Construct: sgRNA BRDN0001148071
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GCCGGCAAATGCCTCCACAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- FGFR2 (2263)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76182
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000010.11 |
10 |
121520116 |
- |
FGFR2 |
NNNGGCAAATGCCTCCACAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
171108110 |
+ |
FMO3 |
NNNGGCAAATGCTTCCACAG |
NAG |
2 |
0.1815 |
Tier I |
| 3 |
NC_000014.9 |
14 |
76190435 |
+ |
GPATCH2L |
NNNGGCAAATACCACCACAG |
NGG |
2 |
0.619 |
Tier II |
| 4 |
NC_000010.11 |
10 |
48970549 |
+ |
WDFY4 |
NNNGGCAGGTGCCTCCACAG |
NGG |
2 |
0.4889 |
Tier II |
| 5 |
NC_000019.10 |
19 |
11007466 |
- |
SMARCA4 |
NNNGGCAAATGCCACCACAC |
NGG |
2 |
0.2653 |
Tier II |
| 6 |
NC_000007.14 |
7 |
128050233 |
- |
SND1 |
NNNTGCAAATGCCTCCATAG |
NGG |
2 |
0.2338 |
Tier II |
| 7 |
NC_000006.12 |
6 |
37680254 |
- |
MDGA1 |
NNNGGCACAGGCCTCCACAG |
NGG |
2 |
0.2143 |
Tier II |
| 8 |
NC_000007.14 |
7 |
2104219 |
+ |
MAD1L1 |
NNNGGCAAACTCCTCCACAG |
NGG |
2 |
0.2051 |
Tier II |
| 9 |
NC_000002.12 |
2 |
229028626 |
+ |
PID1 |
NNNGGCAAAGGCATCCACAG |
NGG |
2 |
0.1923 |
Tier II |
| 10 |
NC_000016.10 |
16 |
5450617 |
+ |
RBFOX1 |
NNNTGCAAATGGCTCCACAG |
NGG |
2 |
0.1616 |
Tier II |
| 11 |
NC_000010.11 |
10 |
103809561 |
+ |
SH3PXD2A |
NNNGGCAAATGCCTCTACAG |
NAG |
2 |
0.0798 |
Tier II |
| 12 |
NC_000001.11 |
1 |
167762127 |
- |
MPZL1 |
NNNTGCAAATGCCTCCTCAG |
NGG |
2 |
0.0485 |
Tier II |
| 13 |
NC_000013.11 |
13 |
107809435 |
- |
NALF1 |
NNNGGCAAATGCCTTCACAT |
NGG |
2 |
0.0467 |
Tier II |
| 14 |
NC_000001.11 |
1 |
150804466 |
- |
CTSK |
NNNGACAAATGCCTCCACAG |
NTG |
2 |
0.0338 |
Tier II |
| 15 |
NC_000003.12 |
3 |
46932716 |
+ |
CCDC12 |
NNNGGCAAATGCGTCCTCAG |
NGG |
2 |
0.0182 |
Tier II |
| 16 |
NC_000001.11 |
1 |
228312640 |
+ |
OBSCN |
NNNGGCACATGCCTCCACAG |
NGT |
2 |
0.0069 |
Tier II |
| 17 |
NC_000005.10 |
5 |
94258395 |
- |
KIAA0825 |
NNNGGCAAATGCCTCCACAG |
NAC |
2 |
0.0 |
Tier II |
| 18 |
NC_000011.10 |
11 |
31861428 |
+ |
PAX6-AS1 |
NNNAGCAAGTGCCTCCACAG |
NGG |
2 |
0.6 |
Tier III |
| 19 |
NC_000024.10 |
Y |
21393206 |
+ |
PRORY |
NNNGGCAAATGCCACCACAC |
NGG |
2 |
0.2653 |
Tier III |
| 20 |
NC_000007.14 |
7 |
128050233 |
- |
LOC124901743 |
NNNTGCAAATGCCTCCATAG |
NGG |
2 |
0.2338 |
