Construct: sgRNA BRDN0001148139
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGTGCGTTCCTAGCAACAGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- STK39 (27347)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76073
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
168140731 |
- |
STK39 |
NNNGCGTTCCTAGCAACAGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000003.12 |
3 |
147406375 |
- |
ZIC4 |
NNNGCGATCCTGGCAACAGG |
NGG |
2 |
0.6319 |
Tier II |
| 3 |
NC_000004.12 |
4 |
74773274 |
+ |
BTC |
NNNGCTTTCCTAGCAACAGC |
NGG |
2 |
0.2857 |
Tier II |
| 4 |
NC_000007.14 |
7 |
133822119 |
- |
EXOC4 |
NNNGCTTTCCTTGCAACAGG |
NGG |
2 |
0.2222 |
Tier II |
| 5 |
NC_000008.11 |
8 |
84921690 |
- |
RALYL |
NNNGAGTTCCTAGGAACAGG |
NGG |
2 |
0.0 |
Tier II |
| 6 |
NC_000007.14 |
7 |
133822119 |
- |
LOC101928861 |
NNNGCTTTCCTTGCAACAGG |
NGG |
2 |
0.2222 |
Tier III |
| 7 |
NC_000013.11 |
13 |
84283570 |
- |
LINC00333 |
NNNGCGTTCCTAGCAGCAGG |
NGC |
2 |
0.0043 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
98572561 |
- |
Cfap299 |
NNNGCATTCCTAGCAACAGG |
NGG |
1 |
1.0 |
Tier II |
| 2 |
NC_000073.6 |
7 |
127231747 |
- |
Septin1 |
NNNGCGTTCATAACAACAGG |
NGG |
2 |
0.8 |
Tier II |
| 3 |
NC_000074.6 |
8 |
72179322 |
+ |
Rab8a |
NNNGTGTTGCTAGCAACAGG |
NGG |
2 |
0.3939 |
Tier II |
| 4 |
NC_000079.6 |
13 |
52908333 |
- |
Auh |
NNNGCTTTCCTAGCAACAGC |
NGG |
2 |
0.2857 |
Tier II |
| 5 |
NC_000080.6 |
14 |
57372425 |
- |
Cryl1 |
NNNGCGTGCCTAGCATCAGG |
NGG |
2 |
0.0 |
Tier II |
| 6 |
NC_000083.6 |
17 |
23601175 |
+ |
Zscan10 |
NNNGCGTTCCTAGCACCAGA |
NGG |
2 |
0.0 |
Tier II |
| 7 |
NC_000071.6 |
5 |
98572561 |
- |
Gm42122 |
NNNGCATTCCTAGCAACAGG |
NGG |
1 |
1.0 |
Tier III |
| 8 |
NC_000071.6 |
5 |
93391934 |
+ |
Kat2b-ps |
NNNGCTTTCCTAGTAACAGG |
NGG |
2 |
0.4889 |
Tier III |
Other clones with same target sequence:
(none)
