Construct: sgRNA BRDN0001148140
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ACAACAACATGGATTGACGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- DAPK1 (1612)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76519
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000009.12 |
9 |
87697093 |
- |
DAPK1 |
NNNACAACATGGATTGACGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000009.12 |
9 |
29025941 |
- |
LINGO2 |
NNNACAACATGGATTAACCT |
NGG |
2 |
0.4483 |
Tier II |
| 3 |
NC_000007.14 |
7 |
117136648 |
+ |
ST7 |
NNNACAACATGGATTGAAAT |
NGG |
2 |
0.3846 |
Tier II |
| 4 |
NC_000016.10 |
16 |
88147361 |
- |
ZNF469 |
NNNACAACATGGACTGACTT |
NGG |
2 |
0.1905 |
Tier II |
| 5 |
NC_000006.12 |
6 |
136856728 |
+ |
PEX7 |
NNNACAACATGGATTGAGGA |
NGG |
2 |
0.075 |
Tier II |
| 6 |
NC_000012.12 |
12 |
105057613 |
+ |
ALDH1L2 |
NNNACAACATGGATGAACGT |
NGG |
2 |
0.05 |
Tier II |
| 7 |
NC_000012.12 |
12 |
4515506 |
+ |
C12orf4 |
NNNACAACATGGATTGACCT |
NGA |
2 |
0.0311 |
Tier II |
| 8 |
NC_000013.11 |
13 |
98903846 |
+ |
DOCK9 |
NNNACAACATGGATTGACCT |
NGA |
2 |
0.0311 |
Tier II |
| 9 |
NC_000007.14 |
7 |
66346130 |
- |
TPST1 |
NNNACAACATGGATGGAAGT |
NGG |
2 |
0.0269 |
Tier II |
| 10 |
NC_000002.12 |
2 |
71501706 |
- |
DYSF |
NNNACAACATGGATGGACCT |
NGG |
2 |
0.0224 |
Tier II |
| 11 |
NC_000003.12 |
3 |
41368689 |
- |
ULK4 |
NNNACAACATGGATGGACCT |
NGG |
2 |
0.0224 |
Tier II |
| 12 |
NC_000005.10 |
5 |
64951791 |
- |
CWC27 |
NNNACAACATGGATGGACCT |
NGG |
2 |
0.0224 |
Tier II |
| 13 |
NC_000003.12 |
3 |
41475202 |
+ |
ULK4 |
NNNACAACATGGATGGACGT |
NGA |
2 |
0.0035 |
Tier II |
| 14 |
NC_000011.10 |
11 |
114694081 |
+ |
NXPE2 |
NNNACAACATGGAGTGACAT |
NGG |
2 |
0.0 |
Tier II |
| 15 |
NC_000007.14 |
7 |
79824283 |
- |
LOC105375371 |
NNNACAACATGGATTAACTT |
NGG |
2 |
0.6667 |
Tier III |
| 16 |
NC_000007.14 |
7 |
117136648 |
+ |
ST7-AS2 |
NNNACAACATGGATTGAAAT |
NGG |
2 |
0.3846 |
Tier III |
| 17 |
NC_000007.14 |
7 |
118523912 |
- |
LOC105375473 |
NNNACAACATGGATTAACGT |
NGA |
2 |
0.0694 |
Tier III |
| 18 |
NC_000023.11 |
X |
33804766 |
- |
LOC105373153 |
NNNACAACATGGATTGAAGT |
NGA |
2 |
0.0374 |
Tier III |
| 19 |
NC_000002.12 |
2 |
103940357 |
+ |
LINC01965 |
NNNACAACATGGATGGACCT |
NGG |
2 |
0.0224 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000083.6 |
17 |
18278182 |
+ |
Vmn2r94 |
NNNACAATATGGATTGATGT |
NGG |
2 |
0.5625 |
Tier II |
| 2 |
NC_000080.6 |
14 |
124302838 |
+ |
Fgf14 |
NNNTCAACATGGATTGACCT |
NGG |
2 |
0.2853 |
Tier II |
| 3 |
NC_000071.6 |
5 |
23223514 |
+ |
Lhfpl3 |
NNNACACCATGGATTGATGT |
NGG |
2 |
0.2812 |
Tier II |
| 4 |
NC_000082.6 |
16 |
4002645 |
- |
Slx4 |
NNNACAACATGGAGTGAGGT |
NGG |
2 |
0.0 |
Tier II |
| 5 |
NC_000072.6 |
6 |
15638254 |
+ |
Gm36352 |
NNNACAACATGGTTTGACGT |
NGT |
2 |
0.0048 |
Tier III |
Other clones with same target sequence:
(none)
