Construct: sgRNA BRDN0001148120
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ATCCAACAGCCAGCTATACT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- AK3 (50808)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76584
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000009.12 |
9 |
4722524 |
+ |
AK3 |
NNNCAACAGCCAGCTATACT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000004.12 |
4 |
7864275 |
- |
AFAP1 |
NNNAAACAGCCAGCTATACT |
NGG |
1 |
0.8421 |
Tier II |
| 3 |
NC_000002.12 |
2 |
54577140 |
+ |
SPTBN1 |
NNNCAACAGCCAGCTAATCT |
NGG |
2 |
0.2667 |
Tier II |
| 4 |
NC_000010.11 |
10 |
19296140 |
+ |
MALRD1 |
NNNAAACAGCCATCTATACT |
NGG |
2 |
0.2526 |
Tier II |
| 5 |
NC_000012.12 |
12 |
122845049 |
- |
HIP1R |
NNNCAACTGCCAGCAATACT |
NGG |
2 |
0.2481 |
Tier II |
| 6 |
NC_000007.14 |
7 |
3334235 |
- |
SDK1 |
NNNGAAGAGCCAGCTATACT |
NGG |
2 |
0.2353 |
Tier II |
| 7 |
NC_000004.12 |
4 |
113132807 |
+ |
ANK2 |
NNNCAGCAGCCATCTATACT |
NGG |
2 |
0.2143 |
Tier II |
| 8 |
NC_000003.12 |
3 |
50392275 |
- |
CACNA2D2 |
NNNCACCAGCCAGCTATGCT |
NGG |
2 |
0.1818 |
Tier II |
| 9 |
NC_000011.10 |
11 |
79080680 |
- |
TENM4 |
NNNTAACAGCCAGCTGTACT |
NGG |
2 |
0.1538 |
Tier II |
| 10 |
NC_000001.11 |
1 |
17268396 |
- |
PADI3 |
NNNCAACAGCCATCTATATT |
NGG |
2 |
0.1385 |
Tier II |
| 11 |
NC_000003.12 |
3 |
129463872 |
+ |
IFT122 |
NNNCAACATCCAGCTATACT |
NAG |
2 |
0.1383 |
Tier II |
| 12 |
NC_000001.11 |
1 |
85888233 |
+ |
COL24A1 |
NNNCAACAGCCAGCCATTCT |
NGG |
2 |
0.1364 |
Tier II |
| 13 |
NC_000013.11 |
13 |
38829277 |
- |
FREM2 |
NNNCAACAGCCAGCTATCAT |
NGG |
2 |
0.0816 |
Tier II |
| 14 |
NC_000010.11 |
10 |
32944637 |
- |
ITGB1 |
NNNCAACAGCTAGCTATACT |
NAG |
2 |
0.0798 |
Tier II |
| 15 |
NC_000003.12 |
3 |
50185824 |
- |
SEMA3F |
NNNCAACAGCCAGCTAGGCT |
NGG |
2 |
0.0471 |
Tier II |
| 16 |
NC_000023.11 |
X |
56242659 |
- |
KLF8 |
NNNCAACAGCCAGCTAAACT |
NGA |
2 |
0.037 |
Tier II |
| 17 |
NC_000001.11 |
1 |
228172582 |
- |
IBA57 |
NNNCAGCAGCCAGCTATACT |
NGT |
2 |
0.0115 |
Tier II |
| 18 |
NC_000003.12 |
3 |
3770804 |
+ |
LOC100130207 |
NNNCCACAGACAGCTATACT |
NGG |
2 |
0.4333 |
Tier III |
| 19 |
NC_000007.14 |
7 |
3334235 |
- |
SDK1-AS1 |
NNNGAAGAGCCAGCTATACT |
NGG |
2 |
0.2353 |
Tier III |
| 20 |
NC_000008.11 |
8 |
64194841 |
- |
LINC01414 |
NNNCAACAGCCTGCTATGCT |
NGG |
2 |
0.1333 |
Tier III |
| 21 |
NC_000011.10 |
11 |
60834241 |
+ |
LOC105369322 |
NNNCAACAGCCTGCCATACT |
NGG |
2 |
0.0909 |
Tier III |
| 22 |
NC_000010.11 |
10 |
32944637 |
- |
AK3P5 |
NNNCAACAGCTAGCTATACT |
NAG |
2 |
0.0798 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000085.6 |
19 |
29037890 |
+ |
Ak3 |
NNNCAACAGCCAGCTACACT |
NGG |
1 |
