Construct: sgRNA BRDN0001148112

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AGGGCACAAGAACAGTGCAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NTRK1 (4914)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75965

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000001.11 1 156868126 - NTRK1 NNNGCACAAGAACAGTGCAG NGG 0 1.0 Tier I
2 NC_000020.11 20 49906581 - SPATA2 NNNGCGCAAGAGCAGTGCAG NGG 2 0.5159 Tier I
3 NC_000009.12 9 20156774 - SLC24A2 NNNGCACAAGAACAGTGCAA NGG 1 0.9375 Tier II
4 NC_000002.12 2 47468717 - MSH2 NNNGCTCAAGAACAGTACAG NGG 2 0.6667 Tier II
5 NC_000016.10 16 6664968 - RBFOX1 NNNACACAAGAACAGTGCAT NGG 2 0.63 Tier II
6 NC_000023.11 X 110717354 + CHRDL1 NNNGCACAGGAACAATGCAG NGG 2 0.6275 Tier II
7 NC_000008.11 8 1366620 + DLGAP2 NNNGCACAGGAACAGAGCAG NGG 2 0.6061 Tier II
8 NC_000004.12 4 86221903 - MAPK10 NNNGCACAAGAACAGTGCTG NGG 1 0.5385 Tier II
9 NC_000001.11 1 63998889 - ROR1 NNNCCACAAGAACAGTACAG NGG 2 0.4941 Tier II
10 NC_000014.9 14 65396539 + FUT8 NNNCCACAAGAACAGTACAG NGG 2 0.4941 Tier II
11 NC_000017.11 17 57300845 + MSI2 NNNCCACAAGAACAGTACAG NGG 2 0.4941 Tier II
12 NC_000021.9 21 6536800 - LOC102724652 NNNCCACAAGAACAGTACAG NGG 2 0.4941 Tier II
13 NC_000017.11 17 17715237 - RAI1 NNNGCACAGGGACAGTGCAG NGG 2 0.4333 Tier II
14 NC_000007.14 7 1022142 - CHLSN NNNGCTCACGAACAGTGCAG NGG 2 0.4082 Tier II
15 NC_000004.12 4 91308178 - CCSER1 NNNGGACATGAACAGTGCAG NGG 2 0.36 Tier II
16 NC_000015.10 15 53663033 - WDR72 NNNGCACAATCACAGTGCAG NGG 2 0.325 Tier II
17 NC_000012.12 12 102435137 + IGF1 NNNGCACAAGAAAAGTGCAT NGG 2 0.2692 Tier II
18 NC_000003.12 3 43585888 - ANO10 NNNGAACAAGAACAGGGCAG NGG 2 0.1978 Tier II
19 NC_000010.11 10 119436631 - GRK5 NNNGCACAAGAACAGTGCCA NGG 2 0.194 Tier II
20 NC_000001.11 1 205978735 - RAB7B NNNGCCCAAGCACAGTGCAG NGG 2 0.1818 Tier II
21 NC_000011.10 11 79213339 + TENM4 NNNGCAGAAGAACACTGCAG NGG 2 0.1283 Tier II
22 NC_000002.12 2 100135127 + AFF3 NNNGCACCAGAACAGTTCAG NGG 2 0.1071 Tier II
23 NC_000002.12 2 228026012 - SPHKAP NNNTCACAAGAACAGTGCAG NAG 2 0.0943 Tier II
24 NC_000008.11 8 108457655 + EMC2 NNNTCACAAGAACAGTGCAG NAG 2 0.0943 Tier II
25 NC_000020.11 20 3331620 + DNAAF9 NNNTCACAAGAACAGTCCAG NGG 2 0.0856 Tier II
26 NC_000006.12 6 119045598 + FAM184A NNNGCAGAAGAACAGTGGAG NGG 2 0.0627 Tier II
