Construct: sgRNA BRDN0001148084
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTCACGGCACACATTGCAGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- DGKD (8527)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77259
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
233434845 |
- |
DGKD |
NNNACGGCACACATTGCAGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000005.10 |
5 |
177995600 |
- |
PROP1 |
NNNACAGCTCACATTGCAGT |
NGG |
2 |
0.6 |
Tier II |
| 3 |
NC_000005.10 |
5 |
158916916 |
- |
EBF1 |
NNNGCGGCACACATTGCAGA |
NGG |
2 |
0.3516 |
Tier II |
| 4 |
NC_000005.10 |
5 |
38273646 |
- |
EGFLAM |
NNNACTGCACACATTGCAGT |
NCG |
2 |
0.0714 |
Tier II |
| 5 |
NC_000013.11 |
13 |
113094420 |
+ |
MCF2L |
NNNACGGCACACATTTCAGG |
NGG |
2 |
0.0 |
Tier II |
| 6 |
NC_000011.10 |
11 |
61832831 |
- |
FADS2 |
NNNAGGGCACACAGTGCAGT |
NGG |
2 |
0.0 |
Tier II |
| 7 |
NC_000012.12 |
12 |
5299286 |
+ |
LOC105369617 |
NNNACTGCACACATTGCTGT |
NGG |
2 |
0.3333 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000067.6 |
1 |
87915568 |
- |
Dgkd |
NNNACGACACACATTGCAAT |
NGG |
2 |
0.7143 |
Tier I |
| 2 |
NC_000075.6 |
9 |
26753176 |
- |
B3gat1 |
NNNATGGTACACATTGCAGT |
NGG |
2 |
0.5568 |
Tier II |
| 3 |
NC_000070.6 |
4 |
41205829 |
- |
Ubap2 |
NNNACGGGACACATTGTAGT |
NGG |
2 |
0.3 |
Tier II |
| 4 |
NC_000074.6 |
8 |
82338412 |
+ |
Il15 |
NNNATGGCACACATTGCAGT |
NGA |
2 |
0.0442 |
Tier II |
| 5 |
NC_000082.6 |
16 |
94792103 |
- |
Kcnj6 |
NNNAGGGCACACATTGCAGT |
NTG |
2 |
0.0234 |
Tier II |
| 6 |
NC_000068.7 |
2 |
126474751 |
+ |
Atp8b4 |
NNNACGGGACACATTGCAGT |
NGC |
2 |
0.0143 |
Tier II |
Other clones with same target sequence:
(none)
