Construct: sgRNA BRDN0001148103
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGATGTAAATCACCCATTCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- RPS6KA1 (6195)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75498
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
26551457 |
+ |
RPS6KA1 |
NNNTGTAAATCACCCATTCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000012.12 |
12 |
85071649 |
- |
LRRIQ1 |
NNNTATAAATCACCCATTCT |
NGG |
2 |
0.6067 |
Tier II |
| 3 |
NC_000023.11 |
X |
69842223 |
+ |
EDA |
NNNTGTAAATCACACATACG |
NGG |
2 |
0.2333 |
Tier II |
| 4 |
NC_000002.12 |
2 |
79897727 |
- |
CTNNA2 |
NNNTGTAAATTACCCATTCT |
NGG |
2 |
0.2154 |
Tier II |
| 5 |
NC_000002.12 |
2 |
98351568 |
+ |
CNGA3 |
NNNTGTAAATTACCCATTCT |
NGG |
2 |
0.2154 |
Tier II |
| 6 |
NC_000012.12 |
12 |
85205259 |
+ |
LRRIQ1 |
NNNTGTAAATTTCCCATTCG |
NGG |
2 |
0.1026 |
Tier II |
| 7 |
NC_000002.12 |
2 |
177643077 |
+ |
PDE11A |
NNNTGTAAATCACCCAGTCT |
NGG |
2 |
0.0824 |
Tier II |
| 8 |
NC_000008.11 |
8 |
19628190 |
- |
CSGALNACT1 |
NNNTGTAAATCACCCAGTCT |
NGG |
2 |
0.0824 |
Tier II |
| 9 |
NC_000004.12 |
4 |
26129011 |
+ |
RBPJ |
NNNTGTAAATTACCCAGTCG |
NGG |
2 |
0.0362 |
Tier II |
| 10 |
NC_000001.11 |
1 |
105980945 |
+ |
LINC01677 |
NNNTATAAATCACCCATTCT |
NGG |
2 |
0.6067 |
Tier III |
| 11 |
NC_000024.10 |
Y |
11976102 |
- |
MXRA5Y |
NNNTGGAAATCACCCAATCG |
NGG |
2 |
0.3048 |
Tier III |
| 12 |
NC_000006.12 |
6 |
93706837 |
+ |
TSG1 |
NNNTGTAAATTACCCATTCT |
NGG |
2 |
0.2154 |
Tier III |
| 13 |
NC_000022.11 |
22 |
27960603 |
- |
TTC28-AS1 |
NNNTTTAAATCACCCATTTG |
NGG |
2 |
0.1385 |
Tier III |
| 14 |
NC_000001.11 |
1 |
89479899 |
- |
LOC105378844 |
NNNTGTAAATCACCCAGTCT |
NGG |
2 |
0.0824 |
Tier III |
| 15 |
NC_000011.10 |
11 |
7715121 |
+ |
LOC105376533 |
NNNTGTAAATCACCCAGTCT |
NGG |
2 |
0.0824 |
Tier III |
| 16 |
NC_000012.12 |
12 |
85480159 |
- |
LINC02820 |
NNNTGTAAATCACCCAGTCT |
NGG |
2 |
0.0824 |
Tier III |
| 17 |
NC_000020.11 |
20 |
55810973 |
+ |
LOC105372677 |
NNNTGTAAATCACCCAGTCT |
NGG |
2 |
0.0824 |
Tier III |
| 18 |
NC_000021.9 |
21 |
28060047 |
+ |
LINC01697 |
NNNTGTAAATTACCCATTCG |
NAG |
2 |
0.0798 |
Tier III |
| 19 |
NC_000007.14 |
7 |
136370009 |
+ |
LOC105375523 |
NNNTGTAAATCAGGCATTCG |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000073.6 |
7 |
78426117 |
+ |
Ntrk3 |
NNNTGTAAACCACCCATTCA |
NGG |
2 |
0.5 |
Tier II |
| 2 |
NC_000069.6 |
3 |
67953270 |
- |
Iqcj |
NNNTGTAAGTCACCCATTCT |
NGG |
2 |
0.4667 |
Tier II |
| 3 |
NC_000069.6 |
3 |
67953270 |
- |
Iqschfp |
NNNTGTAAGTCACCCATTCT |
NGG |
2 |
0.4667 |
Tier II |
| 4 |
NC_000079.6 |
13 |
54310195 |
- |
Cplx2 |
NNNTGTAAATCACCCATCCT |
NGG |
2 |
0.3 |
Tier II |
| 5 |
NC_000069.6 |
3 |
23917155 |
- |
Naaladl2 |
NNNTGTAAAACTCCCATTCG |
NGG |
2 |
0.2857 |
Tier II |
| 6 |
NC_000079.6 |
13 |
59731289 |
- |
Spata31d1d |
NNNTGTAAAGCACCCATTCC |
NGG |
2 |
0.2143 |
Tier II |
| 7 |
NC_000073.6 |
7 |
124272489 |
- |
Hs3st4 |
NNNTGTAAATTACCCATTTG |
NGG |
2 |
0.142 |
Tier II |
| 8 |
NC_000074.6 |
8 |
119197140 |
- |
Cdh13 |
NNNTGTAAATCACCCTTACG |
NGG |
2 |
0.0 |
Tier II |
| 9 |
NC_000079.6 |
13 |
54310195 |
- |
LOC115488208 |
NNNTGTAAATCACCCATCCT |
NGG |
2 |
0.3 |
Tier III |
Other clones with same target sequence:
(none)
