Construct: sgRNA BRDN0001148068

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
ACTTCAGACTCACGACGTAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CASK (8573)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77403

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000023.11 X 41578507 - CASK NNNTCAGACTCACGACGTAG NGG 0 1.0 Tier I
2 NC_000001.11 1 35718711 + C1orf216 NNNTCGGACTCACGGCGTAG NGG 2 0.4643 Tier II
3 NC_000010.11 10 124019354 + CHST15 NNNTCAGACTCACTCCGTAG NGG 2 0.0727 Tier II
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000086.7 X 13561682 - Cask NNNTCAGACTCATGATGTAG NGG 2 0.2154 Tier I
2 NC_000078.6 12 110882381 + Cinp NNNTCAGACTCACTACGTAA NGG 2 0.25 Tier II
3 NC_000073.6 7 49839993 - Prmt3 NNNTCAGACTCACTAGGTAG NGG 2 0.041 Tier II
4 NC_000075.6 9 61094611 + Gm34004 NNNTCAGACTCACGATGTAG NTG 2 0.012 Tier III
Download CSV

Other clones with same target sequence:

(none)