27 NC_000011.10 11 78043595 + KCTD14 NNNGCACAAGCACAGTGGAG NGG 2 0.0533 Tier II
28 NC_000011.10 11 78043595 + NDUFC2-KCTD14 NNNGCACAAGCACAGTGGAG NGG 2 0.0533 Tier II
29 NC_000010.11 10 122096782 - TACC2 NNNGGACAAGAACAGTGCAG NGA 2 0.0417 Tier II
30 NC_000010.11 10 100806369 - PAX2 NNNGCACAGGAACAGTGCAG NGC 2 0.0148 Tier II
31 NC_000021.9 21 6536800 - LOC102724701 NNNCCACAAGAACAGTACAG NGG 2 0.4941 Tier III
32 NC_000021.9 21 43145115 - LOC101928369 NNNCCACAAGAACAGTACAG NGG 2 0.4941 Tier III
33 NC_000017.11 17 17715237 - LOC124903943 NNNGCACAGGGACAGTGCAG NGG 2 0.4333 Tier III
34 NC_000006.12 6 21947822 + CASC15 NNNGCACCAGAACAGTGCAG NGG 1 0.4286 Tier III
35 NC_000004.12 4 91308178 - LOC124900733 NNNGGACATGAACAGTGCAG NGG 2 0.36 Tier III
36 NC_000007.14 7 116298351 - LOC105375463 NNNCCACAAGAACAGTGTAG NGG 2 0.3403 Tier III
37 NC_000018.10 18 59051978 - OACYLP NNNGCACAAGAACAGTGATG NGG 2 0.2899 Tier III
38 NC_000012.12 12 102435137 + LINC02456 NNNGCACAAGAAAAGTGCAT NGG 2 0.2692 Tier III
39 NC_000008.11 8 20945298 + LOC105379315 NNNGCAGAAGAAAAGTGCAG NGG 2 0.181 Tier III
40 NC_000003.12 3 127326250 - LINC02016 NNNGCACAAGGACACTGCAG NGG 2 0.1773 Tier III
41 NC_000010.11 10 73667890 + SYNPO2L-AS1 NNNGCAGAAGAACAGGGCAG NGG 2 0.1629 Tier III
42 NC_000004.12 4 108171944 + LEF1-AS1 NNNGCACAAGAACAGAGGAG NGG 2 0.1212 Tier III
43 NC_000008.11 8 143194637 + LOC107986906 NNNGCCCAAGACCAGTGCAG NGG 2 0.1196 Tier III
44 NC_000002.12 2 228026012 - LOC105373918 NNNTCACAAGAACAGTGCAG NAG 2 0.0943 Tier III
45 NC_000011.10 11 47391041 + SLC39A13-AS1 NNNGCCCAAGAACAGTGCAG NGA 2 0.0316 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000069.6 3 87788752 + Ntrk1 NNNGCACAGGAACAATGCAG NGG 2 0.6275 Tier I
2 NC_000081.6 15 30434640 - Ctnnd2 NNNGCAAAAGAACAGTGCAG NGG 1 0.75 Tier II
3 NC_000073.6 7 99177247 + Dgat2 NNNGCATAAGAACAGAGCAG NGG 2 0.7386 Tier II
4 NC_000072.6 6 121051344 + Mical3 NNNGCACAGGAACAGTGCAG NGG 1 0.6667 Tier II
5 NC_000072.6 6 142891031 + St8sia1 NNNGCACAAGAGCAGAGCAG NGG 2 0.6566 Tier II
6 NC_000077.6 11 29947762 + Eml6 NNNGCACAATAGCAGTGCAG NGG 2 0.5868 Tier II
7 NC_000086.7 X 139870625 - Morc4 NNNGCAAAGGAACAGTGCAG NGG 2 0.5 Tier II
8 NC_000084.6 18 42758795 + Ppp2r2b NNNGCTCAAGAACAGTGCAT NGG 2 0.5 Tier II
9 NC_000067.6 1 120039162 - Sctr NNNGCACAAGAACTGTACAG NGG 2 0.4978 Tier II
10 NC_000077.6 11 52857398 + Fstl4 NNNGCACAAGGGCAGTGCAG NGG 2 0.4694 Tier II
