Construct: sgRNA BRDN0001148109
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGATGGTAAGAAAGCGATGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TRPM6 (140803)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77336
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000009.12 |
9 |
74812342 |
+ |
TRPM6 |
NNNTGGTAAGAAAGCGATGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000022.11 |
22 |
23750455 |
- |
ZNF70 |
NNNTGGGAAGAAAGCGAGGG |
NGG |
2 |
0.1961 |
Tier II |
| 3 |
NC_000012.12 |
12 |
41519546 |
- |
PDZRN4 |
NNNTAGTAAGAAAGAGATGG |
NGG |
2 |
0.1926 |
Tier II |
| 4 |
NC_000009.12 |
9 |
20879681 |
+ |
FOCAD |
NNNTGGTAAGAAAGAGATTG |
NGG |
2 |
0.1481 |
Tier II |
| 5 |
NC_000018.10 |
18 |
42861622 |
- |
RIT2 |
NNNTGGTCAGAAAGAGATGG |
NGG |
2 |
0.0952 |
Tier II |
| 6 |
NC_000007.14 |
7 |
79102441 |
- |
MAGI2 |
NNNTGGTAAGAAAGAGATGG |
NAG |
2 |
0.0576 |
Tier II |
| 7 |
NC_000001.11 |
1 |
70414181 |
+ |
CTH |
NNNTGGTAAGAAGGTGATGG |
NGG |
2 |
0.0435 |
Tier II |
| 8 |
NC_000005.10 |
5 |
60085630 |
+ |
PDE4D |
NNNAGGTAAGAAAGTGATGG |
NGG |
2 |
0.0317 |
Tier II |
| 9 |
NC_000001.11 |
1 |
215876892 |
+ |
USH2A |
NNNTGGTAAGAAAGGGATGG |
NAG |
2 |
0.013 |
Tier II |
| 10 |
NC_000013.11 |
13 |
95200990 |
- |
ABCC4 |
NNNTGGTAAGAAAGCGTTGG |
NGC |
2 |
0.003 |
Tier II |
| 11 |
NC_000006.12 |
6 |
20139043 |
- |
MBOAT1 |
NNNTGGTAAGAAAGCCATGA |
NGG |
2 |
0.0 |
Tier II |
| 12 |
NC_000018.10 |
18 |
55530397 |
+ |
TCF4 |
NNNTGGTAAGAAAGCTATGG |
NGG |
1 |
0.0 |
Tier II |
| 13 |
NC_000012.12 |
12 |
7773301 |
- |
NANOGNB |
NNNTGGTAAGAAAGTTATGG |
NGG |
2 |
0.0 |
Tier II |
| 14 |
NC_000001.11 |
1 |
60811998 |
+ |
LOC101926964 |
NNNTGGGAAGAAAGCCATGG |
NGG |
2 |
0.0 |
Tier III |
| 15 |
NC_000020.11 |
20 |
57461637 |
- |
LOC105372688 |
NNNTGGTAAGAAACCCATGG |
NGG |
2 |
0.0 |
Tier III |
| 16 |
NC_000005.10 |
5 |
93578113 |
+ |
NR2F1-AS1 |
NNNTGGTAAGAAAGATATGG |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000078.6 |
12 |
103167402 |
- |
Unc79 |
NNNTGGTAAGAAAGCAATGT |
NGG |
2 |
0.7 |
Tier II |
| 2 |
NC_000083.6 |
17 |
44876436 |
- |
Supt3 |
NNNGGGTAAGAAAGCAATGG |
NGG |
2 |
0.625 |
Tier II |
| 3 |
NC_000072.6 |
6 |
27849389 |
+ |
Grm8 |
NNNTAGTAGGAAAGCGATGG |
NGG |
2 |
0.5778 |
Tier II |
| 4 |
NC_000082.6 |
16 |
22264484 |
- |
Tra2b |
NNNAGGAAAGAAAGCGATGG |
NGG |
2 |
0.4167 |
Tier II |
| 5 |
NC_000068.7 |
2 |
65006348 |
+ |
Grb14 |
NNNTGGGAAGAAAGAGATGG |
NGG |
2 |
0.1307 |
Tier II |
| 6 |
NC_000070.6 |
4 |
45915162 |
- |
Ccdc180 |
NNNAGGTAAGAAAGAGATGG |
NGG |
2 |
0.1058 |
Tier II |
| 7 |
NC_000078.6 |
12 |
85870253 |
- |
Ttll5 |
NNNTGGTAAGAAAGTGATGA |
NGG |
2 |
0.0625 |
Tier II |
| 8 |
NC_000076.6 |
10 |
62768021 |
- |
Ccar1 |
NNNTGGTAGGAAAGTGATGG |
NGG |
2 |
0.0444 |
Tier II |
| 9 |
NC_000071.6 |
5 |
124263636 |
+ |
Mphosph9 |
NNNTGGAAAGAAAGGGATGG |
NGG |
2 |
0.0438 |
Tier II |
| 10 |
NC_000069.6 |
3 |
159620919 |
+ |
Rpe65 |
NNNTGTTAAGAAAGGGATGG |
NGG |
2 |
0.0333 |
Tier II |
| 11 |
NC_000072.6 |
6 |
134682500 |
- |
Borcs5 |
NNNTGGTCAGAAAGCGATGG |
NGA |
2 |
0.0298 |
Tier II |
| 12 |
NC_000067.6 |
1 |
100371001 |
- |
Cntnap5b |
NNNTGGTAAGAAAGAGATGG |
NGA |
2 |
0.0154 |
Tier II |
| 13 |
NC_000082.6 |
16 |
89865930 |
+ |
Tiam1 |
NNNTGGTAAGAAAGGGTTGG |
NGG |
2 |
0.0067 |
Tier II |
| 14 |
NC_000075.6 |
9 |
63360404 |
+ |
Map2k5 |
NNNTGGTAACAAAGCCATGG |
NGG |
2 |
0.0 |
Tier II |
| 15 |
NC_000076.6 |
10 |
125999624 |
+ |
Lrig3 |
NNNTGGTAAGAAAGCCATGG |
NGT |
2 |
0.0 |
Tier II |
| 16 |
NC_000077.6 |
11 |
102085623 |
+ |
Mpp2 |
NNNTGGTAAGAAAGCTATGG |
NGA |
2 |
0.0 |
Tier II |
| 17 |
NC_000084.6 |
18 |
69399520 |
- |
Tcf4 |
NNNTGGTAAGAAAGCTATGG |
NGG |
1 |
0.0 |
Tier II |
| 18 |
NC_000078.6 |
12 |
111581655 |
+ |
Mark3 |
NNNTGGTAAGCAAGCTATGG |
NGG |
2 |
0.0 |
Tier II |
| 19 |
NC_000075.6 |
9 |
6345824 |
+ |
Pdgfd |
NNNTGGTTAGAAAGCTATGG |
NGG |
2 |
0.0 |
Tier II |
| 20 |
NC_000079.6 |
13 |
89748026 |
+ |
Gm4117 |
NNNTGATAAGAAAGCAATGG |
NGG |
2 |
1.0 |
Tier III |
| 21 |
NC_000081.6 |
15 |
36352936 |
+ |
Gm33936 |
NNNGGGTAAGAAAGAGATGG |
NGG |
2 |
0.1389 |
Tier III |
| 22 |
NC_000078.6 |
12 |
85870253 |
- |
Gm51990 |
NNNTGGTAAGAAAGTGATGA |
NGG |
2 |
0.0625 |
Tier III |
Other clones with same target sequence:
(none)
