Construct: sgRNA BRDN0001148122
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AAACAATCACTGTTACTCCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CDK15 (65061)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76484
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
201807593 |
- |
CDK15 |
NNNCAATCACTGTTACTCCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000009.12 |
9 |
100101716 |
- |
INVS |
NNNCAATCGCTGTTACTCCT |
NGG |
2 |
0.4667 |
Tier II |
| 3 |
NC_000006.12 |
6 |
64918484 |
- |
EYS |
NNNCAATCAATGTTACTCCC |
NGG |
2 |
0.3714 |
Tier II |
| 4 |
NC_000008.11 |
8 |
138489068 |
+ |
FAM135B |
NNNCAATCACTGCTACTCCC |
NGG |
2 |
0.3383 |
Tier II |
| 5 |
NC_000004.12 |
4 |
168841589 |
- |
PALLD |
NNNCAATGACTGTCACTCCG |
NGG |
2 |
0.1837 |
Tier II |
| 6 |
NC_000001.11 |
1 |
121291438 |
- |
SRGAP2C |
NNNCAATCACTTTTACTCCG |
NAG |
2 |
0.0997 |
Tier II |
| 7 |
NC_000001.11 |
1 |
144988687 |
+ |
SRGAP2B |
NNNCAATCACTTTTACTCCG |
NAG |
2 |
0.0997 |
Tier II |
| 8 |
NC_000001.11 |
1 |
206309795 |
- |
SRGAP2 |
NNNCAATCACTTTTACTCCG |
NAG |
2 |
0.0997 |
Tier II |
| 9 |
NC_000005.10 |
5 |
125924192 |
+ |
LOC124901056 |
NNNCAATCACTGAGACTCCG |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000067.6 |
1 |
59257906 |
- |
Cdk15 |
NNNGAATCACTGTTACTCCG |
NGG |
1 |
0.5 |
Tier I |
| 2 |
NC_000073.6 |
7 |
25148474 |
+ |
Pou2f2 |
NNNCAATCCCAGTTACTCCG |
NGG |
2 |
0.4286 |
Tier II |
| 3 |
NC_000076.6 |
10 |
8460138 |
+ |
Ust |
NNNCAAGCACTGTTACTCCC |
NGG |
2 |
0.2521 |
Tier II |
| 4 |
NC_000086.7 |
X |
136614734 |
- |
Tceal3 |
NNNCAAGCACTGTTACTCCG |
NGA |
2 |
0.0408 |
Tier II |
| 5 |
NC_000076.6 |
10 |
107017032 |
+ |
Acss3 |
NNNCACTCACTGTGACTCCG |
NGG |
2 |
0.0 |
Tier II |
| 6 |
NC_000083.6 |
17 |
13308018 |
+ |
Gm36684 |
NNNCAATGACTGTTACTCAG |
NGG |
2 |
0.2755 |
Tier III |
Other clones with same target sequence:
(none)