0.7059 |
Tier I |
| 2 |
NC_000068.7 |
2 |
54484461 |
+ |
Galnt13 |
NNNCAAAAGACAGCTATACT |
NGG |
2 |
0.65 |
Tier II |
| 3 |
NC_000071.6 |
5 |
14109611 |
+ |
Sema3e |
NNNCAAGAGACAGCTATACT |
NGG |
2 |
0.4078 |
Tier II |
| 4 |
NC_000084.6 |
18 |
71527795 |
- |
Dcc |
NNNCAACAGCCAGTTAAACT |
NGG |
2 |
0.3911 |
Tier II |
| 5 |
NC_000078.6 |
12 |
79544275 |
- |
Rad51b |
NNNCCTCAGCCAGCTATACT |
NGG |
2 |
0.3571 |
Tier II |
| 6 |
NC_000076.6 |
10 |
5311850 |
+ |
Syne1 |
NNNCAGCAGCCAGCTATGCT |
NGG |
2 |
0.2857 |
Tier II |
| 7 |
NC_000071.6 |
5 |
100396323 |
- |
Sec31a |
NNNCCACAGCCAGCTATACA |
NGG |
2 |
0.2813 |
Tier II |
| 8 |
NC_000079.6 |
13 |
53151528 |
- |
Ror2 |
NNNCAACCGCCAGCTAAACT |
NGG |
2 |
0.2286 |
Tier II |
| 9 |
NC_000077.6 |
11 |
47775702 |
+ |
Sgcd |
NNNCCACAGCCAGCTATAAT |
NGG |
2 |
0.2143 |
Tier II |
| 10 |
NC_000068.7 |
2 |
33775703 |
+ |
Mvb12b |
NNNCAACGGCCAGCTATCCT |
NGG |
2 |
0.1397 |
Tier II |
| 11 |
NC_000070.6 |
4 |
45811638 |
- |
Igfbpl1 |
NNNCACCAGCCAGCCATACT |
NGG |
2 |
0.124 |
Tier II |
| 12 |
NC_000068.7 |
2 |
91635518 |
- |
F2 |
NNNCAGCAGCCAGCTATACT |
NCG |
2 |
0.0765 |
Tier II |
| 13 |
NC_000080.6 |
14 |
32634058 |
- |
Prrxl1 |
NNNCAACAGCCAGCTATACT |
NGA |
1 |
0.0694 |
Tier II |
| 14 |
NC_000076.6 |
10 |
69828556 |
- |
Ank3 |
NNNCAAGAGCCAGCTATACC |
NGG |
2 |
0.0428 |
Tier II |
| 15 |
NC_000067.6 |
1 |
87539079 |
- |
Ngef |
NNNGAACAGCCAGCTATACT |
NTG |
2 |
0.0195 |
Tier II |
| 16 |
NC_000068.7 |
2 |
91224871 |
- |
Ddb2 |
NNNAAACAGCCAGCTATACT |
NGT |
2 |
0.0136 |
Tier II |
| 17 |
NC_000075.6 |
9 |
108415481 |
+ |
Iho1 |
NNNCAACAGCCAGCTAAACT |
NGC |
2 |
0.0119 |
Tier II |
| 18 |
NC_000077.6 |
11 |
113426685 |
+ |
Slc39a11 |
NNNCAACAGCCAGCTATCCT |
NGT |
2 |
0.0031 |
Tier II |
| 19 |
NC_000082.6 |
16 |
60592907 |
+ |
Epha6 |
NNNCAACAGCCAGCTATACG |
NGT |
2 |
0.0028 |
Tier II |
| 20 |
NC_000073.6 |
7 |
57356976 |
- |
Gabrg3 |
NNNCAACAGCCAGCTTCACT |
NGG |
2 |
0.0 |
Tier II |
| 21 |
NC_000078.6 |
12 |
57406254 |
- |
Mipol1 |
NNNCAACAGCCAGCTTTACT |
NAG |
2 |
0.0 |
Tier II |
| 22 |
NC_000083.6 |
17 |
58113820 |
- |
Cntnap5c |
NNNCAACAGCCAGGTAGACT |
NGG |
2 |
0.0 |
Tier II |
| 23 |
NC_000074.6 |
8 |
66284804 |
- |
Marchf1 |
NNNCAACAGCCAGGTATGCT |
NGG |
2 |
0.0 |
Tier II |
| 24 |
NC_000073.6 |
7 |
91752749 |
- |
Dlg2 |
NNNCAACAGCCAGTTCTACT |
NGG |
2 |
0.0 |
Tier II |
| 25 |
NC_000071.6 |
5 |
35825095 |
- |
Ablim2 |
NNNCACCAGCCAGCTTTACT |
NGG |
2 |
0.0 |
Tier II |
| 26 |
NC_000070.6 |
4 |
84446961 |
+ |
Bnc2 |
NNNCAGCAGCCAGCTTTACT |
NGG |
2 |
0.0 |
Tier II |
| 27 |
NC_000078.6 |
12 |
30246752 |
- |
Sntg2 |
NNNCCACAGCCAGCTCTACT |
NGG |
2 |
0.0 |
Tier II |
Other clones with same target sequence:
(none)
