Construct: sgRNA BRDN0001148074
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGTCTCCACAGATTTACACA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PAK2 (5062)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77568
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000003.12 |
3 |
196807788 |
+ |
PAK2 |
NNNCTCCACAGATTTACACA |
NGG |
0 |
1.0 |
Tier I |
2 |
NC_000011.10 |
11 |
108281122 |
- |
ATM |
NNNTTTCACAGATTTACACA |
NGG |
2 |
0.7429 |
Tier I |
3 |
NC_000005.10 |
5 |
80782100 |
- |
MSH3 |
NNNCTCCAAAGATATACACA |
NGG |
2 |
0.5306 |
Tier II |
4 |
NC_000014.9 |
14 |
39072405 |
+ |
SEC23A |
NNNCTCCAAAGATATACACA |
NGG |
2 |
0.5306 |
Tier II |
5 |
NC_000015.10 |
15 |
78587416 |
- |
CHRNA5 |
NNNCTCCCCAGATATACACA |
NGG |
2 |
0.2653 |
Tier II |
6 |
NC_000012.12 |
12 |
77023642 |
+ |
E2F7 |
NNNCACCACAGATTTACTCA |
NGG |
2 |
0.25 |
Tier II |
7 |
NC_000023.11 |
X |
132842950 |
+ |
HS6ST2 |
NNNCACCACACATTTACACA |
NGG |
2 |
0.2143 |
Tier II |
8 |
NC_000014.9 |
14 |
63422149 |
+ |
PPP2R5E |
NNNCTGCACAGTTTTACACA |
NGG |
2 |
0.1667 |
Tier II |
9 |
NC_000003.12 |
3 |
49564364 |
- |
BSN |
NNNCTCCACAGATTCACACT |
NGG |
2 |
0.1636 |
Tier II |
10 |
NC_000019.10 |
19 |
41405908 |
- |
BCKDHA |
NNNCTGCACAGATTTACACA |
NAG |
2 |
0.1296 |
Tier II |
11 |
NC_000001.11 |
1 |
239732099 |
+ |
CHRM3 |
NNNCTCCACAGATTTGCACT |
NGG |
2 |
0.1154 |
Tier II |
12 |
NC_000014.9 |
14 |
104770568 |
+ |
AKT1 |
NNNCCCCACAGATTGACACA |
NGG |
2 |
0.05 |
Tier II |
13 |
NC_000023.11 |
X |
8670124 |
- |
ANOS1 |
NNNCTCCACAGATTTACAGA |
NAG |
2 |
0.0324 |
Tier II |
14 |
NC_000009.12 |
9 |
134157174 |
- |
WDR5 |
NNNCTCCCCAGATTTCCACA |
NGG |
2 |
0.0 |
Tier II |
15 |
NC_000014.9 |
14 |
53380078 |
+ |
LOC105370504 |
NNNCTCCAAAGATTTACAAA |
NGG |
2 |
0.3673 |
Tier III |
16 |
NC_000003.12 |
3 |
66712563 |
- |
LOC105377142 |
NNNCTCCACATTTTTACACA |
NGG |
2 |
0.1282 |
Tier III |
17 |
NC_000003.12 |
3 |
64955914 |
- |
ADAMTS9-AS2 |
NNNCTCCACAGATTTTCACA |
NGT |
2 |
0.0 |
Tier III |
18 |
NC_000003.12 |
3 |
64955914 |
- |
LOC105377124 |
NNNCTCCACAGATTTTCACA |
NGT |
2 |
0.0 |
Tier III |
19 |
NC_000002.12 |
2 |
238454690 |
- |
LOC100419686 |
NNNCTCCACAGCTTTCCACA |
NGG |
2 |
0.0 |
Tier III |
20 |
NC_000002.12 |
2 |
238454690 |
- |
LOC107986003 |
NNNCTCCACAGCTTTCCACA |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000082.6 |
16 |
32039926 |
- |
Pak2 |
NNNCTCTGCAGATTTACACA |
NGG |
2 |
0.5958 |
Tier I |
2 |
NC_000079.6 |
13 |
59701324 |
- |
Spata31d1a |
NNNCTCCACAGAGCTACACA |
NGG |
2 |
0.0745 |
Tier I |
3 |
NC_000079.6 |
13 |
59718053 |
+ |
Spata31d1b |
NNNCTCCACAGAGCTACACA |
NGG |
2 |
0.0745 |
Tier I |
