Construct: sgRNA BRDN0001148116
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CAGCTGTTAGGCGATCCATG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAPK4 (5596)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77220
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000018.10 |
18 |
50726002 |
- |
MAPK4 |
NNNCTGTTAGGCGATCCATG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000015.10 |
15 |
52061339 |
- |
MAPK6 |
NNNCTGTTAACCGATCCATG |
NGG |
2 |
0.4 |
Tier I |
| 3 |
NC_000007.14 |
7 |
76053291 |
- |
MDH2 |
NNNCTGTTAGGCGTTCCATG |
NGT |
2 |
0.0086 |
Tier II |
| 4 |
NC_000003.12 |
3 |
156783761 |
- |
LINC00886 |
NNNCTGTTTTGCGATCCATG |
NGG |
2 |
0.4875 |
Tier III |
| 5 |
NC_000015.10 |
15 |
52061339 |
- |
LOC112268150 |
NNNCTGTTAACCGATCCATG |
NGG |
2 |
0.4 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000084.6 |
18 |
73934059 |
+ |
Mapk4 |
NNNCTGTTAAGCGGTCCATG |
NGG |
2 |
0.4356 |
Tier I |
| 2 |
NC_000076.6 |
10 |
34072208 |
- |
Trappc3l |
NNNGTCTTAGGCGATCCATG |
NGG |
2 |
0.3409 |
Tier II |
| 3 |
NC_000075.6 |
9 |
14757159 |
+ |
1700012B09Rik |
NNNCTGTTAGGCAATCCCTG |
NGG |
2 |
0.1758 |
Tier II |
| 4 |
NC_000084.6 |
18 |
67947836 |
+ |
Ldlrad4 |
NNNCTGTTAGGCGATCCCTG |
NGA |
2 |
0.0132 |
Tier II |
| 5 |
NC_000079.6 |
13 |
52839707 |
- |
Auh |
NNNCTGTTAGCCGATCCATG |
NGT |
2 |
0.0069 |
Tier II |
| 6 |
NC_000079.6 |
13 |
52839707 |
- |
Gm33315 |
NNNCTGTTAGCCGATCCATG |
NGT |
2 |
0.0069 |
Tier III |
Other clones with same target sequence:
(none)
