Construct: sgRNA BRDN0001148074

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TGTCTCCACAGATTTACACA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PAK2 (5062)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77568

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000003.12 3 196807788 + PAK2 NNNCTCCACAGATTTACACA NGG 0 1.0 Tier I
2 NC_000011.10 11 108281122 - ATM NNNTTTCACAGATTTACACA NGG 2 0.7429 Tier I
3 NC_000005.10 5 80782100 - MSH3 NNNCTCCAAAGATATACACA NGG 2 0.5306 Tier II
4 NC_000014.9 14 39072405 + SEC23A NNNCTCCAAAGATATACACA NGG 2 0.5306 Tier II
5 NC_000015.10 15 78587416 - CHRNA5 NNNCTCCCCAGATATACACA NGG 2 0.2653 Tier II
6 NC_000012.12 12 77023642 + E2F7 NNNCACCACAGATTTACTCA NGG 2 0.25 Tier II
7 NC_000023.11 X 132842950 + HS6ST2 NNNCACCACACATTTACACA NGG 2 0.2143 Tier II
8 NC_000014.9 14 63422149 + PPP2R5E NNNCTGCACAGTTTTACACA NGG 2 0.1667 Tier II
9 NC_000003.12 3 49564364 - BSN NNNCTCCACAGATTCACACT NGG 2 0.1636 Tier II
10 NC_000019.10 19 41405908 - BCKDHA NNNCTGCACAGATTTACACA NAG 2 0.1296 Tier II
11 NC_000001.11 1 239732099 + CHRM3 NNNCTCCACAGATTTGCACT NGG 2 0.1154 Tier II
12 NC_000014.9 14 104770568 + AKT1 NNNCCCCACAGATTGACACA NGG 2 0.05 Tier II
13 NC_000023.11 X 8670124 - ANOS1 NNNCTCCACAGATTTACAGA NAG 2 0.0324 Tier II
14 NC_000009.12 9 134157174 - WDR5 NNNCTCCCCAGATTTCCACA NGG 2 0.0 Tier II
15 NC_000014.9 14 53380078 + LOC105370504 NNNCTCCAAAGATTTACAAA NGG 2 0.3673 Tier III
16 NC_000003.12 3 66712563 - LOC105377142 NNNCTCCACATTTTTACACA NGG 2 0.1282 Tier III
17 NC_000003.12 3 64955914 - ADAMTS9-AS2 NNNCTCCACAGATTTTCACA NGT 2 0.0 Tier III
18 NC_000003.12 3 64955914 - LOC105377124 NNNCTCCACAGATTTTCACA NGT 2 0.0 Tier III
19 NC_000002.12 2 238454690 - LOC100419686 NNNCTCCACAGCTTTCCACA NGG 2 0.0 Tier III
20 NC_000002.12 2 238454690 - LOC107986003 NNNCTCCACAGCTTTCCACA NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000082.6 16 32039926 - Pak2 NNNCTCTGCAGATTTACACA NGG 2 0.5958 Tier I
2 NC_000079.6 13 59701324 - Spata31d1a NNNCTCCACAGAGCTACACA NGG 2 0.0745 Tier I
3 NC_000079.6 13 59718053 + Spata31d1b NNNCTCCACAGAGCTACACA NGG 2 0.0745 Tier I
4 NC_000074.6 8 88124109 - Cnep1r1 NNNCTTCATAGATTTACACA NGG 2 0.8125 Tier II
5 NC_000068.7 2 5834278 + Cdc123 NNNCTACACAGAATTACACA NGG 2 0.6429 Tier II
6 NC_000070.6 4 117374281 + Rnf220 NNNCTCCACAGATATACACA NGG 1 0.619 Tier II
7 NC_000067.6 1 177165973 + Akt3 NNNCACCACAGATTTACACA NGG 1 0.5 Tier II
8 NC_000084.6 18 46261074 + Pggt1b NNNCTACACAGATTTAAACA NGG 2 0.4333 Tier II
9 NC_000072.6 6 37276231 - Dgki NNNCAGCACAGATTTACACA NGG 2 0.25 Tier II
10 NC_000085.6 19 5221953 - Pacs1 NNNCTCCACAGATACACACA NGG 2 0.1688 Tier II
11 NC_000076.6 10 108730211 + Syt1 NNNCTCCACAGAATTACAGA NGG 2 0.0865 Tier II
12 NC_000086.7 X 83733827 + Dmd NNNCTTCACAGATTTAGACA NGG 2 0.0546 Tier II
13 NC_000083.6 17 28599230 + Srpk1 NNNATCCACAGATTTACACA NTG 2 0.0328 Tier II
14 NC_000070.6 4 44342665 - Melk NNNCTCCACAGACTTACACA NTG 2 0.0308 Tier II
15 NC_000078.6 12 91232430 + Cep128 NNNCTCCACAGATTTACAAA NGA 2 0.0298 Tier II
16 NC_000075.6 9 60351331 + Thsd4 NNNCTACACAGATTTACACA NGC 2 0.0206 Tier II
17 NC_000068.7 2 14760467 - Cacnb2 NNNCTCCAAAGATTTACACA NGT 2 0.0138 Tier II
18 NC_000078.6 12 56799147 + Slc25a21 NNNCTCCACAAATTTTCACA NGG 2 0.0 Tier II
19 NC_000069.6 3 116127059 + Vcam1 NNNCTCCACACATGTACACA NGG 2 0.0 Tier II
20 NC_000081.6 15 65846315 + Efr3a NNNCTCCACATATTTTCACA NGG 2 0.0 Tier II
21 NC_000070.6 4 58511173 + Lpar1 NNNGTCCACAGATTTTCACA NGG 2 0.0 Tier II
22 NC_000085.6 19 15659469 + Gm46665 NNNCTCTACACATTTACACA NGG 2 0.3482 Tier III
23 NC_000067.6 1 183783691 + Gm34068 NNNCTCCACAGAGTAACACA NGG 2 0.151 Tier III
24 NC_000076.6 10 118753536 - Gm33439 NNNCTCCACCCATTTACACA NGG 2 0.1429 Tier III
25 NC_000085.6 19 42780209 + Gm16244 NNNCCCCACAGATTTACAGA NGG 2 0.125 Tier III
26 NC_000067.6 1 42682731 - Pantr1 NNNCTCCACATATTTACACA NGA 2 0.0267 Tier III
27 NC_000068.7 2 14760467 - Gm13269 NNNCTCCAAAGATTTACACA NGT 2 0.0138 Tier III
28 NC_000070.6 4 63703353 - Gm42294 NNNCTCCAAAGATGTACACA NGG 2 0.0 Tier III
29 NC_000086.7 X 21505045 - Gm9439 NNNCTCCACAGCTTTCCACA NGG 2 0.0 Tier III
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Other clones with same target sequence:

(none)