Construct: sgRNA BRDN0001148113
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GTAGGGGCTAGTTTAGGATG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PHKA1 (5255)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77841
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000023.11 |
X |
72620840 |
+ |
PHKA1 |
NNNGGGGCTAGTTTAGGATG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000011.10 |
11 |
120392701 |
- |
ARHGEF12 |
NNNAGGACTAGTTTAGGATG |
NGG |
2 |
0.9 |
Tier II |
| 3 |
NC_000001.11 |
1 |
154604815 |
- |
ADAR |
NNNAGGGCTAGTTTAGGTTG |
NGG |
2 |
0.45 |
Tier II |
| 4 |
NC_000003.12 |
3 |
113338827 |
+ |
CFAP44 |
NNNGGGGCTTGTTTAGGGTG |
NGG |
2 |
0.3529 |
Tier II |
| 5 |
NC_000004.12 |
4 |
963316 |
+ |
DGKQ |
NNNGGGGCTGGGTTAGGATG |
NGG |
2 |
0.2778 |
Tier II |
| 6 |
NC_000009.12 |
9 |
127406298 |
+ |
SLC2A8 |
NNNGGGGCTGGGTTAGGATG |
NGG |
2 |
0.2778 |
Tier II |
| 7 |
NC_000003.12 |
3 |
9144024 |
- |
SRGAP3 |
NNNGGGGCTAGTTTAGGAGG |
NGG |
1 |
0.25 |
Tier II |
| 8 |
NC_000015.10 |
15 |
92460410 |
+ |
ST8SIA2 |
NNNTGGGCTAGTTTAGGAGG |
NGG |
2 |
0.0909 |
Tier II |
| 9 |
NC_000013.11 |
13 |
36170186 |
+ |
SOHLH2 |
NNNGGGGCCAGTTGAGGATG |
NGG |
2 |
0.0 |
Tier II |
| 10 |
NC_000013.11 |
13 |
36170186 |
+ |
CCDC169-SOHLH2 |
NNNGGGGCCAGTTGAGGATG |
NGG |
2 |
0.0 |
Tier II |
| 11 |
NC_000008.11 |
8 |
27506802 |
- |
EPHX2 |
NNNGGGGCTATTTTATGATG |
NGG |
2 |
0.0 |
Tier II |
| 12 |
NC_000009.12 |
9 |
127508617 |
- |
NIBAN2 |
NNNGGGGCTGGTTGAGGATG |
NGG |
2 |
0.0 |
Tier II |
| 13 |
NC_000006.12 |
6 |
76990882 |
- |
LOC105377862 |
NNNGGGACTTGTTTAGGATG |
NGG |
2 |
0.8824 |
Tier III |
| 14 |
NC_000002.12 |
2 |
90030711 |
- |
IGK |
NNNGGGGCTAGTTTAAGATT |
NGG |
2 |
0.7 |
Tier III |
| 15 |
NC_000003.12 |
3 |
113338827 |
+ |
SPICE1-CFAP44 |
NNNGGGGCTTGTTTAGGGTG |
NGG |
2 |
0.3529 |
Tier III |
| 16 |
NC_000004.12 |
4 |
117833662 |
+ |
LOC102723914 |
NNNGGGGCTTGTTTAGGGTG |
NGG |
2 |
0.3529 |
Tier III |
| 17 |
NC_000014.9 |
14 |
88145350 |
- |
LOC107984699 |
NNNGGGGCTTGTTTAGGGTG |
NGG |
2 |
0.3529 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000086.7 |
X |
102557211 |
+ |
Phka1 |
NNNGGAGCTAGTTTAGGATG |
NGG |
1 |
1.0 |
Tier I |
| 2 |
NC_000076.6 |
10 |
81661999 |
+ |
Gm10778 |
NNNTGGGCTATTTTAGGATG |
NGG |
2 |
0.1399 |
Tier I |
| 3 |
NC_000076.6 |
10 |
81696375 |
+ |
Gm51792 |
NNNTGGGCTATTTTAGGATG |
NGG |
2 |
0.1399 |
Tier I |
| 4 |
NC_000076.6 |
10 |
81718920 |
+ |
Zfp433 |
NNNTGGGCTATTTTAGGATG |
NGG |
2 |
0.1399 |
Tier I |
| 5 |
NC_000076.6 |
10 |
81821128 |
+ |
Gm4767 |
NNNTGGGCTATTTTAGGATG |
NGG |
2 |
0.1399 |
Tier I |
| 6 |
NC_000076.6 |
10 |
81855477 |
+ |
Gm51793 |
NNNTGGGCTATTTTAGGATG |
NGG |
2 |
0.1399 |
Tier I |
| 7 |
NC_000076.6 |
10 |
81878030 |
+ |
Gm32687 |
NNNTGGGCTATTTTAGGATG |
NGG |
2 |
0.1399 |
Tier I |
| 8 |
NC_000076.6 |
10 |
81988592 |
+ |
Gm19802 |
NNNTGGGCTATTTTAGGATG |
NGG |
2 |
0.1399 |
Tier I |
| 9 |
NC_000073.6 |
7 |
55999303 |
- |
Nipa1 |
NNNGGTGCAAGTTTAGGATG |
NGG |
2 |
0.619 |
Tier II |
| 10 |
NC_000073.6 |
7 |
126674002 |
+ |
Sult1a1 |
NNNGGGGCTAGGATAGGATG |
NGG |
2 |
0.3462 |
Tier II |
| 11 |
NC_000082.6 |
16 |
22135328 |
- |
Igf2bp2 |
NNNGAGGCTAGTGTAGGATG |
NGG |
2 |
0.2261 |
Tier II |
| 12 |
NC_000073.6 |
7 |
129775658 |
+ |
Gm33323 |
NNNGGGGCTGGTTTGGGATG |
NGG |
2 |
0.3611 |
Tier III |
| 13 |
NC_000074.6 |
8 |
121869920 |
- |
Gm26812 |
NNNGGGGCAAGTTTAGGAGG |
NGG |
2 |
0.2321 |
Tier III |
| 14 |
NC_000076.6 |
10 |
92173302 |
+ |
Gm20757 |
NNNGGGGCTAGTTTTGAATG |
NGG |
2 |
0.1867 |
Tier III |
| 15 |
NC_000075.6 |
9 |
67018550 |
+ |
Gm30639 |
NNNGGGGCTGGTGTAGGATG |
NGG |
2 |
0.1449 |
Tier III |
Other clones with same target sequence:
(none)
