Construct: sgRNA BRDN0001148104
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGGGACCACTCACATCCTTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- RPS6KA2 (6196)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75733
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000006.12 |
6 |
166432405 |
+ |
RPS6KA2 |
NNNGACCACTCACATCCTTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000002.12 |
2 |
109394976 |
- |
RANBP2 |
NNNGGCCACACACATCCTTG |
NGG |
2 |
0.6171 |
Tier II |
| 3 |
NC_000002.12 |
2 |
109394976 |
- |
SH3RF3 |
NNNGGCCACACACATCCTTG |
NGG |
2 |
0.6171 |
Tier II |
| 4 |
NC_000015.10 |
15 |
61197977 |
- |
RORA |
NNNGTCTACTCACATCCTTG |
NGG |
2 |
0.2955 |
Tier II |
| 5 |
NC_000016.10 |
16 |
57811030 |
- |
KIFC3 |
NNNGCCCACTCACTTCCTTG |
NGG |
2 |
0.2667 |
Tier II |
| 6 |
NC_000003.12 |
3 |
50160523 |
- |
SEMA3F |
NNNGACCACACACACCCTTG |
NGG |
2 |
0.2338 |
Tier II |
| 7 |
NC_000003.12 |
3 |
126137219 |
+ |
ALDH1L1 |
NNNCACCACTCACATCCCTG |
NGG |
2 |
0.2269 |
Tier II |
| 8 |
NC_000002.12 |
2 |
28084345 |
- |
BABAM2 |
NNNTACCACTCACATCCCTG |
NGG |
2 |
0.1558 |
Tier II |
| 9 |
NC_000016.10 |
16 |
81471619 |
+ |
CMIP |
NNNGTCCACTCTCATCCTTG |
NGG |
2 |
0.1212 |
Tier II |
| 10 |
NC_000002.12 |
2 |
29329283 |
+ |
ALK |
NNNGACCACTCACATCTTTG |
NAG |
2 |
0.121 |
Tier II |
| 11 |
NC_000011.10 |
11 |
36376701 |
- |
PRR5L |
NNNCACCACTCACCTCCTTG |
NGG |
2 |
0.1134 |
Tier II |
| 12 |
NC_000003.12 |
3 |
132514141 |
- |
DNAJC13 |
NNNGACCACTCACATCTTTG |
NGA |
2 |
0.0324 |
Tier II |
| 13 |
NC_000011.10 |
11 |
120772095 |
+ |
GRIK4 |
NNNGACCACTCAGAGCCTTG |
NGG |
2 |
0.0068 |
Tier II |
| 14 |
NC_000015.10 |
15 |
61197977 |
- |
LOC105370841 |
NNNGTCTACTCACATCCTTG |
NGG |
2 |
0.2955 |
Tier III |
| 15 |
NC_000016.10 |
16 |
57811030 |
- |
LOC388282 |
NNNGCCCACTCACTTCCTTG |
NGG |
2 |
0.2667 |
Tier III |
| 16 |
NC_000002.12 |
2 |
29329283 |
+ |
LOC101929386 |
NNNGACCACTCACATCTTTG |
NAG |
2 |
0.121 |
Tier III |
| 17 |
NC_000004.12 |
4 |
160016677 |
+ |
LOC107986324 |
NNNGACCACTCACATCATGG |
NGG |
2 |
0.1167 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000073.6 |
7 |
3652125 |
- |
Cnot3 |
NNNCACCACTCACATCCTTG |
NCG |
2 |
0.0567 |
Tier I |
| 2 |
NC_000074.6 |
8 |
9683169 |
+ |
Fam155a |
NNNGACAAATCACATCCTTG |
NGG |
2 |
0.6429 |
Tier II |
| 3 |
NC_000084.6 |
18 |
63322291 |
+ |
Piezo2 |
NNNGAGAACTCACATCCTTG |
NGG |
2 |
0.375 |
Tier II |
| 4 |
NC_000068.7 |
2 |
24624976 |
- |
Cacna1b |
NNNTACCACTCACATCCTTG |
NGG |
1 |
0.3636 |
Tier II |
| 5 |
NC_000068.7 |
2 |
4082094 |
+ |
Frmd4a |
NNNGAGCACGCACATCCTTG |
NGG |
2 |
0.25 |
Tier II |
| 6 |
NC_000067.6 |
1 |
13696587 |
- |
Xkr9 |
NNNTACCACTCACATCCATG |
NGG |
2 |
0.2424 |
Tier II |
| 7 |
NC_000074.6 |
8 |
10625446 |
+ |
Myo16 |
NNNGACCACCCACATCCTTG |
NGA |
2 |
0.037 |
Tier II |
| 8 |
NC_000085.6 |
19 |
25361829 |
- |
Kank1 |
NNNGAGCACTCACATCCTTG |
NGA |
2 |
0.0347 |
Tier II |
| 9 |
NC_000067.6 |
1 |
163931990 |
+ |
Scyl3 |
NNNGACCACTCACTTCCTTG |
NTG |
2 |
0.0208 |
Tier II |
| 10 |
NC_000081.6 |
15 |
26376664 |
+ |
Marchf11 |
NNNGACCACTCACATCTTTG |
NTG |
2 |
0.0182 |
Tier II |
| 11 |
NC_000082.6 |
16 |
24935966 |
- |
Lpp |
NNNGACCACTCACTTCCTTG |
NGT |
2 |
0.0086 |
Tier II |
| 12 |
NC_000073.6 |
7 |
34952711 |
+ |
Pepd |
NNNTACCACTCACATCCTTG |
NGT |
2 |
0.0059 |
Tier II |
| 13 |
NC_000074.6 |
8 |
83605741 |
+ |
Gm42029 |
NNNGCCCACTCACATCTTTG |
NGG |
2 |
0.2333 |
Tier III |
| 14 |
NC_000075.6 |
9 |
61277027 |
+ |
B930092H01Rik |
NNNGACCACTCAAAACCTTG |
NGG |
2 |
0.2227 |
Tier III |
| 15 |
NC_000077.6 |
11 |
22567627 |
+ |
Gm51939 |
NNNGACCACTCTCATCCTTG |
NGA |
2 |
0.0231 |
Tier III |
| 16 |
NC_000082.6 |
16 |
24935966 |
- |
Gm52245 |
NNNGACCACTCACTTCCTTG |
NGT |
2 |
0.0086 |
Tier III |
Other clones with same target sequence:
(none)
