Construct: sgRNA BRDN0001148079
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTTAGCAGTCGCTTATGGAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAP3K9 (4293)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77307
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000014.9 |
14 |
70750005 |
- |
MAP3K9 |
NNNAGCAGTCGCTTATGGAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
193253029 |
- |
CDC73 |
NNNAGCAGTAACTTATGGAG |
NGG |
2 |
0.8667 |
Tier II |
| 3 |
NC_000022.11 |
22 |
41619446 |
- |
DESI1 |
NNNAGCAGTAGCTTATGGAA |
NGG |
2 |
0.8125 |
Tier II |
| 4 |
NC_000022.11 |
22 |
48624408 |
+ |
TAFA5 |
NNNAGCACTCACTTATGGAG |
NGG |
2 |
0.6154 |
Tier II |
| 5 |
NC_000003.12 |
3 |
173099823 |
+ |
SPATA16 |
NNNAGCATGCGCTTATGGAG |
NGG |
2 |
0.3869 |
Tier II |
| 6 |
NC_000020.11 |
20 |
38539235 |
+ |
RALGAPB |
NNNAGCAGTTGCTTAGGGAG |
NGG |
2 |
0.3258 |
Tier II |
| 7 |
NC_000019.10 |
19 |
57961650 |
+ |
C19orf18 |
NNNAGGAGTCGCTTGTGGAG |
NGG |
2 |
0.325 |
Tier II |
| 8 |
NC_000009.12 |
9 |
347596 |
+ |
DOCK8 |
NNNAGCAGTCGCTTATGGAA |
NAG |
2 |
0.2431 |
Tier II |
| 9 |
NC_000023.11 |
X |
27819031 |
- |
MAGEB10 |
NNNAGCAGTCTGTTATGGAG |
NGG |
2 |
0.1709 |
Tier II |
| 10 |
NC_000003.12 |
3 |
140559721 |
- |
CLSTN2 |
NNNAGCAGTGGCTTTTGGAG |
NGG |
2 |
0.0778 |
Tier II |
| 11 |
NC_000005.10 |
5 |
157374191 |
- |
CYFIP2 |
NNNAGCAGTGGCTTTTGGAG |
NGG |
2 |
0.0778 |
Tier II |
| 12 |
NC_000015.10 |
15 |
40230798 |
+ |
BUB1B-PAK6 |
NNNAGCAGTAGCTGATGGAG |
NGG |
2 |
0.0 |
Tier II |
| 13 |
NC_000005.10 |
5 |
157374191 |
- |
NIPAL4-DT |
NNNAGCAGTGGCTTTTGGAG |
NGG |
2 |
0.0778 |
Tier III |
| 14 |
NC_000015.10 |
15 |
40230798 |
+ |
LOC107984763 |
NNNAGCAGTAGCTGATGGAG |
NGG |
2 |
0.0 |
Tier III |
| 15 |
NC_000023.11 |
X |
115905111 |
+ |
DANT2 |
NNNAGCAGTCTCTGATGGAG |
NGG |
2 |
0.0 |
Tier III |
| 16 |
NC_000023.11 |
X |
115905111 |
+ |
DANT1 |
NNNAGCAGTCTCTGATGGAG |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000080.6 |
14 |
79103652 |
+ |
Vwa8 |
NNNAGCAGTCGCTCATGGAA |
NGG |
2 |
0.2679 |
Tier I |
| 2 |
NC_000073.6 |
7 |
37455352 |
- |
Zfp536 |
NNNAGCAGTAGCTTATAGAG |
NGG |
2 |
0.8089 |
Tier II |
| 3 |
NC_000070.6 |
4 |
62667902 |
- |
Rgs3 |
NNNAGCTGTAGCTTATGGAG |
NGG |
2 |
0.3792 |
Tier II |
| 4 |
NC_000077.6 |
11 |
32405127 |
+ |
Sh3pxd2b |
NNNAGCACTCCCTTATGGAG |
NGG |
2 |
0.2637 |
Tier II |
| 5 |
NC_000083.6 |
17 |
87351221 |
- |
Ttc7 |
NNNAGCAGTGGCTTGTGGAG |
NGG |
2 |
0.2528 |
Tier II |
| 6 |
NC_000085.6 |
19 |
17780227 |
- |
Pcsk5 |
NNNAGCAGTGGCTTATGGAG |
NTG |
2 |
0.0152 |
Tier II |
| 7 |
NC_000068.7 |
2 |
64929267 |
+ |
Grb14 |
NNNAGCAGTCGCTGCTGGAG |
NGG |
2 |
0.0 |
Tier II |
| 8 |
NC_000070.6 |
4 |
62667902 |
- |
Gm52741 |
NNNAGCTGTAGCTTATGGAG |
NGG |
2 |
0.3792 |
Tier III |
| 9 |
NC_000079.6 |
13 |
107580897 |
- |
Gm31920 |
NNNAGCAGTCACTTATGGAG |
NGA |
2 |
0.0694 |
Tier III |
Other clones with same target sequence:
(none)
