Construct: sgRNA BRDN0001148094
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TCTGGAGCAGTCCATCGCAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- LMTK2 (22853)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76175
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000007.14 |
7 |
98186919 |
- |
LMTK2 |
NNNGGAGCAGTCCATCGCAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000005.10 |
5 |
15839042 |
- |
FBXL7 |
NNNGAAGCAGTCCATAGCAG |
NGG |
2 |
0.8667 |
Tier II |
| 3 |
NC_000011.10 |
11 |
71160104 |
+ |
SHANK2 |
NNNGGTACAGTCCATCGCAG |
NGG |
2 |
0.7143 |
Tier II |
| 4 |
NC_000002.12 |
2 |
178018254 |
- |
PDE11A |
NNNGGAGTAGTCCATCGCAG |
NGC |
2 |
0.0194 |
Tier II |
| 5 |
NC_000001.11 |
1 |
151533901 |
- |
CGN |
NNNGGAGCAGTCCAGCCCAG |
NGG |
2 |
0.0118 |
Tier II |
| 6 |
NC_000002.12 |
2 |
178018254 |
- |
SDHDP5 |
NNNGGAGTAGTCCATCGCAG |
NGC |
2 |
0.0194 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
144166340 |
- |
Lmtk2 |
NNNGGAGCAGTCCATCTCAG |
NGG |
1 |
0.25 |
Tier I |
| 2 |
NC_000072.6 |
6 |
63323268 |
+ |
Grid2 |
NNNAGAGCAGTCCATCACAG |
NGG |
2 |
0.84 |
Tier II |
| 3 |
NC_000071.6 |
5 |
27175562 |
+ |
Dpp6 |
NNNGGTGCAGTCCATCACAG |
NGG |
2 |
0.6667 |
Tier II |
| 4 |
NC_000071.6 |
5 |
140952555 |
- |
Card11 |
NNNGGTGCAGTCCATCACAG |
NGG |
2 |
0.6667 |
Tier II |
| 5 |
NC_000074.6 |
8 |
11456179 |
- |
Rab20 |
NNNGGTGCAGTCCATCACAG |
NGG |
2 |
0.6667 |
Tier II |
| 6 |
NC_000082.6 |
16 |
6952148 |
+ |
Rbfox1 |
NNNGGTGCAGTCCATCACAG |
NGG |
2 |
0.6667 |
Tier II |
| 7 |
NC_000075.6 |
9 |
45713509 |
+ |
Dscaml1 |
NNNGGGTCAGTCCATCGCAG |
NGG |
2 |
0.4082 |
Tier II |
| 8 |
NC_000081.6 |
15 |
6608274 |
- |
Fyb |
NNNGGAGCAGTGCAACGCAG |
NGG |
2 |
0.2573 |
Tier II |
| 9 |
NC_000081.6 |
15 |
6608494 |
- |
Fyb |
NNNGGAGCAGTGCAACGCAG |
NGG |
2 |
0.2573 |
Tier II |
| 10 |
NC_000075.6 |
9 |
103279201 |
- |
1300017J02Rik |
NNNGGAACAGTCCATCTCAG |
NGG |
2 |
0.25 |
Tier II |
| 11 |
NC_000070.6 |
4 |
149413929 |
- |
Ube4b |
NNNGGAGCAGTTCATCTCAG |
NGG |
2 |
0.1346 |
Tier II |
| 12 |
NC_000078.6 |
12 |
112279182 |
+ |
Gm36894 |
NNNGGAGCAGTCCTTCACAG |
NGG |
2 |
0.4978 |
Tier III |
| 13 |
NC_000081.6 |
15 |
6608274 |
- |
Gm2245 |
NNNGGAGCAGTGCAACGCAG |
NGG |
2 |
0.2573 |
Tier III |
| 14 |
NC_000081.6 |
15 |
6608494 |
- |
Gm2245 |
NNNGGAGCAGTGCAACGCAG |
NGG |
2 |
0.2573 |
Tier III |
Other clones with same target sequence:
(none)