4 |
NC_000074.6 |
8 |
88124109 |
- |
Cnep1r1 |
NNNCTTCATAGATTTACACA |
NGG |
2 |
0.8125 |
Tier II |
5 |
NC_000068.7 |
2 |
5834278 |
+ |
Cdc123 |
NNNCTACACAGAATTACACA |
NGG |
2 |
0.6429 |
Tier II |
6 |
NC_000070.6 |
4 |
117374281 |
+ |
Rnf220 |
NNNCTCCACAGATATACACA |
NGG |
1 |
0.619 |
Tier II |
7 |
NC_000067.6 |
1 |
177165973 |
+ |
Akt3 |
NNNCACCACAGATTTACACA |
NGG |
1 |
0.5 |
Tier II |
8 |
NC_000084.6 |
18 |
46261074 |
+ |
Pggt1b |
NNNCTACACAGATTTAAACA |
NGG |
2 |
0.4333 |
Tier II |
9 |
NC_000072.6 |
6 |
37276231 |
- |
Dgki |
NNNCAGCACAGATTTACACA |
NGG |
2 |
0.25 |
Tier II |
10 |
NC_000085.6 |
19 |
5221953 |
- |
Pacs1 |
NNNCTCCACAGATACACACA |
NGG |
2 |
0.1688 |
Tier II |
11 |
NC_000076.6 |
10 |
108730211 |
+ |
Syt1 |
NNNCTCCACAGAATTACAGA |
NGG |
2 |
0.0865 |
Tier II |
12 |
NC_000086.7 |
X |
83733827 |
+ |
Dmd |
NNNCTTCACAGATTTAGACA |
NGG |
2 |
0.0546 |
Tier II |
13 |
NC_000083.6 |
17 |
28599230 |
+ |
Srpk1 |
NNNATCCACAGATTTACACA |
NTG |
2 |
0.0328 |
Tier II |
14 |
NC_000070.6 |
4 |
44342665 |
- |
Melk |
NNNCTCCACAGACTTACACA |
NTG |
2 |
0.0308 |
Tier II |
15 |
NC_000078.6 |
12 |
91232430 |
+ |
Cep128 |
NNNCTCCACAGATTTACAAA |
NGA |
2 |
0.0298 |
Tier II |
16 |
NC_000075.6 |
9 |
60351331 |
+ |
Thsd4 |
NNNCTACACAGATTTACACA |
NGC |
2 |
0.0206 |
Tier II |
17 |
NC_000068.7 |
2 |
14760467 |
- |
Cacnb2 |
NNNCTCCAAAGATTTACACA |
NGT |
2 |
0.0138 |
Tier II |
18 |
NC_000078.6 |
12 |
56799147 |
+ |
Slc25a21 |
NNNCTCCACAAATTTTCACA |
NGG |
2 |
0.0 |
Tier II |
19 |
NC_000069.6 |
3 |
116127059 |
+ |
Vcam1 |
NNNCTCCACACATGTACACA |
NGG |
2 |
0.0 |
Tier II |
20 |
NC_000081.6 |
15 |
65846315 |
+ |
Efr3a |
NNNCTCCACATATTTTCACA |
NGG |
2 |
0.0 |
Tier II |
21 |
NC_000070.6 |
4 |
58511173 |
+ |
Lpar1 |
NNNGTCCACAGATTTTCACA |
NGG |
2 |
0.0 |
Tier II |
22 |
NC_000085.6 |
19 |
15659469 |
+ |
Gm46665 |
NNNCTCTACACATTTACACA |
NGG |
2 |
0.3482 |
Tier III |
23 |
NC_000067.6 |
1 |
183783691 |
+ |
Gm34068 |
NNNCTCCACAGAGTAACACA |
NGG |
2 |
0.151 |
Tier III |
24 |
NC_000076.6 |
10 |
118753536 |
- |
Gm33439 |
NNNCTCCACCCATTTACACA |
NGG |
2 |
0.1429 |
Tier III |
25 |
NC_000085.6 |
19 |
42780209 |
+ |
Gm16244 |
NNNCCCCACAGATTTACAGA |
NGG |
2 |
0.125 |
Tier III |
26 |
NC_000067.6 |
1 |
42682731 |
- |
Pantr1 |
NNNCTCCACATATTTACACA |
NGA |
2 |
0.0267 |
Tier III |
27 |
NC_000068.7 |
2 |
14760467 |
- |
Gm13269 |
NNNCTCCAAAGATTTACACA |
NGT |
2 |
0.0138 |
Tier III |
28 |
NC_000070.6 |
4 |
63703353 |
- |
Gm42294 |
NNNCTCCAAAGATGTACACA |
NGG |
2 |
0.0 |
Tier III |
29 |
NC_000086.7 |
X |
21505045 |
- |
Gm9439 |
NNNCTCCACAGCTTTCCACA |
NGG |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)