Construct: sgRNA BRDN0001148115

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TTTATACTTTCAGTGTTTAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GK (2710)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76326

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000023.11 X 30700842 + GK NNNATACTTTCAGTGTTTAG NGG 0 1.0 Tier I
2 NC_000011.10 11 133316850 + OPCML NNNATATTTTCAGTGTTTAA NGG 2 0.7617 Tier II
3 NC_000020.11 20 43688043 - MYBL2 NNNATGCTTTCAGTATTTAG NGG 2 0.6723 Tier II
4 NC_000005.10 5 60675466 - DEPDC1B NNNATACCTGCAGTGTTTAG NGG 2 0.5 Tier II
5 NC_000001.11 1 33485348 + ZSCAN20 NNNATACTTTCAGTGTCTAT NGG 2 0.4941 Tier II
6 NC_000001.11 1 25356771 - TMEM50A NNNATAATTGCAGTGTTTAG NGG 2 0.375 Tier II
7 NC_000004.12 4 90300124 - CCSER1 NNNATACTTGAAGTGTTTAG NGG 2 0.375 Tier II
8 NC_000015.10 15 52346911 - MYO5A NNNAGACTTTCAGTGTTTTG NGG 2 0.3446 Tier II
9 NC_000018.10 18 36449228 + FHOD3 NNNATACTTTCTGTGTTTAA NGG 2 0.3125 Tier II
10 NC_000002.12 2 174821054 + CHN1 NNNATACCTTTAGTGTTTAG NGG 2 0.3077 Tier II
11 NC_000001.11 1 225632116 + ENAH NNNATACTTTCATTGTTTAG NGG 1 0.3 Tier II
12 NC_000007.14 7 72295064 + CALN1 NNNATACTTTCAGTGTATAC NGG 2 0.2286 Tier II
13 NC_000007.14 7 8510826 - NXPH1 NNNATACTTTCAGTGTAGAG NGG 2 0.1778 Tier II
14 NC_000009.12 9 15658168 + CCDC171 NNNATACTTGCAGTGTTGAG NGG 2 0.1667 Tier II
15 NC_000005.10 5 76301210 + SV2C NNNATACTTTCTGTGTTCAG NGG 2 0.1429 Tier II
16 NC_000012.12 12 116092756 + MED13L NNNATACTTTCAGTGTGTAT NGG 2 0.0824 Tier II
17 NC_000012.12 12 68211468 + IL26 NNNATACTTTCAGTTTTTTG NGG 2 0.0769 Tier II
18 NC_000003.12 3 22208031 - ZNF385D NNNCTACTTTCAGTTTTTAG NGG 2 0.0504 Tier II
19 NC_000023.11 X 32275584 + DMD NNNATACTTTCAGTGTTTAG NTG 1 0.039 Tier II
20 NC_000008.11 8 124110225 - FER1L6 NNNATACATTCAGTGTTTAG NGC 2 0.0178 Tier II
21 NC_000006.12 6 16159415 - MYLIP NNNATACTTACAGTGTTTAG NGT 2 0.0138 Tier II
22 NC_000002.12 2 132493965 - GPR39 NNNATACTTTCAGTCTTTAG NTG 2 0.0106 Tier II
23 NC_000003.12 3 89327212 + EPHA3 NNNATACTGTCAGTGTTTAG NGT 2 0.01 Tier II
24 NC_000002.12 2 201235276 + CASP8 NNNATACTTTCAGTGTTTCG NTG 2 0.0081 Tier II
25 NC_000005.10 5 146844656 - PPP2R2B NNNATACTTTCAGTGTTCAG NGT 2 0.0069 Tier II
26 NC_000009.12 9 127109417 + RALGPS1 NNNATACATTCAGGGTTTAG NGG 2 0.0 Tier II
27 NC_000009.12 9 127109417 + ANGPTL2 NNNATACATTCAGGGTTTAG NGG 2 0.0 Tier II
28 NC_000023.11 X 30700842 + GK-AS1 NNNATACTTTCAGTGTTTAG NGG 0 1.0 Tier III
29 NC_000002.12 2 33908902 + LINC01317 NNNATACATTCAGTGATTAG NGG 2 0.7273 Tier III
30 NC_000003.12 3 20268959 - RNU6-822P NNNATATTTTAAGTGTTTAG NGG 2 0.6094 Tier III
31 NC_000006.12 6 54790954 + LOC107986606 NNNATACTTTCAGAATTTAG NGG 2 0.5826 Tier III
32 NC_000003.12 3 72044010 - LINC00877 NNNACACTTTCAGTGTTTTG NGG 2 0.5385 Tier III
33 NC_000016.10 16 60381061 - LOC101927605 NNNATACTTTAAGAGTTTAG NGG 2 0.4643 Tier III
34 NC_000002.12 2 21318812 + LOC105374318 NNNTTGCTTTCAGTGTTTAG NGG 2 0.4545 Tier III
35 NC_000007.14 7 44994306 + LOC102723334 NNNAAATTTTCAGTGTTTAG NGG 2 0.4063 Tier III
36 NC_000002.12 2 40808168 - LOC105374497 NNNATAATTTCAGTGTTTTG NGG 2 0.4038 Tier III
37 NC_000012.12 12 74180776 + LINC02882 NNNATATTTTCAGTGTTTGG NGG 2 0.3047 Tier III
38 NC_000021.9 21 9081755 + TEKT4P2 NNNCTATTTTCAGTGTTTAG NGG 2 0.2868 Tier III
