Construct: sgRNA BRDN0001148085

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TGAACATGCTGATTGGCGTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DGKB (1607)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76917

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000007.14 7 14685273 - DGKB NNNACATGCTGATTGGCGTG NGG 0 1.0 Tier I
2 NC_000010.11 10 86687192 - LDB3 NNNACATGCTGATGGGCGTG NTG 2 0.0 Tier I
3 NC_000001.11 1 28048311 - EYA3 NNNACATGCTGATTAGCGTA NGG 2 0.8824 Tier II
4 NC_000023.11 X 31581198 + DMD NNNACATGCTGATTGGCTTG NGG 1 0.6667 Tier II
5 NC_000007.14 7 31885590 - PDE1C NNNACATGTTGATTGGTGTG NGG 2 0.4083 Tier II
6 NC_000002.12 2 111815004 - ANAPC1 NNNACATGCTGATTGGTCTG NGG 2 0.2222 Tier II
7 NC_000002.12 2 87725351 + ANAPC1P4 NNNACATGCTGATTGGTCTG NGG 2 0.2222 Tier III
8 NC_000002.12 2 87725351 + NCAL1 NNNACATGCTGATTGGTCTG NGG 2 0.2222 Tier III
9 NC_000002.12 2 87726574 + ANAPC1P4 NNNACATGCTGATTGGTCTG NGG 2 0.2222 Tier III
10 NC_000002.12 2 87726574 + NCAL1 NNNACATGCTGATTGGTCTG NGG 2 0.2222 Tier III
11 NC_000006.12 6 156684290 - LOC124901444 NNNACATGCTGATTGGAGGG NGG 2 0.1167 Tier III
12 NC_000013.11 13 60221474 - LINC00434 NNNACATGCTGATTGGCTTG NGA 2 0.0463 Tier III
13 NC_000013.11 13 58655071 - LOC105370219 NNNACATGCTCATTGGCGTG NGA 2 0.0298 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000078.6 12 38136631 + Dgkb NNNACATGCTGATTGGCGTG NGG 0 1.0 Tier I
2 NC_000074.6 8 72380927 - Eps15l1 NNNACATGCTGAGTGACGTG NGG 2 0.2609 Tier I
3 NC_000078.6 12 103442844 + Ifi27l2a NNNACCTGCTGATTGGAGTG NGG 2 0.2121 Tier I
4 NC_000083.6 17 47416033 - 1700001C19Rik NNNACATGCAGATTGGCTTG NGG 2 0.5714 Tier II
5 NC_000072.6 6 54276325 + Chn2 NNNACATACTGATTGGAGTG NGG 2 0.4667 Tier II
6 NC_000073.6 7 65928771 + Pcsk6 NNNACATGGCGATTGGCGTG NGG 2 0.3302 Tier II
7 NC_000083.6 17 25630866 + Lmf1 NNNACATGCTGATTGGTATG NGG 2 0.3231 Tier II
8 NC_000077.6 11 60519573 + Myo15 NNNACCTGCTGATTGGCTTG NGG 2 0.303 Tier II
9 NC_000067.6 1 9115280 - Sntg1 NNNACATTCTGATTGGTGTG NGG 2 0.2917 Tier II
10 NC_000067.6 1 150884971 - Hmcn1 NNNACATTCTGATTGGTGTG NGG 2 0.2917 Tier II
11 NC_000069.6 3 31949223 - Kcnmb2 NNNACATTCTGATTGGTGTG NGG 2 0.2917 Tier II
12 NC_000072.6 6 76898737 + Ctnna2 NNNAGATGCTCATTGGCGTG NGG 2 0.2571 Tier II
13 NC_000084.6 18 63800467 - Wdr7 NNNCCATTCTGATTGGCGTG NGG 2 0.2206 Tier II
14 NC_000069.6 3 133021773 - Gstcd NNNACATGCTGATTGGTGTG NGA 2 0.0324 Tier II
15 NC_000081.6 15 64134665 + Asap1 NNNACATGCTGAGTGGGGTG NGG 2 0.0153 Tier II
16 NC_000080.6 14 47398214 - Dlgap5 NNNACATTCTGATTGCCGTG NGG 2 0.0 Tier II
17 NC_000078.6 12 113809871 + Igh NNNACATTCTGACTGGCGTG NGG 2 0.4934 Tier III
18 NC_000072.6 6 54276325 + 9130019P16Rik NNNACATACTGATTGGAGTG NGG 2 0.4667 Tier III
19 NC_000073.6 7 65928771 + Gm51486 NNNACATGGCGATTGGCGTG NGG 2 0.3302 Tier III
20 NC_000068.7 2 95551347 + Gm13794 NNNACATTCTGATTGGTGTG NGG 2 0.2917 Tier III
21 NC_000073.6 7 3794707 - Gm15925 NNNACATTCTGATTGGTGTG NGG 2 0.2917 Tier III
22 NC_000078.6 12 115108217 - Igh NNNACATTCTGATTGGTGTG NGG 2 0.2917 Tier III
23 NC_000078.6 12 115326604 - Igh NNNACATTCTGATTGGTGTG NGG 2 0.2917 Tier III
24 NC_000080.6 14 53363742 + Tcra NNNACATTCTGATTGGTGTG NGG 2 0.2917 Tier III
25 NC_000080.6 14 53363742 + Trav13n-4 NNNACATTCTGATTGGTGTG NGG 2 0.2917 Tier III
26 NC_000081.6 15 70505130 - Gm18282 NNNACATTCTGATTGGTGTG NGG 2 0.2917 Tier III
27 NC_000075.6 9 36679077 - 1700027I24Rik NNNCCATTCTGATTGGCGTG NGG 2 0.2206 Tier III
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Other clones with same target sequence:

(none)