Construct: sgRNA BRDN0001148106
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGAGGTTCAATAAAGTACGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TTN (7273)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76854
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
178718214 |
+ |
TTN |
NNNGGTTCAATAAAGTACGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000010.11 |
10 |
86735168 |
- |
LDB3 |
NNNGATTCAATAAAGGACGG |
NGG |
2 |
0.3 |
Tier II |
| 3 |
NC_000016.10 |
16 |
24893504 |
- |
SLC5A11 |
NNNGGTTCAATAAAGGAAGG |
NGG |
2 |
0.1864 |
Tier II |
| 4 |
NC_000013.11 |
13 |
77629771 |
+ |
SCEL |
NNNGGTTCAATAAAGTACAG |
NGA |
2 |
0.0496 |
Tier II |
| 5 |
NC_000010.11 |
10 |
35665891 |
- |
LOC124902411 |
NNNGGTTCAATAAATTATGG |
NGG |
2 |
0.0918 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
76885023 |
+ |
Ttn |
NNNGGTTCGATAAAGTAAGG |
NGG |
2 |
0.359 |
Tier I |
| 2 |
NC_000079.6 |
13 |
81694679 |
+ |
Polr3g |
NNNGGTTCAATAAAGTACGG |
NAA |
2 |
0.0 |
Tier I |
| 3 |
NC_000081.6 |
15 |
74587630 |
- |
Adgrb1 |
NNNGGCTGAATAAAGTACGG |
NGG |
2 |
0.5844 |
Tier II |
| 4 |
NC_000074.6 |
8 |
40442737 |
+ |
Zdhhc2 |
NNNGGTTCAATAAAGCACAG |
NGG |
2 |
0.4762 |
Tier II |
| 5 |
NC_000077.6 |
11 |
37148949 |
+ |
Tenm2 |
NNNGGTTCAATAAAGTAAAG |
NGG |
2 |
0.3846 |
Tier II |
| 6 |
NC_000081.6 |
15 |
18773953 |
- |
C030047K22Rik |
NNNGATTCAATAAAGTACTG |
NGG |
2 |
0.5778 |
Tier III |
| 7 |
NC_000080.6 |
14 |
104363994 |
- |
D130079A08Rik |
NNNGGTTCCACAAAGTACGG |
NGG |
2 |
0.381 |
Tier III |
Other clones with same target sequence:
(none)