39 NT_113888.1 14 66997 + MAFIP NNNCTATTTTCAGTGTTTAG NGG 2 0.2868 Tier III
40 NT_113888.1 14 66997 + LOC128966559 NNNCTATTTTCAGTGTTTAG NGG 2 0.2868 Tier III
41 NT_113889.1 52185 + LOC389834 NNNCTATTTTCAGTGTTTAG NGG 2 0.2868 Tier III
42 NT_113889.1 52185 + LOC100233156 NNNCTATTTTCAGTGTTTAG NGG 2 0.2868 Tier III
43 NC_000011.10 11 124218381 + LOC102724410 NNNCTACTTTCAGTGTTTTG NGG 2 0.19 Tier III
44 NC_000005.10 5 4822690 - LOC107986400 NNNATACTTTTAGTGTTTTG NGG 2 0.1657 Tier III
45 NC_000005.10 5 76301210 + LOC105379042 NNNATACTTTCTGTGTTCAG NGG 2 0.1429 Tier III
46 NC_000002.12 2 198592840 + LOC105373831 NNNAAACTTTCAGTGTTTAG NGA 2 0.0347 Tier III
47 NC_000023.11 X 146922952 - LOC101928832 NNNATCCTTTCAGTGTTTAG NGA 2 0.0316 Tier III
48 NC_000008.11 8 124110225 - FER1L6-AS2 NNNATACATTCAGTGTTTAG NGC 2 0.0178 Tier III
49 NC_000007.14 7 89446588 + LOC105375387 NNNATATTTTCAGTGTTTAG NGT 2 0.0131 Tier III
50 NC_000004.12 4 73342517 - ANKRD17-DT NNNATACTTTCAGTGTTTAG NAC 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000072.6 6 34726754 + Cald1 NNNACACTTTCAATGTTTAG NGG 2 0.9231 Tier II
2 NC_000079.6 13 12507864 + Edaradd NNNATATTTTCAGTGTTTAA NGG 2 0.7617 Tier II
3 NC_000077.6 11 84176913 + Acaca NNNATACATTCAGTGTCTAG NGG 2 0.5647 Tier II
4 NC_000068.7 2 112567073 - Aven NNNATACTTTCAGTATATAG NGG 2 0.502 Tier II
5 NC_000080.6 14 103215969 - Mycbp2 NNNATCCTTTCAGTATTTAG NGG 2 0.4278 Tier II
6 NC_000073.6 7 97719913 - Clns1a NNNATACTTTCAATGTTGAG NGG 2 0.3077 Tier II
7 NC_000078.6 12 38576700 - Dgkb NNNATTCTTTCACTGTTTAG NGG 2 0.3008 Tier II
8 NC_000067.6 1 61694021 - Pard3b NNNATACTTTCATTGATTAG NGG 2 0.2727 Tier II
9 NC_000071.6 5 130968563 + Galnt17 NNNATATTTTTAGTGTTTAG NGG 2 0.25 Tier II
10 NC_000086.7 X 92527276 - Mageb18 NNNATACTTTGAATGTTTAG NGG 2 0.2308 Tier II
11 NC_000069.6 3 129837374 - Cfi NNNATACTTTCAGTGTATAC NGG 2 0.2286 Tier II
12 NC_000068.7 2 76640655 - Prkra NNNAGACTTTCTGTGTTTAG NGG 2 0.2133 Tier II
13 NC_000075.6 9 110486854 + Kif9 NNNATAATTTCAGTGTTTAG NAG 2 0.1944 Tier II
14 NC_000076.6 10 120970143 - Lemd3 NNNAGACTTTCAGTCTTTAG NGG 2 0.1745 Tier II
15 NC_000083.6 17 67197589 - Ptprm NNNATAGTTTCAGTGTTTAG NAG 2 0.122 Tier II
16 NC_000086.7 X 6578403 + Shroom4 NNNATAGTTTCAGTGTTTAG NAG 2 0.122 Tier II
17 NC_000070.6 4 106959744 - Ssbp3 NNNTTACTTTCAGTGTGTAG NGG 2 0.0749 Tier II
18 NC_000071.6 5 120676128 - Rasal1 NNNATACTTTAAGTGTTTAG NTG 2 0.0292 Tier II
19 NC_000079.6 13 109001939 - Pde4d NNNATACTTTCAGTGTCTAG NTG 2 0.0275 Tier II
20 NC_000072.6 6 34726754 + Gm38787 NNNACACTTTCAATGTTTAG NGG 2 0.9231 Tier III
21 NC_000076.6 10 129229606 - Gm6538 NNNATAATCTCAGTGTTTAG NGG 2 0.6923 Tier III
22 NC_000072.6 6 55615429 + Gm34441 NNNATACTATCAGTGTTCAG NGG 2 0.398 Tier III
23 NC_000078.6 12 38576700 - 4930428E07Rik NNNATTCTTTCACTGTTTAG NGG 2 0.3008 Tier III
24 NC_000076.6 10 24570030 - Gm15270 NNNATACTTCCAGTGTATAG NGG 2 0.2844 Tier III
25 NC_000079.6 13 84658393 + Gm34196 NNNATCCTGTCAGTGTTTAG NGG 2 0.2814 Tier III
26 NC_000080.6 14 106453938 + 1700128A07Rik NNNACACTTTCAGTTTTTAG NGG 2 0.1429 Tier III
27 NC_000084.6 18 80574411 + Gm41790 NNNATACTTTCAGTGTTTGG NAG 2 0.0972 Tier III
28 NC_000081.6 15 18775286 + C030047K22Rik NNNATATTTTCAGTGTTTAG NGC 2 0.0181 Tier III
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Other clones with same target sequence:

(none)