Construct: sgRNA BRDN0001148062

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TCCCTGTGCAATTACCTCAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TBCK (93627)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75565

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000004.12 4 106251985 + TBCK NNNCTGTGCAATTACCTCAG NGG 0 1.0 Tier I
2 NC_000002.12 2 197495308 + HSPD1 NNNCTGTGCAATTTCTTCAG NGG 2 0.1641 Tier I
3 NC_000008.11 8 59070850 + TOX NNNATGTACAATTACCTCAG NGG 2 0.8421 Tier II
4 NC_000002.12 2 149384612 - LYPD6 NNNCTGTGCTATTACCTCTG NGG 2 0.4751 Tier II
5 NC_000001.11 1 31208657 - NKAIN1 NNNCTGTGCACTTACATCAG NGG 2 0.4 Tier II
6 NC_000020.11 20 61433279 + CDH4 NNNCTGTGAAATTACCTCGG NGG 2 0.3214 Tier II
7 NC_000020.11 20 61862984 - CDH4 NNNCTGTGCATTAACCTCAG NGG 2 0.213 Tier II
8 NC_000005.10 5 84265864 + EDIL3 NNNCTGAGCAATTAACTCAG NGG 2 0.1944 Tier II
9 NC_000008.11 8 39673022 + ADAM18 NNNTTGTGCAATTCCCTCAG NGG 2 0.1714 Tier II
10 NC_000005.10 5 21883087 - CDH12 NNNCTGTGCAATTTCTTCAG NGG 2 0.1641 Tier II
11 NC_000004.12 4 175770742 + GPM6A NNNCTGTGCAATTTCCTCAG NAG 2 0.1383 Tier II
12 NC_000011.10 11 74913256 - XRRA1 NNNCTCTGCAATTACCTCAG NGA 2 0.0473 Tier II
13 NC_000019.10 19 31397620 + TSHZ3-AS1 NNNCTGTGGAAATACCTCAG NGG 2 0.4952 Tier III
14 NC_000020.11 20 61433279 + LOC105372703 NNNCTGTGAAATTACCTCGG NGG 2 0.3214 Tier III
15 NC_000007.14 7 27734335 + TAX1BP1-AS1 NNNCTGAGCAATTACTTCAG NGG 2 0.2692 Tier III
16 NC_000005.10 5 21883087 - HSPD1P1 NNNCTGTGCAATTTCTTCAG NGG 2 0.1641 Tier III
17 NC_000012.12 12 8015516 - HSPD1P12 NNNCTGTGCAATTTCTTCAG NGG 2 0.1641 Tier III
18 NC_000012.12 12 56511501 - HSPD1P4 NNNCTGTGCAATTTCTTCAG NGG 2 0.1641 Tier III
19 NC_000006.12 6 155795962 + LOC105378072 NNNCTGTGAAATTAGCTCAG NGG 2 0.0429 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000076.6 10 29346988 + Rnf146 NNNCTGTGCAACTACCACAG NGG 2 0.5053 Tier I
2 NC_000069.6 3 132715612 - Tbck NNNTTGTGCAATTACCTCAG NTG 2 0.0312 Tier I
3 NC_000073.6 7 43694986 + Klk14 NNNCTGTGCAATTACCACAG NTG 2 0.0208 Tier I
4 NC_000080.6 14 29680648 - Cacna2d3 NNNCTCTGAAATTACCTCAG NGG 2 0.5844 Tier II
5 NC_000068.7 2 58642840 - Upp2 NNNTTGTGGAATTACCTCAG NGG 2 0.4952 Tier II
6 NC_000070.6 4 102110096 + Pde4b NNNCTCTGCAGTTACCTCAG NGG 2 0.4432 Tier II
7 NC_000068.7 2 73368274 + Gpr155 NNNCAGTGCAATAACCTCAG NGG 2 0.3462 Tier II
8 NC_000070.6 4 121616652 + Gm12887 NNNGTGTGCAATAACCTCAG NGG 2 0.3462 Tier II
9 NC_000085.6 19 38305337 + Lgi1 NNNCTGTGCATTTACATCAG NGG 2 0.3077 Tier II
10 NC_000080.6 14 117505156 + Gpc6 NNNCTGTGCAATGACCTCAG NGG 1 0.2609 Tier II
11 NC_000077.6 11 68456591 - Pik3r5 NNNGTGTGCAAGTACCTCAG NGG 2 0.25 Tier II
12 NC_000071.6 5 106621381 - Zfp644 NNNCTGTGGATTTACCTCAG NGG 2 0.1905 Tier II
13 NC_000072.6 6 49155575 + Igf2bp3 NNNCTGGGCATTTACCTCAG NGG 2 0.181 Tier II
14 NC_000069.6 3 55275130 + Dclk1 NNNCTGTGCAAGGACCTCAG NGG 2 0.1304 Tier II
15 NC_000080.6 14 99220106 - Pibf1 NNNCTGTGCCATTCCCTCAG NGG 2 0.0714 Tier II
16 NC_000086.7 X 169798162 + Mid1 NNNCTGTGCAATTTCCGCAG NGG 2 0.0627 Tier II
17 NC_000081.6 15 52333859 - Slc30a8 NNNCTGTGCAATCAGCTCAG NGG 2 0.0395 Tier II
18 NC_000084.6 18 24839069 + Fhod3 NNNCTGTGCAATTACCTCAA NGC 2 0.0208 Tier II
19 NC_000069.6 3 100580836 + Man1a2 NNNCTGTGCAATGACCTCAG NGC 2 0.0058 Tier II
20 NC_000067.6 1 93170252 + Crocc2 NNNCTGTGCAATTACCTGAG NTG 2 0.0052 Tier II
21 NC_000078.6 12 46008981 - Gm33811 NNNCTGTGCTGTTACCTCAG NGG 2 0.5735 Tier III
22 NC_000071.6 5 93391369 - Kat2b-ps NNNCTGTGCAGTAACCTCAG NGG 2 0.45 Tier III
23 NC_000068.7 2 73368274 + Gm13709 NNNCAGTGCAATAACCTCAG NGG 2 0.3462 Tier III
24 NC_000078.6 12 72817976 - Gm33785 NNNCTGTACAATTAGCTCAG NGG 2 0.05 Tier III
25 NC_000067.6 1 51331917 + 9330175M20Rik NNNCTGTGCACTTACCTCAG NGT 2 0.0065 Tier III
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Other clones with same target sequence:

(none)