Construct: sgRNA BRDN0001148090
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTATGTCAGCAACGTATCCA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- FER (2241)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76800
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000005.10 |
5 |
108798385 |
+ |
FER |
NNNTGTCAGCAACGTATCCA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000020.11 |
20 |
51492914 |
+ |
NFATC2 |
NNNGGTCAGCGACGTATCCA |
NGG |
2 |
0.4063 |
Tier II |
| 3 |
NC_000001.11 |
1 |
206454093 |
- |
SRGAP2 |
NNNTGACAGCATCGTATCCA |
NGG |
2 |
0.2889 |
Tier II |
| 4 |
NC_000009.12 |
9 |
19842192 |
+ |
SLC24A2 |
NNNTGTCAGCACCGCATCCA |
NGG |
2 |
0.0718 |
Tier II |
| 5 |
NC_000001.11 |
1 |
60114919 |
+ |
LINC02778 |
NNNTGTCAGCAAGGTCTCCA |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000070.6 |
4 |
136637648 |
- |
Lactbl1 |
NNNTGGCAGCAGCGTATCCA |
NGG |
2 |
0.4127 |
Tier I |
| 2 |
NC_000083.6 |
17 |
63896279 |
+ |
Fer |
NNNTGTCAGCAACGTGTCCA |
NGG |
1 |
0.1923 |
Tier I |
| 3 |
NC_000082.6 |
16 |
32826786 |
- |
Rubcn |
NNNTGTCAGCAACTTCTCCA |
NGG |
2 |
0.0 |
Tier I |
| 4 |
NC_000075.6 |
9 |
28497714 |
+ |
Opcml |
NNNTGTCAGCAGTGTATCCA |
NGG |
2 |
0.5056 |
Tier II |
| 5 |
NC_000076.6 |
10 |
42816646 |
- |
Sec63 |
NNNTGTCAGCAACATATTCA |
NGG |
2 |
0.4821 |
Tier II |
| 6 |
NC_000084.6 |
18 |
58193773 |
+ |
Fbn2 |
NNNTGTCAACAAGGTATCCA |
NGG |
2 |
0.0877 |
Tier II |
| 7 |
NC_000069.6 |
3 |
120729392 |
+ |
6530403H02Rik |
NNNTGTCCACAACGTATCCA |
NGG |
2 |
0.2755 |
Tier III |
Other clones with same target sequence:
(none)
