Construct: sgRNA BRDN0001148069

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CATGATCTCAGATTAGAGAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TEC (7006)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77024

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000004.12 4 48167847 - TEC NNNGATCTCAGATTAGAGAG NGG 0 1.0 Tier I
2 NC_000008.11 8 68172638 - PREX2 NNNGATCTCAGATTAAAGAT NGG 2 0.7 Tier II
3 NC_000009.12 9 2049912 + SMARCA2 NNNGATCAAAGATTAGAGAG NGG 2 0.6857 Tier II
4 NC_000019.10 19 50976461 - KLK7 NNNGATCTAAGATTGGAGAG NGG 2 0.5571 Tier II
5 NC_000010.11 10 128074022 - PTPRE NNNGATCACAGATTAGATAG NGG 2 0.5333 Tier II
6 NC_000013.11 13 25716392 - ATP8A2 NNNGATCTCAGATTAGACAA NGG 2 0.4464 Tier II
7 NC_000015.10 15 83712916 - ADAMTSL3 NNNGGTCTGAGATTAGAGAG NGG 2 0.4457 Tier II
8 NC_000021.9 21 25956939 - APP NNNGATCTCACATTAAAGAG NGG 2 0.4286 Tier II
9 NC_000002.12 2 30807371 + CAPN13 NNNGCTCTCAGGTTAGAGAG NGG 2 0.3611 Tier II
10 NC_000002.12 2 172699945 + PDK1 NNNTACCTCAGATTAGAGAG NGG 2 0.3306 Tier II
11 NC_000012.12 12 56770112 + HSD17B6 NNNGCTCTCAGATTGGAGAG NGG 2 0.325 Tier II
12 NC_000011.10 11 70774677 + SHANK2 NNNGCTCTGAGATTAGAGAG NGG 2 0.3095 Tier II
13 NC_000011.10 11 33411232 - KIAA1549L NNNCATCTCAGATTAGAGGG NGG 2 0.1985 Tier II
14 NC_000003.12 3 30612443 + TGFBR2 NNNGATATCAGATTTGAGAG NGG 2 0.15 Tier II
15 NC_000003.12 3 134985618 - EPHB1 NNNGATCTCATTTTAGAGAG NGG 2 0.1282 Tier II
16 NC_000009.12 9 72749015 - TMC1 NNNGTTCTCAGATTTGAGAG NGG 2 0.0727 Tier II
17 NC_000005.10 5 72153278 + MAP1B NNNGATCTCACATGAGAGAG NGG 2 0.0 Tier II
18 NC_000014.9 14 59349680 + DAAM1 NNNGATCTCAGATGAGAGAG NGA 2 0.0 Tier II
19 NC_000005.10 5 164488199 + LOC102546299 NNNGACCTCAGATAAGAGAG NGG 2 0.5628 Tier III
20 NC_000005.10 5 164488199 + LINC03000 NNNGACCTCAGATAAGAGAG NGG 2 0.5628 Tier III
21 NC_000011.10 11 109764615 + LINC02715 NNNGCTCTGAGATTAGAGAG NGG 2 0.3095 Tier III
22 NC_000016.10 16 80841901 + ARLNC1 NNNGATCTCACCTTAGAGAG NGG 2 0.1128 Tier III
23 NC_000021.9 21 18577777 - MIR548XHG NNNGATCTCAGTGTAGAGAG NGG 2 0.087 Tier III
24 NC_000004.12 4 18743200 - LOC105374510 NNNGATTTCAGATTAGAGAG NTG 2 0.0317 Tier III
25 NC_000014.9 14 29187863 + LOC107984685 NNNGTTCTCAGATTAGAGAG NGA 2 0.0253 Tier III
26 NC_000005.10 5 147187263 + STK32A-AS1 NNNGATCTCAGATTACAGAG NGA 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000071.6 5 72782072 - Tec NNNGACCTCAGGTTAGAGAG NGG 2 0.6566 Tier I
2 NC_000077.6 11 77274159 + Ssh2 NNNGACCTCAAATTAGAGAG NGG 2 0.9091 Tier II
3 NC_000075.6 9 84004563 + Bckdhb NNNGATCTCTAATTAGAGAG NGG 2 0.8824 Tier II
4 NC_000071.6 5 31700721 + Babam2 NNNGACCTTAGATTAGAGAG NGG 2 0.7955 Tier II
5 NC_000070.6 4 46003084 + Tdrd7 NNNGACCTCAGACTAGAGAG NGG 2 0.7177 Tier II
6 NC_000069.6 3 83225115 - Dchs2 NNNGATCTAAGGTTAGAGAG NGG 2 0.619 Tier II
7 NC_000085.6 19 24731697 - Pgm5 NNNGATCTCAGGCTAGAGAG NGG 2 0.5702 Tier II
8 NC_000080.6 14 70479786 + Bmp1 NNNGAGTTCAGATTAGAGAG NGG 2 0.4643 Tier II
9 NC_000082.6 16 40831396 + Lsamp NNNCATCACAGATTAGAGAG NGG 2 0.4235 Tier II
10 NC_000084.6 18 5296235 - Zfp438 NNNGAGCTGAGATTAGAGAG NGG 2 0.3537 Tier II
11 NC_000067.6 1 118394264 - Clasp1 NNNGATGTCAGGTTAGAGAG NGG 2 0.3399 Tier II
12 NC_000071.6 5 149578900 + Wdr95 NNNTATTTCAGATTAGAGAG NGG 2 0.2955 Tier II
13 NC_000081.6 15 80627248 - Grap2 NNNTATATCAGATTAGAGAG NGG 2 0.2727 Tier II
14 NC_000067.6 1 163309705 - Prrx1 NNNGAGCTCAGATTAGAGAC NGG 2 0.2449 Tier II
15 NC_000069.6 3 65064457 - Kcnab1 NNNAATCTCAGCTTAGAGAG NGG 2 0.2368 Tier II
16 NC_000069.6 3 68580262 + Schip1 NNNGATCTCAGGGTAGAGAG NGG 2 0.1884 Tier II
17 NC_000069.6 3 68580262 + Iqschfp NNNGATCTCAGGGTAGAGAG NGG 2 0.1884 Tier II
18 NC_000070.6 4 117960675 - St3gal3 NNNGGTCTCAGATTAGCGAG NGG 2 0.1271 Tier II
19 NC_000072.6 6 135148279 - Hebp1 NNNGATCTCAGAATAGTGAG NGG 2 0.0923 Tier II
20 NC_000077.6 11 59175795 + Gjc2 NNNGATCTCAGCTCAGAGAG NGG 2 0.0752 Tier II
21 NC_000085.6 19 46692844 + Borcs7 NNNGATCTCAGAGCAGAGAG NGG 2 0.0745 Tier II
22 NC_000073.6 7 43783905 + Klk10 NNNGATCTAAGATTAGAGAG NTG 2 0.0334 Tier II
23 NC_000069.6 3 109650490 + Vav3 NNNGATCTGAGATGAGAGAG NGG 2 0.0 Tier II
24 NC_000075.6 9 84004563 + Gm46124 NNNGATCTCTAATTAGAGAG NGG 2 0.8824 Tier III
25 NC_000071.6 5 149578900 + Gm35763 NNNTATTTCAGATTAGAGAG NGG 2 0.2955 Tier III
26 NC_000086.7 X 5816359 + LOC115489152 NNNCATCTCAGATTAGAGGG NGG 2 0.1985 Tier III
27 NC_000080.6 14 21997277 + A330001L22Rik NNNGTTCTCAGTTTAGAGAG NGG 2 0.1212 Tier III
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Other clones with same target sequence:

(none)