Construct: sgRNA BRDN0001148065
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGAATGGAATGCACCAGTGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NTRK2 (4915)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76468
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000009.12 |
9 |
84727700 |
- |
NTRK2 |
NNNATGGAATGCACCAGTGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000023.11 |
X |
65516043 |
- |
LAS1L |
NNNATGGAATGCACCAGTGA |
NGG |
1 |
0.9375 |
Tier II |
| 3 |
NC_000019.10 |
19 |
11397682 |
- |
RGL3 |
NNNATTGAATGCACCAGTGG |
NGG |
1 |
0.6667 |
Tier II |
| 4 |
NC_000019.10 |
19 |
34633127 |
- |
SCGB2B2 |
NNNATGGAAAGCACCAGTGT |
NGG |
2 |
0.6 |
Tier II |
| 5 |
NC_000005.10 |
5 |
142608167 |
- |
FGF1 |
NNNATTGAATGCACCAGTTG |
NGG |
2 |
0.4444 |
Tier II |
| 6 |
NC_000003.12 |
3 |
71511411 |
- |
FOXP1 |
NNNATGGAATGCCCCAATGG |
NGG |
2 |
0.1965 |
Tier II |
| 7 |
NC_000014.9 |
14 |
47539871 |
- |
MDGA2 |
NNNATGGAATGAACCAGTGG |
NAG |
2 |
0.1852 |
Tier II |
| 8 |
NC_000004.12 |
4 |
95213012 |
- |
UNC5C |
NNNATGGAATGCATCGGTGG |
NGG |
2 |
0.141 |
Tier II |
| 9 |
NC_000022.11 |
22 |
48578039 |
- |
TAFA5 |
NNNCTGGAATGCACCAGGGG |
NGG |
2 |
0.1176 |
Tier II |
| 10 |
NC_000010.11 |
10 |
48637925 |
- |
ARHGAP22 |
NNNATGGAATGCACAAGTGG |
NTG |
2 |
0.0087 |
Tier II |
| 11 |
NC_000003.12 |
3 |
65768693 |
+ |
MAGI1 |
NNNATGAAATGCAGCAGTGG |
NGG |
2 |
0.0 |
Tier II |
| 12 |
NC_000020.11 |
20 |
58367163 |
+ |
RAB22A |
NNNATGGAATGCTGCAGTGG |
NGG |
2 |
0.0 |
Tier II |
| 13 |
NC_000013.11 |
13 |
107252593 |
- |
NALF1 |
NNNATGGCATGCACCTGTGG |
NGG |
2 |
0.0 |
Tier II |
| 14 |
NC_000019.10 |
19 |
34633127 |
- |
SCGB1B2P |
NNNATGGAAAGCACCAGTGT |
NGG |
2 |
0.6 |
Tier III |
| 15 |
NC_000019.10 |
19 |
34633127 |
- |
ZNF807P |
NNNATGGAAAGCACCAGTGT |
NGG |
2 |
0.6 |
Tier III |
| 16 |
NC_000003.12 |
3 |
107274816 |
+ |
DUBR |
NNNAAGGAATGCACCAGTGA |
NGG |
2 |
0.4688 |
Tier III |
| 17 |
NC_000005.10 |
5 |
5793940 |
+ |
LOC105374636 |
NNNCTGAAATGCACCAGTGG |
NGG |
2 |
0.3529 |
Tier III |
| 18 |
NC_000003.12 |
3 |
72089908 |
+ |
LINC00877 |
NNNATGGAATGTACAAGTGG |
NGG |
2 |
0.1197 |
Tier III |
| 19 |
NC_000022.11 |
22 |
48578039 |
- |
LOC124900480 |
NNNCTGGAATGCACCAGGGG |
NGG |
2 |
0.1176 |
Tier III |
| 20 |
NC_000003.12 |
3 |
65768693 |
+ |
LOC107986018 |
NNNATGAAATGCAGCAGTGG |
NGG |
2 |
0.0 |
Tier III |
| 21 |
NC_000002.12 |
2 |
13599179 |
+ |
LOC105373438 |
NNNATTGAATGCAGCAGTGG |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000079.6 |
13 |
58861296 |
- |
Ntrk2 |
NNNATGGAATGCACCAGTGG |
NGA |
1 |
0.0694 |
Tier I |
| 2 |
NC_000069.6 |
3 |
38458191 |
+ |
Ankrd50 |
NNNAAAGAATGCACCAGTGG |
NGG |
2 |
0.5 |
Tier II |
| 3 |
NC_000073.6 |
7 |
138960401 |
+ |
Jakmip3 |
NNNAAGGAATGCATCAGTGG |
NGG |
2 |
0.3667 |
Tier II |
| 4 |
NC_000069.6 |
3 |
24709463 |
- |
Naaladl2 |
NNNATAGAATGCACCAGGGG |
NGG |
2 |
0.3333 |
Tier II |
| 5 |
NC_000068.7 |
2 |
169923088 |
- |
Tshz2 |
NNNGTGGAATGCACCAGTGG |
NAG |
2 |
0.162 |
Tier II |
| 6 |
NC_000073.6 |
7 |
44260743 |
- |
Acp4 |
NNNAGGGAATGCACCACTGG |
NGG |
2 |
0.1506 |
Tier II |
| 7 |
NC_000082.6 |
16 |
32484726 |
- |
Slc51a |
NNNATGGAATGAACCAGTGG |
NGT |
2 |
0.0115 |
Tier II |
| 8 |
NC_000078.6 |
12 |
34559004 |
- |
Hdac9 |
NNNATGGAATGCAGCAGTGA |
NGG |
2 |
0.0 |
Tier II |
| 9 |
NC_000084.6 |
18 |
52699260 |
- |
Gm41725 |
NNNATGGAATGAACCAGTGG |
NGG |
1 |
0.7143 |
Tier III |
Other clones with same target sequence:
(none)
