Construct: sgRNA BRDN0001148117
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AAGTAAATGTTCGGTCACAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CSNK1G1 (53944)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77438
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000015.10 |
15 |
64216611 |
+ |
CSNK1G1 |
NNNTAAATGTTCGGTCACAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000003.12 |
3 |
76956100 |
+ |
ROBO2 |
NNNTAAATGTTCAGTAACAG |
NGG |
2 |
0.9231 |
Tier II |
| 3 |
NC_000005.10 |
5 |
42005945 |
- |
FBXO4 |
NNNTAAATGTTAGGTCACAG |
NGG |
1 |
0.7143 |
Tier II |
| 4 |
NC_000023.11 |
X |
141875484 |
+ |
MAGEC3 |
NNNTAAATCTTCGGTGACAG |
NGG |
2 |
0.0828 |
Tier II |
| 5 |
NC_000009.12 |
9 |
41961161 |
+ |
CNTNAP3B |
NNNTAAATATTCGGTCACAG |
NGT |
2 |
0.0104 |
Tier II |
| 6 |
NC_000010.11 |
10 |
120837119 |
+ |
WDR11-DT |
NNNTAAATGTTAGGTCATAG |
NGG |
2 |
0.4592 |
Tier III |
| 7 |
NC_000003.12 |
3 |
148287310 |
- |
LINC02046 |
NNNTAAATGTTGGGCCACAG |
NGG |
2 |
0.1212 |
Tier III |
| 8 |
NC_000014.9 |
14 |
48823923 |
+ |
LOC105378178 |
NNNAAAATGTTCGGGCACAG |
NGG |
2 |
0.0238 |
Tier III |
| 9 |
NC_000002.12 |
2 |
56053281 |
- |
LOC105374690 |
NNNTAAATGTTCGGTTACAG |
NGA |
2 |
0.0214 |
Tier III |
| 10 |
NC_000009.12 |
9 |
61104847 |
+ |
LOC101930090 |
NNNTAAATATTCGGTCACAG |
NGT |
2 |
0.0104 |
Tier III |
| 11 |
NC_000009.12 |
9 |
67226301 |
- |
CNTNAP3P2 |
NNNTAAATATTCGGTCACAG |
NGT |
2 |
0.0104 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000075.6 |
9 |
65999498 |
- |
Csnk1g1 |
NNNTAAACGTTCGGTCACAG |
NGG |
1 |
1.0 |
Tier I |
| 2 |
NC_000080.6 |
14 |
12147725 |
+ |
Ptprg |
NNNTAAATGTCTGGTCACAG |
NGG |
2 |
0.359 |
Tier II |
| 3 |
NC_000075.6 |
9 |
109403261 |
- |
Fbxw22 |
NNNTAAATGTTTGCTCACAG |
NGG |
2 |
0.2308 |
Tier II |
| 4 |
NC_000075.6 |
9 |
109701466 |
- |
Fbxw18 |
NNNTAAATGTTTGCTCACAG |
NGG |
2 |
0.2308 |
Tier II |
| 5 |
NC_000086.7 |
X |
59259882 |
- |
Fgf13 |
NNNTAAATGTTTGGTCTCAG |
NGG |
2 |
0.0718 |
Tier II |
| 6 |
NC_000080.6 |
14 |
78288083 |
+ |
Tnfsf11 |
NNNTAAATGTTCGGACACAG |
NGC |
2 |
0.0129 |
Tier II |
| 7 |
NC_000070.6 |
4 |
84424648 |
+ |
Bnc2 |
NNNTTAATGTTCGGTCACAG |
NGT |
2 |
0.0059 |
Tier II |
| 8 |
NC_000076.6 |
10 |
31048072 |
+ |
Gm30676 |
NNNTAAATGTTCAGTCACAG |
NTG |
2 |
0.036 |
Tier III |
| 9 |
NC_000075.6 |
9 |
91477830 |
+ |
Gm29478 |
NNNTAAATGTTCGGTTACAG |
NCG |
2 |
0.033 |
Tier III |
Other clones with same target sequence:
(none)
