Construct: sgRNA BRDN0001148141
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GAAGACGCCTACATTCTGCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TRIB2 (28951)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75603
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
12740368 |
+ |
TRIB2 |
NNNGACGCCTACATTCTGCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000017.11 |
17 |
20243808 |
- |
SPECC1 |
NNNGACGCCTACATTCTGCT |
NAG |
2 |
0.1815 |
Tier II |
| 3 |
NC_000006.12 |
6 |
29976629 |
- |
HCG9 |
NNNGGCGCCTACAGTCTGCG |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000078.6 |
12 |
15794035 |
- |
Trib2 |
NNNGACGCTTACATTCTCCG |
NGG |
2 |
0.4167 |
Tier I |
| 2 |
NC_000077.6 |
11 |
62211678 |
- |
Specc1 |
NNNGATGCCTACACTCTGCG |
NGG |
2 |
0.2653 |
Tier I |
| 3 |
NC_000071.6 |
5 |
118096583 |
+ |
Fbxw8 |
NNNGTCGCCTACATTCTGCC |
NGG |
2 |
0.1558 |
Tier II |
| 4 |
NC_000078.6 |
12 |
27187776 |
+ |
Gm40872 |
NNNGACGTCTACATTCTGCA |
NGG |
2 |
0.8203 |
Tier III |
| 5 |
NC_000071.6 |
5 |
98849195 |
+ |
Gm34563 |
NNNGACCCCTCCATTCTGCG |
NGG |
2 |
0.275 |
Tier III |
| 6 |
NC_000085.6 |
19 |
27157842 |
- |
Gm35438 |
NNNGACGCCTACATTTTGCG |
NGT |
2 |
0.005 |
Tier III |
Other clones with same target sequence:
(none)
