Construct: sgRNA BRDN0001148118

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GAAGATCTTAGATAAGCAGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PRKACB (5567)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75962

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000001.11 1 84182324 + PRKACB NNNGATCTTAGATAAGCAGA NGG 0 1.0 Tier I
2 NC_000011.10 11 78365207 + GAB2 NNNAATCTCAGATAAGCAGA NGG 2 0.8308 Tier II
3 NC_000011.10 11 89019013 - GRM5 NNNGATCTCAGATAAGCAAA NGG 2 0.6593 Tier II
4 NC_000011.10 11 9814290 - SBF2 NNNAATCTTAGAAAAGCAGA NGG 2 0.6231 Tier II
5 NC_000022.11 22 37481870 - MFNG NNNGATTTTAGGTAAGCAGA NGG 2 0.5868 Tier II
6 NC_000004.12 4 46383773 + GABRA2 NNNGAGTTTAGATAAGCAGA NGG 2 0.4643 Tier II
7 NC_000007.14 7 23266727 - GPNMB NNNCATCTTAGACAAGCAGA NGG 2 0.418 Tier II
8 NC_000001.11 1 217538961 - GPATCH2 NNNGCTTTTAGATAAGCAGA NGG 2 0.4063 Tier II
9 NC_000002.12 2 98912518 - CRACDL NNNGATCATAGATGAGCAGA NGG 2 0.3733 Tier II
10 NC_000001.11 1 178731965 + RALGPS2 NNNGATCTTATACAAGCAGA NGG 2 0.3036 Tier II
11 NC_000023.11 X 151735259 - CNGA2 NNNGATCTTAGATGAGCAGT NGG 2 0.28 Tier II
12 NC_000003.12 3 97325824 - EPHA6 NNNGATGTTAGATTAGCAGA NGG 2 0.251 Tier II
13 NC_000002.12 2 73239213 - CCT7 NNNAATCTTAGAGAAGCAGA NGG 2 0.2348 Tier II
14 NC_000009.12 9 33961568 + UBAP2 NNNGATCTTATATAAGTAGA NGG 2 0.1795 Tier II
15 NC_000005.10 5 59625631 + PDE4D NNNTATCTTAGATAAGAAGA NGG 2 0.1697 Tier II
16 NC_000019.10 19 39908830 - FCGBP NNNGGTCTTAGATATGCAGA NGG 2 0.144 Tier II
17 NC_000004.12 4 108993229 - COL25A1 NNNGATCTTACAGAAGCAGA NGG 2 0.1118 Tier II
18 NC_000011.10 11 117673294 - DSCAML1 NNNGATCTTAGAAAAGCAGA NGA 2 0.0481 Tier II
19 NC_000018.10 18 76876518 + ZNF236 NNNGATCTTAGATAAACAGA NTG 2 0.039 Tier II
20 NC_000009.12 9 21987280 + CDKN2A NNNGATCTTAGATAAGCATA NGT 2 0.0108 Tier II
21 NC_000020.11 20 43114153 - PTPRT NNNGATCTTAGAGAAGCAGA NTG 2 0.0102 Tier II
22 NC_000010.11 10 7648576 + ITIH5 NNNCATCTTAGATAAGCAGA NGT 2 0.0085 Tier II
23 NC_000017.11 17 13567334 - HS3ST3A1 NNNGATCTTAGATAACCAGA NGC 2 0.0 Tier II
24 NC_000005.10 5 135158105 - PITX1-AS1 NNNGAACTGAGATAAGCAGA NGG 2 0.5365 Tier III
25 NC_000003.12 3 97325824 - LOC101929278 NNNGATGTTAGATTAGCAGA NGG 2 0.251 Tier III
26 NC_000008.11 8 48102826 + LOC107986941 NNNGATCTTAGATAAGCAAA NAG 2 0.1852 Tier III
27 NC_000013.11 13 50212414 + DLEU1 NNNGATCTTAGAAAAGCAGA NAG 2 0.1795 Tier III
28 NC_000011.10 11 114845020 + LOC105369506 NNNGATGTTAGATAAGGAGA NGG 2 0.0277 Tier III
