTAGGA NGC 2 0.0208 Tier II
19 NC_000015.10 15 42386949 - CAPN3 NNNATCCTGGGACATGAGGG NGC 2 0.0077 Tier II
20 NC_000001.11 1 119996984 + NOTCH2 NNNATCCTGGGACACTAGGG NGC 2 0.0061 Tier II
21 NC_000001.11 1 120793510 - NBPF26 NNNATCCTGGGACACTAGGG NGC 2 0.0061 Tier II
22 NC_000001.11 1 120793510 - NOTCH2NLR NNNATCCTGGGACACTAGGG NGC 2 0.0061 Tier II
23 NC_000001.11 1 149463840 - NOTCH2NLC NNNATCCTGGGACACTAGGG NGC 2 0.0061 Tier II
24 NC_000022.11 22 43276568 - SCUBE1 NNNATCCTGGGTCATTAGGG NGT 2 0.0054 Tier II
25 NC_000008.11 8 126655545 - LOC105375751 NNNATCCTGGGACATTTGGC NGG 2 0.0571 Tier III
26 NC_000008.11 8 78876464 - MITA1 NNNATCCTGGGACAGAAGGG NGG 2 0.0455 Tier III
27 NC_000001.11 1 151942061 + LOC124904421 NNNATCCTAGGACATTAGGG NTG 2 0.025 Tier III
28 NC_000022.11 22 43276568 - SCUBE1-AS1 NNNATCCTGGGTCATTAGGG NGT 2 0.0054 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000073.6 7 49464071 + Nav2 NNNACTCTGGGACATTAGGG NGG 2 0.9286 Tier II
2 NC_000080.6 14 70545775 - Reep4 NNNATCCTGGGACAAAAGGG NGG 2 0.5263 Tier II
3 NC_000077.6 11 89597558 + Ankfn1 NNNATCCTGGGACTTCAGGG NGG 2 0.3556 Tier II
4 NC_000067.6 1 156816370 - Ralgps2 NNNATACTGGGACCTTAGGG NGG 2 0.199 Tier II
5 NC_000072.6 6 145938187 - Sspn NNNATCCTGGGACGTGAGGG NGG 2 0.1615 Tier II
6 NC_000082.6 16 91373591 - Ifnar2 NNNATCCTGGGACCTTAGGT NGG 2 0.15 Tier II
7 NC_000082.6 16 90788851 - Urb1 NNNATCCTGGGACATTGGGT NGG 2 0.1235 Tier II
8 NC_000067.6 1 153901664 + Glul NNNATCCGGGGAGATTAGGG NGG 2 0.1 Tier II
9 NC_000078.6 12 101659713 + Tc2n NNNATCCTGGGACCTTTGGG NGG 2 0.0286 Tier II
10 NC_000068.7 2 125949819 + Galk2 NNNATCCTGGGACATTAAGG NTG 2 0.027 Tier II
11 NC_000071.6 5 130040764 - Crcp NNNATCCTGGGACATGAGGG NGA 2 0.024 Tier II
12 NC_000076.6 10 80060121 - Sbno2 NNNATCCTGGGACAGTTGGG NGG 2 0.0067 Tier II
13 NC_000075.6 9 100179518 - 4930540E01Rik NNNATCCTAGGACATTAGTG NGG 2 0.4286 Tier III
14 NC_000075.6 9 94114821 - Gm31847 NNNATCCTGGGACACTAGGA NGG 2 0.2557 Tier III
15 NC_000075.6 9 94114821 - Gm39403 NNNATCCTGGGACACTAGGA NGG 2 0.2557 Tier III
16 NC_000082.6 16 91373591 - Gm36587 NNNATCCTGGGACCTTAGGT NGG 2 0.15 Tier III
17 NC_000077.6 11 107195483 + Gm39425 NNNATCCTGGGAGTTTAGGG NGG 2 0.0727 Tier III
18 NC_000086.7 X 99560231 - Gm31155 NNNATCCTGGGAGATGAGGG NGG 2 0.0472 Tier III
Download CSV

Other clones with same target sequence:

(none)