Construct: sgRNA BRDN0001148096

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GGACTGAAGGATGAAACAGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RP2 (6102)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76282

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000023.11 X 46853520 + RP2 NNNCTGAAGGATGAAACAGT NGG 0 1.0 Tier I
2 NC_000013.11 13 101141790 + NALCN NNNCTGGAGGATGAAACAGT NGG 1 0.7059 Tier II
3 NC_000018.10 18 10756762 + PIEZO2 NNNATGAAGGATGAAACATT NGG 2 0.5614 Tier II
4 NC_000007.14 7 126882699 - GRM8 NNNCTGGAGGATGAAACATT NGG 2 0.4706 Tier II
5 NC_000006.12 6 151919714 + ESR1 NNNCTGAAGGAGAAAACAGT NGG 2 0.4615 Tier II
6 NC_000004.12 4 80937080 - CFAP299 NNNCTGAAGGAAGAAACAGA NGG 2 0.45 Tier II
7 NC_000015.10 15 71707108 + THSD4 NNNCTGAAGGGTGAAACAGA NGG 2 0.3656 Tier II
8 NC_000001.11 1 52794721 + ZYG11B NNNCTTAAGGATGAAACTGT NGG 2 0.3333 Tier II
9 NC_000008.11 8 33554002 - RNF122 NNNGTGAAGGATGAGACAGT NGG 2 0.325 Tier II
10 NC_000005.10 5 156440278 - SGCD NNNCTGATGGATGAAACATT NGG 2 0.2857 Tier II
11 NC_000004.12 4 24184695 - PPARGC1A NNNCTGAAGGATGAAATAGA NGG 2 0.2625 Tier II
12 NC_000001.11 1 26032605 + EXTL1 NNNCTGAAGAATGACACAGT NGG 2 0.2545 Tier II
13 NC_000007.14 7 107429540 - COG5 NNNCTGGAGGTTGAAACAGT NGG 2 0.2172 Tier II
14 NC_000001.11 1 161173427 + PPOX NNNCTGAAGGATGACACAAT NGG 2 0.1948 Tier II
15 NC_000001.11 1 161173427 + B4GALT3 NNNCTGAAGGATGACACAAT NGG 2 0.1948 Tier II
16 NC_000002.12 2 174977583 + CHN1 NNNCTGAAGGATGACACAAT NGG 2 0.1948 Tier II
17 NC_000015.10 15 65392721 + IGDCC4 NNNCTGAAGGATCAAACACT NGG 2 0.1887 Tier II
18 NC_000006.12 6 489433 + EXOC2 NNNCTGAAAGATGACACAGT NGG 2 0.1753 Tier II
19 NC_000004.12 4 6130793 + JAKMIP1 NNNCTGAAGGAAGAAGCAGT NGG 2 0.1538 Tier II
20 NC_000023.11 X 85986955 + CHM NNNCTGAAGGATGAAACAGC NGG 1 0.0909 Tier II
21 NC_000018.10 18 46380568 - ARK2C NNNCTGAAGGATGAAGCACT NGG 2 0.0862 Tier II
22 NC_000007.14 7 129705847 + NRF1 NNNCTGAAGGCTGAAACAGG NGG 2 0.0706 Tier II
23 NC_000008.11 8 64703545 + CYP7B1 NNNCTGAAGGATGAAACATT NGA 2 0.0463 Tier II
24 NC_000003.12 3 115860403 + LSAMP NNNCTGAAGGATGCTACAGT NGG 2 0.0429 Tier II
25 NC_000004.12 4 97940679 - STPG2 NNNCTGAAGGATGAAATAGC NGG 2 0.0424 Tier II
26 NC_000005.10 5 40701316 + PTGER4 NNNCTGAAGGATGAAATAGC NGG 2 0.0424 Tier II
27 NC_000007.14 7 87670351 - ABCB1 NNNCTGAAGGATGAAATAGC NGG 2 0.0424 Tier II
28 NC_000007.14 7 87670351 - RUNDC3B NNNCTGAAGGATGAAATAGC NGG 2 0.0424 Tier II
29 NC_000002.12 2 63782052 - WDPCP NNNTTGAAGGATGAAACAGT NTG 2 0.0312 Tier II
30 NC_000013.11 13 98327754 + FARP1 NNNCTGAAGGATGAGACAGT NTG 2 0.0253 Tier II
31 NC_000010.11 10 19509813 + MALRD1 NNNCTGAAGGATGAAAGGGT NGG 2 0.0235 Tier II
32 NC_000001.11 1 77363284 - AK5 NNNCTGAAGGAAGAACCAGT NGG 2 0.0 Tier II
33 NC_000023.11 X 53238921 - IQSEC2 NNNCTGAAGGATGAACCAGG NGG 2 0.0 Tier II
34 NC_000013.11 13 18960672 - PHF2P2 NNNCTGGAGGATGAGACAGT NGG 2 0.4588 Tier III
35 NC_000018.10 18 35712143 + LOC105372064 NNNATGAAGGATGAAAAAGT NGG 2 0.393 Tier III
36 NC_000001.11 1 38100923 - MIR3659HG NNNCTGAAGACTGAAACAGT NGG 2 0.3733 Tier III
37 NC_000023.11 X 76876013 + MIR325HG NNNCTGAAGAATGAAACAGT NAG 2 0.242 Tier III
38 NC_000010.11 10 3934845 + LINC02660 NNNCTGAAGGAGGAAACACT NGG 2 0.2241 Tier III
39 NC_000006.12 6 22516488 + LOC105374971 NNNCTGAAGGATGAAGCACT NGG 2 0.0862 Tier III