29 NC_000018.10 18 39243891 - MIR924HG NNNGATCTTAGAAAAGCAGA NTG 2 0.027 Tier III
30 NC_000009.12 9 21987280 + LOC124902130 NNNGATCTTAGATAAGCATA NGT 2 0.0108 Tier III
31 NC_000005.10 5 103667350 - LOC105379107 NNNGATCTTAGAGAATCAGA NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000069.6 3 146755637 - Prkacb NNNGATCTTAGACAAGCAGA NGG 1 0.7895 Tier I
2 NC_000074.6 8 83981296 + Prkaca NNNGATCTTAGACAAGCAGA NGG 1 0.7895 Tier I
3 NC_000067.6 1 52132587 + Stat1 NNNGATGTTAGATAAACAGA NGG 2 0.4706 Tier I
4 NC_000070.6 4 116223397 + Pik3r3 NNNGATTTTAAATAAGCAGA NGG 2 0.8125 Tier II
5 NC_000079.6 13 99508714 + Map1b NNNGATCTTTGATAAGCAGG NGG 2 0.6747 Tier II
6 NC_000068.7 2 14389855 - Slc39a12 NNNGATCTCAGAAAAGCAGA NGG 2 0.6391 Tier II
7 NC_000068.7 2 27063701 - Mymk NNNGATCTTAGATAGGCAGG NGG 2 0.4971 Tier II
8 NC_000080.6 14 118814083 - Cldn10 NNNGATCTGAGACAAGCAGA NGG 2 0.4887 Tier II
9 NC_000081.6 15 76811387 + Arhgap39 NNNGATCTGAGATAAGCATA NGG 2 0.4127 Tier II
10 NC_000067.6 1 123583737 + Dpp10 NNNGATATTAGATTAGCAGA NGG 2 0.4 Tier II
11 NC_000075.6 9 104180911 + Dnajc13 NNNGATCTTAGGTAAGCACA NGG 2 0.3238 Tier II
12 NC_000071.6 5 140205096 + Mad1l1 NNNGATCATATATAAGCAGA NGG 2 0.3077 Tier II
13 NC_000077.6 11 35895240 - Wwc1 NNNGAGCTTAGATAAGCTGA NGG 2 0.2857 Tier II
14 NC_000068.7 2 55538570 + Kcnj3 NNNGATCTTAGATTAGTAGA NGG 2 0.2489 Tier II
15 NC_000067.6 1 46463068 - Dnah7c NNNGATCTAAGCTAAGCAGA NGG 2 0.2444 Tier II
16 NC_000071.6 5 92673309 - Ccdc158 NNNGATCATAGAGAAGCAGA NGG 2 0.2087 Tier II
17 NC_000085.6 19 5506422 + Snx32 NNNGATCTTACATGAGCAGA NGG 2 0.2 Tier II
18 NC_000082.6 16 38174741 - Gsk3b NNNCTTCTTAGATAAGCAGA NGG 2 0.1925 Tier II
19 NC_000068.7 2 14963594 - Cacnb2 NNNGATCTTAGATAGGCAGA NAG 2 0.1685 Tier II
20 NC_000080.6 14 79562879 - Elf1 NNNCATCTTAGATAAGCAGA NAG 2 0.1373 Tier II
21 NC_000074.6 8 123364027 + Spire2 NNNGATCTTAGAAAAGGAGA NGG 2 0.0407 Tier II
22 NC_000071.6 5 19781189 + Magi2 NNNAATCTTAGATAAGCAGA NTG 2 0.0351 Tier II
23 NC_000082.6 16 39485826 + Gm36903 NNNGGTTTTAGATAAGCAGA NGG 2 0.585 Tier III
24 NC_000069.6 3 27156223 + 1700125G22Rik NNNGCTCTTTGATAAGCAGA NGG 2 0.4412 Tier III
25 NC_000086.7 X 161486280 - Prkaca-ps1 NNNGATCTTGGACAAGCAGA NGG 2 0.4386 Tier III
26 NC_000077.6 11 27495057 + Gm12077 NNNGATCTGAGATAAGGAGA NGG 2 0.0364 Tier III
27 NC_000071.6 5 19781189 + Gm52765 NNNAATCTTAGATAAGCAGA NTG 2 0.0351 Tier III
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Other clones with same target sequence:

(none)