11 NC_000069.6 3 119400645 + Dpyd NNNGAAAAAGAACAGTGCAG NGG 2 0.4286 Tier II
12 NC_000075.6 9 70922143 - Lipc NNNGCCCAAGAACAGAGCAG NGG 2 0.4132 Tier II
13 NC_000080.6 14 93351249 - Pcdh9 NNNGCACAAGAACAGTGAAT NGG 2 0.3769 Tier II
14 NC_000078.6 12 82358025 - Sipa1l1 NNNGCACAGGAACAGTGAAG NGG 2 0.359 Tier II
15 NC_000069.6 3 65375613 - Kcnab1 NNNGCACATGAACAGTGAAG NGG 2 0.3231 Tier II
16 NC_000070.6 4 94908417 + Eqtn NNNGCCCAAGAACAGTGAAG NGG 2 0.2448 Tier II
17 NC_000074.6 8 129170081 - Ccdc7b NNNGCACAAAAACAGTGCAG NAG 2 0.242 Tier II
18 NC_000070.6 4 156193030 - Agrn NNNGCACAAGGACAGGGCAG NGG 2 0.225 Tier II
19 NC_000082.6 16 57160324 + Nit2 NNNCCACAAGAAAAGTGCAG NGG 2 0.2036 Tier II
20 NC_000080.6 14 86772518 - Diaph3 NNNTCACAAGAACAGTGCTG NGG 2 0.1958 Tier II
21 NC_000078.6 12 44865673 + Stxbp6 NNNGCACAGGAACAGTGGAG NGG 2 0.0889 Tier II
22 NC_000085.6 19 46065518 + Pprc1 NNNGTACAAGAAGAGTGCAG NGG 2 0.0868 Tier II
23 NC_000072.6 6 119931217 + Wnk1 NNNGCACAAGAACAGAGCAG NGA 2 0.0631 Tier II
24 NC_000081.6 15 6749363 - Rictor NNNGCACAAGAACAGAGCAG NGA 2 0.0631 Tier II
25 NC_000078.6 12 104036847 - Serpina12 NNNGCACAACAACAGTGCAG NCG 2 0.0429 Tier II
26 NC_000084.6 18 33175614 - Camk4 NNNGCACAAGAAGACTGCAG NGG 2 0.0372 Tier II
27 NC_000081.6 15 33577476 + Cpq NNNGCACAAGAACAGTGGAG NAG 2 0.0346 Tier II
28 NC_000071.6 5 120666869 + Rasal1 NNNGCACATGAACAGTGCAG NTG 2 0.0234 Tier II
29 NC_000072.6 6 56136233 + Pde1c NNNGCAGAAGAACAGTGCAG NTG 2 0.0183 Tier II
30 NC_000075.6 9 70922143 - Gm32017 NNNGCCCAAGAACAGAGCAG NGG 2 0.4132 Tier III
31 NC_000077.6 11 109215200 - Gm39440 NNNGCACAAGAACAGAGCAC NGG 2 0.3896 Tier III
32 NC_000069.6 3 58813015 - Mindy4b-ps NNNGCACAAGATCAGAGCAG NGG 2 0.303 Tier III
33 NC_000070.6 4 156193030 - Gm46878 NNNGCACAAGGACAGGGCAG NGG 2 0.225 Tier III
34 NC_000067.6 1 186547186 - A730004F24Rik NNNGAACAAGAACAGGGCAG NGG 2 0.1978 Tier III
35 NC_000074.6 8 66908981 + Gm39185 NNNGCACATGAACACTGCAG NGG 2 0.1636 Tier III
36 NC_000069.6 3 39298055 + Gm42214 NNNGCACAAGAAGAGTGAAG NGG 2 0.0734 Tier III
37 NC_000078.6 12 104036847 - Gm31271 NNNGCACAACAACAGTGCAG NCG 2 0.0429 Tier III
38 NC_000078.6 12 114423664 - Igh NNNGCACAAGAACAGTGGAG NAG 2 0.0346 Tier III
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Other clones with same target sequence:

(none)