40 NC_000009.12 9 1840663 - LOC105375951 NNNCTGAAGAATGAAAGAGT NGG 2 0.0549 Tier III
41 NC_000003.12 3 115860403 + LOC124906269 NNNCTGAAGGATGCTACAGT NGG 2 0.0429 Tier III
42 NC_000006.12 6 153370909 + LOC105378066 NNNCTGAAGGATGAAATAGC NGG 2 0.0424 Tier III
43 NC_000008.11 8 88458649 + LOC105375630 NNNCTGAAGGATGAAATAGC NGG 2 0.0424 Tier III
44 NC_000011.10 11 67385402 - LOC100130987 NNNCTGGAGGATGAAACAGT NTG 2 0.0275 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000086.7 X 20376925 + Rp2 NNNCTGAAGGATGAAACAGT NGG 0 1.0 Tier I
2 NC_000077.6 11 62404014 - Ncor1 NNNCTGAAGTAAGAAACAGT NGG 2 0.65 Tier II
3 NC_000067.6 1 68257918 - Erbb4 NNNCTGAAGTATGAAACAAT NGG 2 0.5804 Tier II
4 NC_000075.6 9 23478729 - Bmper NNNCTGAAGAATGAAACTGT NGG 2 0.4667 Tier II
5 NC_000083.6 17 70551686 + Dlgap1 NNNCGGGAGGATGAAACAGT NGG 2 0.4518 Tier II
6 NC_000078.6 12 36389817 + Crppa NNNCTGAAGGATAAAATAGT NGG 2 0.4308 Tier II
7 NC_000071.6 5 76343793 + Nmu NNNCTGAAGAATGAAACACT NGG 2 0.4184 Tier II
8 NC_000077.6 11 4685335 + Ascc2 NNNCTGGAGGAGGAAACAGT NGG 2 0.3529 Tier II
9 NC_000086.7 X 7933322 + Hdac6 NNNCTGAAAGATGGAACAGT NGG 2 0.3 Tier II
10 NC_000072.6 6 55810418 - Itprid1 NNNCTGAAGGATGGAACAGA NGG 2 0.2625 Tier II
11 NC_000074.6 8 35602971 - Mfhas1 NNNCTGAAGGCTGAGACAGT NGG 2 0.26 Tier II
12 NC_000075.6 9 48350883 - Nxpe2 NNNCAGAAGGATGAAATAGT NGG 2 0.2333 Tier II
13 NC_000075.6 9 48350883 - Nxpe4 NNNCAGAAGGATGAAATAGT NGG 2 0.2333 Tier II
14 NC_000068.7 2 118285760 - Gpr176 NNNCTGGAGGATGACACAGT NGG 2 0.1925 Tier II
15 NC_000084.6 18 75434715 - Ctif NNNCTGAAGGCTGAAAAAGT NGG 2 0.1867 Tier II
16 NC_000070.6 4 147338584 - Zfp978 NNNCAGAAGGATGAAACAGT NAG 2 0.1296 Tier II
17 NC_000070.6 4 150484995 - Rere NNNCTGAAAGATGAAGCAGT NGG 2 0.1236 Tier II
18 NC_000074.6 8 118244885 - Cdh13 NNNCTGAAGGATGAAATAGT NAG 2 0.121 Tier II
19 NC_000078.6 12 102413953 + Lgmn NNNCAGAAGGATGAAGCAGT NGG 2 0.0962 Tier II
20 NC_000073.6 7 101493566 - Pde2a NNNCAGAAGGATGAAACAGG NGG 2 0.0882 Tier II
21 NC_000071.6 5 112767650 - Myo18b NNNCTGAAGGCTGAAGCAGT NGG 2 0.0769 Tier II
22 NC_000067.6 1 129566883 - Thsd7b NNNCTGAAGGATGAAACAAT NTG 2 0.0278 Tier II
23 NC_000070.6 4 138458214 + Camk2n1 NNNCTGTAGGATGAAACAGT NTG 2 0.017 Tier II
24 NC_000081.6 15 9162433 - Lmbrd2 NNNCTGAAGGATGAAAGAGT NAG 2 0.0153 Tier II
25 NC_000068.7 2 169849190 - Tshz2 NNNCTGAAGGAGGAAACAGT NGC 2 0.0111 Tier II
26 NC_000084.6 18 10166507 + Rock1 NNNCTGAAGGATGCAACAGT NGC 2 0.0048 Tier II
27 NC_000068.7 2 58297003 + Acvr1c NNNCTGAAGGATGAATCAGC NGG 2 0.0 Tier II
28 NC_000069.6 3 83944681 - Tmem131l NNNCTGAGGGATGAATCAGT NGG 2 0.0 Tier II
29 NC_000083.6 17 4192092 + Gm41516 NNNTTCAAGGATGAAACAGT NGG 2 0.5455 Tier III
30 NC_000080.6 14 90217636 + 4930474H20Rik NNNCTGAAGAATGAAATAGT NGG 2 0.4356 Tier III
31 NC_000071.6 5 76343793 + Gm34175 NNNCTGAAGAATGAAACACT NGG 2 0.4184 Tier III
32 NC_000070.6 4 140324719 + Gm33895 NNNCTGAAGGATGAAACTCT NGG 2 0.2241 Tier III
33 NC_000070.6 4 140324719 + Gm9867 NNNCTGAAGGATGAAACTCT NGG 2 0.2241 Tier III
34 NC_000076.6 10 24460617 - Gm15270 NNNCTGAAGGATGGCACAGT NGG 2 0.1273 Tier III
35 NC_000068.7 2 132514896 + 1700026D11Rik NNNCTGAAGGAAGAAACAGT NGC 2 0.0178 Tier III
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Other clones with same target sequence:

(none)