Construct: sgRNA BRDN0001148141

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GAAGACGCCTACATTCTGCG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TRIB2 (28951)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75603

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000002.12 2 12740368 + TRIB2 NNNGACGCCTACATTCTGCG NGG 0 1.0 Tier I
2 NC_000017.11 17 20243808 - SPECC1 NNNGACGCCTACATTCTGCT NAG 2 0.1815 Tier II
3 NC_000006.12 6 29976629 - HCG9 NNNGGCGCCTACAGTCTGCG NGG 2 0.0 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000078.6 12 15794035 - Trib2 NNNGACGCTTACATTCTCCG NGG 2 0.4167 Tier I
2 NC_000077.6 11 62211678 - Specc1 NNNGATGCCTACACTCTGCG NGG 2 0.2653 Tier I
3 NC_000071.6 5 118096583 + Fbxw8 NNNGTCGCCTACATTCTGCC NGG 2 0.1558 Tier II
4 NC_000078.6 12 27187776 + Gm40872 NNNGACGTCTACATTCTGCA NGG 2 0.8203 Tier III
5 NC_000071.6 5 98849195 + Gm34563 NNNGACCCCTCCATTCTGCG NGG 2 0.275 Tier III
6 NC_000085.6 19 27157842 - Gm35438 NNNGACGCCTACATTTTGCG NGT 2 0.005 Tier III
Download CSV

Other clones with same target sequence:

(none)