Construct: sgRNA BRDN0001148102
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTCACCTGAGAAATTCCGTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CAMK2B (816)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77742
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000007.14 |
7 |
44240719 |
+ |
CAMK2B |
NNNACCTGAGAAATTCCGTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
185309080 |
+ |
IVNS1ABP |
NNNACGTGAGAAATTCCATG |
NGG |
2 |
0.3462 |
Tier I |
| 3 |
NC_000004.12 |
4 |
113513340 |
+ |
CAMK2D |
NNNACCTGAGAAATTCCTTG |
NAG |
2 |
0.1728 |
Tier I |
| 4 |
NC_000001.11 |
1 |
231971385 |
- |
DISC1 |
NNNATCTGAGAAATTCCATG |
NGG |
2 |
0.4406 |
Tier II |
| 5 |
NC_000001.11 |
1 |
226587018 |
+ |
STUM |
NNNAACTGAGAAATTCCATG |
NGG |
2 |
0.3956 |
Tier II |
| 6 |
NC_000010.11 |
10 |
11174009 |
+ |
CELF2 |
NNNTACTGAGAAATTCCGTG |
NGG |
2 |
0.3636 |
Tier II |
| 7 |
NC_000023.11 |
X |
44284679 |
+ |
EFHC2 |
NNNTCCTGAGAAATTCAGTG |
NGG |
2 |
0.297 |
Tier II |
| 8 |
NC_000008.11 |
8 |
64635691 |
- |
CYP7B1 |
NNNACCTGAGAAATTCCATG |
NAG |
2 |
0.1795 |
Tier II |
| 9 |
NC_000004.12 |
4 |
163936507 |
+ |
MARCHF1 |
NNNACCTGAGAAATTCAGAG |
NGG |
2 |
0.1333 |
Tier II |
| 10 |
NC_000008.11 |
8 |
109096316 |
- |
TRHR |
NNNACCTGAGAAATTCAGTG |
NAG |
2 |
0.121 |
Tier II |
| 11 |
NC_000001.11 |
1 |
231971385 |
- |
TSNAX-DISC1 |
NNNATCTGAGAAATTCCATG |
NGG |
2 |
0.4406 |
Tier III |
| 12 |
NC_000020.11 |
20 |
26104706 |
+ |
NCOR1P1 |
NNNACCTGAAAAATTCTGTG |
NGG |
2 |
0.4356 |
Tier III |
| 13 |
NC_000023.11 |
X |
44284679 |
+ |
TATDN2P1 |
NNNTCCTGAGAAATTCAGTG |
NGG |
2 |
0.297 |
Tier III |
| 14 |
NC_000023.11 |
X |
115353374 |
+ |
LOC107985681 |
NNNACCTGAGAAATTCAGTC |
NGG |
2 |
0.2 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000077.6 |
11 |
5988113 |
+ |
Camk2b |
NNNACCTGAGAAATTCCGTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000083.6 |
17 |
24050433 |
- |
Kctd5 |
NNNACCTGAGAAGTTCCTTG |
NGG |
2 |
0.4348 |
Tier II |
| 3 |
NC_000073.6 |
7 |
29912353 |
- |
Zfp383 |
NNNAGCTGAGAAATTCCTTG |
NGG |
2 |
0.4 |
Tier II |
| 4 |
NC_000071.6 |
5 |
38359223 |
- |
Slc2a9 |
NNNGCCTGAGAAATTCTGTG |
NGG |
2 |
0.2917 |
Tier II |
| 5 |
NC_000079.6 |
13 |
55133274 |
+ |
Zfp346 |
NNNACCTGAGAAATTCCTTC |
NGG |
2 |
0.2857 |
Tier II |
| 6 |
NC_000071.6 |
5 |
116227683 |
- |
Ccdc60 |
NNNACCTGAGAAATGCCGTG |
NTG |
2 |
0.0019 |
Tier II |
| 7 |
NC_000083.6 |
17 |
27496472 |
- |
Grm4 |
NNNACGTGAGAAAGTCCGTG |
NGG |
2 |
0.0 |
Tier II |
| 8 |
NC_000071.6 |
5 |
38359223 |
- |
Gm40290 |
NNNGCCTGAGAAATTCTGTG |
NGG |
2 |
0.2917 |
Tier III |
| 9 |
NC_000067.6 |
1 |
112874268 |
- |
Gm8204 |
NNNACCTGAGAAATTCTGAG |
NGG |
2 |
0.1333 |
Tier III |
| 10 |
NC_000083.6 |
17 |
44117766 |
+ |
Gm34917 |
NNNACCTGAGAAATTACGTG |
NGA |
2 |
0.0694 |
Tier III |
Other clones with same target sequence:
(none)