Tier III |
| 21 |
NC_000011.10 |
11 |
10546076 |
+ |
IRAG1-AS1 |
NNNGGCATATGCCTACACAG |
NGG |
2 |
0.0952 |
Tier III |
| 22 |
NC_000013.11 |
13 |
107809435 |
- |
NALF1-IT1 |
NNNGGCAAATGCCTTCACAT |
NGG |
2 |
0.0467 |
Tier III |
| 23 |
NC_000006.12 |
6 |
8723276 |
- |
LOC100506207 |
NNNGGCAAATGCCACCACAG |
NGC |
2 |
0.0138 |
Tier III |
| 24 |
NC_000001.11 |
1 |
50248830 |
- |
LOC105378711 |
NNNGGCAAATGCCTCCACAG |
NTC |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000082.6 |
16 |
8683588 |
+ |
Gm5767 |
NNNGGCAAATGGCTCCACAC |
NGG |
2 |
0.1905 |
Tier I |
| 2 |
NC_000067.6 |
1 |
162966985 |
- |
Fmo3 |
NNNGGCAAATGCTTCCACAG |
NAG |
2 |
0.1815 |
Tier I |
| 3 |
NC_000073.6 |
7 |
130201120 |
- |
Fgfr2 |
NNNTGCAAATGCCTCCACGG |
NGG |
2 |
0.1364 |
Tier I |
| 4 |
NC_000081.6 |
15 |
71942247 |
- |
Col22a1 |
NNNGGCAAACGCCTCCACAG |
NGG |
1 |
0.5333 |
Tier II |
| 5 |
NC_000086.7 |
X |
138298240 |
- |
Il1rapl2 |
NNNGGATAATGCCTCCACAG |
NGG |
2 |
0.4062 |
Tier II |
| 6 |
NC_000070.6 |
4 |
125043948 |
- |
Gnl2 |
NNNGGCAACTGCCTCCACTG |
NGG |
2 |
0.3077 |
Tier II |
| 7 |
NC_000068.7 |
2 |
169991788 |
+ |
Tshz2 |
NNNGGGAAATCCCTCCACAG |
NGG |
2 |
0.2143 |
Tier II |
| 8 |
NC_000071.6 |
5 |
13185399 |
+ |
Sema3a |
NNNGTCAAATGCCTCCACAT |
NGG |
2 |
0.21 |
Tier II |
| 9 |
NC_000080.6 |
14 |
20407669 |
+ |
Cfap70 |
NNNTGCAAATTCCTCCACAG |
NGG |
2 |
0.1399 |
Tier II |
| 10 |
NC_000077.6 |
11 |
94566128 |
- |
Chad |
NNNGGCAGATGCCTCCCCAG |
NGG |
2 |
0.1294 |
Tier II |
| 11 |
NC_000077.6 |
11 |
94566128 |
- |
Acsf2 |
NNNGGCAGATGCCTCCCCAG |
NGG |
2 |
0.1294 |
Tier II |
| 12 |
NC_000072.6 |
6 |
80607329 |
- |
Lrrtm4 |
NNNGGAAAATGCCTTCACAG |
NGG |
2 |
0.0619 |
Tier II |
| 13 |
NC_000070.6 |
4 |
53453321 |
- |
Slc44a1 |
NNNGGCAAATGCCTCTGCAG |
NGG |
2 |
0.0543 |
Tier II |
| 14 |
NC_000085.6 |
19 |
53243161 |
- |
Add3 |
NNNGGCATATGCCTCCACAG |
NTG |
2 |
0.0167 |
Tier II |
| 15 |
NC_000074.6 |
8 |
23657944 |
- |
Zmat4 |
NNNGGCAAATGACTCCACAG |
NGC |
2 |
0.0159 |
Tier II |
| 16 |
NC_000074.6 |
8 |
46514816 |
+ |
Acsl1 |
NNNGGCAAGTGCCTCCACAG |
NGC |
2 |
0.0148 |
Tier II |
| 17 |
NC_000071.6 |
5 |
28084164 |
+ |
Gm35223 |
NNNGGCAAATGGCTCCACAG |
NGA |
2 |
0.0309 |
Tier III |
| 18 |
NC_000080.6 |
14 |
13275875 |
- |
Gm5087 |
NNNGGCAAATGCCTCCACAG |
NCC |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
