Construct: sgRNA BRDN0001148097
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GATAGTATTTATATCCCCTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAP3K3 (4215)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76016
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000017.11 |
17 |
63667004 |
- |
MAP3K3 |
NNNAGTATTTATATCCCCTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000011.10 |
11 |
124016502 |
- |
OR10G4 |
NNNAGTATTTATTTCCTCTG |
NGG |
2 |
0.14 |
Tier I |
| 3 |
NC_000005.10 |
5 |
170810448 |
- |
GABRP |
NNNAGTATCTATATCCCCTG |
NGG |
1 |
0.9231 |
Tier II |
| 4 |
NC_000014.9 |
14 |
33704632 |
+ |
NPAS3 |
NNNAGTGTTTATATCACCTG |
NGG |
2 |
0.7059 |
Tier II |
| 5 |
NC_000001.11 |
1 |
205913501 |
- |
SLC26A9 |
NNNAGTATCTAGATCCCCTG |
NGG |
2 |
0.4615 |
Tier II |
| 6 |
NC_000010.11 |
10 |
115879079 |
- |
ATRNL1 |
NNNAGTATGTGTATCCCCTG |
NGG |
2 |
0.4024 |
Tier II |
| 7 |
NC_000018.10 |
18 |
33431620 |
- |
CCDC178 |
NNNAGTATTTATATCACCTG |
NAG |
2 |
0.2593 |
Tier II |
| 8 |
NC_000015.10 |
15 |
99110581 |
+ |
SYNM |
NNNAGTATTTAAATCCCCTG |
NAG |
2 |
0.2074 |
Tier II |
| 9 |
NC_000010.11 |
10 |
21926749 |
- |
DNAJC1 |
NNNAGTATTTATACCCCTTG |
NGG |
2 |
0.1837 |
Tier II |
| 10 |
NC_000015.10 |
15 |
55715323 |
- |
PRTG |
NNNAGTATTTATATCCCTGG |
NGG |
2 |
0.1607 |
Tier II |
| 11 |
NC_000016.10 |
16 |
80752027 |
- |
CDYL2 |
NNNAGTATTTATATCTTCTG |
NGG |
2 |
0.1436 |
Tier II |
| 12 |
NC_000018.10 |
18 |
46026227 |
- |
PSTPIP2 |
NNNATTATTTATATCCACTG |
NGG |
2 |
0.14 |
Tier II |
| 13 |
NC_000003.12 |
3 |
170180118 |
+ |
PHC3 |
NNNAGTATTTATATTCTCTG |
NGG |
2 |
0.0311 |
Tier II |
| 14 |
NC_000013.11 |
13 |
28892193 |
+ |
MTUS2 |
NNNAGTATTTATATACCCTG |
NGT |
2 |
0.0036 |
Tier II |
| 15 |
NC_000005.10 |
5 |
21027983 |
- |
LOC105374678 |
NNNGGTATTTATATCTCCTG |
NGG |
2 |
0.1923 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000077.6 |
11 |
106123850 |
- |
Map3k3 |
NNNGGTATTTATATCCCCTG |
NAG |
2 |
0.162 |
Tier I |
| 2 |
NC_000071.6 |
5 |
48472909 |
- |
Kcnip4 |
NNNAGTAGTTATATCCCCTC |
NGG |
2 |
0.3143 |
Tier II |
| 3 |
NC_000072.6 |
6 |
89471957 |
- |
Chchd6 |
NNNAGTATTTATGTCCTCTG |
NGG |
2 |
0.3043 |
Tier II |
| 4 |
NC_000067.6 |
1 |
93450928 |
+ |
Hdlbp |
NNNAGTATTTGTATCCACTG |
NGG |
2 |
0.3033 |
Tier II |
| 5 |
NC_000068.7 |
2 |
18314696 |
- |
Dnajc1 |
NNNAGTATTTATACCCCTTG |
NGG |
2 |
0.1837 |
Tier II |
| 6 |
NC_000082.6 |
16 |
55819121 |
- |
Nfkbiz |
NNNAGGATTTATATCCCCAG |
NGG |
2 |
0.1633 |
Tier II |
| 7 |
NC_000078.6 |
12 |
116925982 |
+ |
Ptprn2 |
NNNAGGATTTATATCCCCGG |
NGG |
2 |
0.1429 |
Tier II |
| 8 |
NC_000069.6 |
3 |
37560716 |
- |
Spata5 |
NNNATTATTTATATCCACTG |
NGG |
2 |
0.14 |
Tier II |
| 9 |
NC_000082.6 |
16 |
5029464 |
- |
Glyr1 |
NNNAGTTTTTATTTCCCCTG |
NGG |
2 |
0.1313 |
Tier II |
| 10 |
NC_000073.6 |
7 |
100601945 |
+ |
Mrpl48 |
NNNAGTATTTATATACCATG |
NGG |
2 |
0.1197 |
Tier II |
| 11 |
NC_000075.6 |
9 |
92560235 |
+ |
Plod2 |
NNNAGTATTTATATTACCTG |
NGG |
2 |
0.0667 |
Tier II |
| 12 |
NC_000069.6 |
3 |
14538435 |
+ |
Lrrcc1 |
NNNAGTATTTATATCCACTG |
NTG |
2 |
0.0182 |
Tier II |
| 13 |
NC_000071.6 |
5 |
144515282 |
- |
Gm33765 |
NNNAGTAACTATATCCCCTG |
NGG |
2 |
0.7385 |
Tier III |
| 14 |
NC_000075.6 |
9 |
91930015 |
+ |
Gm31409 |
NNNAGTATTTGTATCCCTTG |
NGG |
2 |
0.4179 |
Tier III |
| 15 |
NC_000081.6 |
15 |
70002881 |
+ |
Gm19782 |
NNNAATATTTATATCTCCTG |
NGG |
2 |
0.2667 |
Tier III |
| 16 |
NC_000069.6 |
3 |
37560716 |
- |
Gm36412 |
NNNATTATTTATATCCACTG |
NGG |
2 |
0.14 |
Tier III |
| 17 |
NC_000069.6 |
3 |
21765786 |
+ |
7530428D23Rik |
NNNAGTATTTATATGCCCTC |
NGG |
2 |
0.0214 |
Tier III |
Other clones with same target sequence:
(none)
