Construct: sgRNA BRDN0001148142

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AAACAGTGGCAAAACAACAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NMRK1 (54981)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75831

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000009.12 9 75077558 - NMRK1 NNNCAGTGGCAAAACAACAC NGG 0 1.0 Tier I
2 NC_000011.10 11 7602650 + PPFIBP2 NNNCAATGTCAAAACAACAC NGG 2 0.5333 Tier II
3 NC_000003.12 3 66134134 + SLC25A26 NNNCAGTGGAAAAACAAAAC NGG 2 0.4667 Tier II
4 NC_000018.10 18 58313836 + NEDD4L NNNCCCTGGCAAAACAACAC NGG 2 0.3409 Tier II
5 NC_000006.12 6 65631498 - EYS NNNCAGAGGGAAAACAACAC NGG 2 0.3403 Tier II
6 NC_000005.10 5 9068680 - SEMA5A NNNTAGTGGCAAAACAACAT NGG 2 0.24 Tier II
7 NC_000005.10 5 21788378 + CDH12 NNNCAGTAGCAAAACGACAC NGG 2 0.1923 Tier II
8 NC_000015.10 15 99574869 + MEF2A NNNCAGTGGCAAAACATCAC NGG 1 0.1333 Tier II
9 NC_000022.11 22 29631385 - NF2 NNNCAGTGGCAACACAACTC NGG 2 0.1134 Tier II
10 NC_000003.12 3 197125817 + DLG1 NNNCAGTGGCAAAACGACAA NGG 2 0.0962 Tier II
11 NC_000018.10 18 45663766 - SLC14A2 NNNCAGGGGCAAAACAAGAC NGG 2 0.0784 Tier II
12 NC_000017.11 17 66720683 - PRKCA NNNCACTGGCAAAACAACAC NGA 2 0.0473 Tier II
13 NC_000011.10 11 83681230 + DLG2 NNNCAGAGGCAAAACAACAC NGC 2 0.0194 Tier II
14 NC_000018.10 18 50184278 + MYO5B NNNCAGTGGCAAAAGAACAC NAG 2 0.013 Tier II
15 NC_000008.11 8 61165255 + CLVS1 NNNCAGTGGCAAAACAGCAG NGG 2 0.0104 Tier II
16 NC_000004.12 4 82629600 + SCD5 NNNCAGTGGCAAAATAACAC NGT 2 0.0011 Tier II
17 NC_000011.10 11 118754971 + DDX6 NNNCAGTGGCAAAACCACAC NGG 1 0.0 Tier II
18 NC_000001.11 1 198196526 + NEK7 NNNCAGTGGCAAAACTACAA NGG 2 0.0 Tier II
19 NC_000005.10 5 115160556 + TRIM36 NNNCAGTGGCAAAACTAGAC NGG 2 0.0 Tier II
20 NC_000020.11 20 22940117 - LOC107985449 NNNCAGAGGCAATACAACAC NGG 2 0.2625 Tier III
21 NC_000018.10 18 45663766 - LOC105372093 NNNCAGGGGCAAAACAAGAC NGG 2 0.0784 Tier III
22 NC_000001.11 1 193960747 + LOC124904475 NNNAAGTGGCAAAAGAACAC NGG 2 0.0421 Tier III
23 NC_000014.9 14 74841825 + LOC105370569 NNNCAGTGGCAAAACAAGAC NAG 2 0.0346 Tier III
24 NC_000004.12 4 186525013 - LOC105377596 NNNCAGTGGCAAAGGAACAC NGG 2 0.0233 Tier III
25 NC_000011.10 11 83681230 + DLG2-AS2 NNNCAGAGGCAAAACAACAC NGC 2 0.0194 Tier III
26 NC_000003.12 3 106487217 + LOC101929485 NNNCAGTGGCAAAACAGCAG NGG 2 0.0104 Tier III
27 NC_000012.12 12 22595377 - ETNK1-DT NNNCAGTGGCAAAACAACAA NGT 2 0.0081 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000072.6 6 88446416 - Eefsec NNNCAGTGGCAAAACAGCGC NGG 2 0.0662 Tier I
2 NC_000080.6 14 96431988 + Klhl1 NNNCGTTGGCAAAACAACAC NGG 2 0.48 Tier II
3 NC_000075.6 9 44143151 + Cbl NNNCACTGGCAAGACAACAC NGG 2 0.4447 Tier II
4 NC_000075.6 9 44724120 - Phldb1 NNNCAGTGGTAAAGCAACAC NGG 2 0.4392 Tier II
5 NC_000079.6 13 45896484 + Atxn1 NNNGAGTGGAAAAACAACAC NGG 2 0.4333 Tier II
6 NC_000070.6 4 49715088 - Grin3a NNNCAGTTTCAAAACAACAC NGG 2 0.3333 Tier II
7 NC_000070.6 4 154628650 + Prdm16 NNNCAGTGGCCAAACAATAC NGG 2 0.2571 Tier II
8 NC_000081.6 15 41858649 - Oxr1 NNNCAGTGAGAAAACAACAC NGG 2 0.25 Tier II
9 NC_000074.6 8 111078466 - Clec18a NNNTAGTGGCAATACAACAC NGG 2 0.24 Tier II
10 NC_000067.6 1 126600984 + Nckap5 NNNCAGAGGCAAAACAACCC NGG 2 0.181 Tier II
11 NC_000074.6 8 117200620 - Gan NNNCACTGGCAACACAACAC NGG 2 0.1435 Tier II
12 NC_000078.6 12 55109245 + Srp54a NNNCAGTGTCAAAACAACAC NAG 2 0.1383 Tier II
13 NC_000078.6 12 55184305 + Srp54b NNNCAGTGTCAAAACAACAC NAG 2 0.1383 Tier II
14 NC_000078.6 12 55258170 + Srp54c NNNCAGTGTCAAAACAACAC NAG 2 0.1383 Tier II
15 NC_000072.6 6 77352583 + Ctnna2 NNNCAGTGGCAAGACGACAC NGG 2 0.1254 Tier II
16 NC_000076.6 10 61449141 + Eif4ebp2 NNNCACTGGCAAAACAGCAC NGG 2 0.1203 Tier II
17 NC_000080.6 14 75501876 + Siah3 NNNCAGAGGCAAAACAAGAC NGG 2 0.1167 Tier II
18 NC_000079.6 13 100775443 + Cenph NNNCAGTGGCAAGACAGCAC NGG 2 0.1151 Tier II
19 NC_000080.6 14 23332280 - Kcnma1 NNNCAGTGACAAAACAAGAC NGG 2 0.0857 Tier II
20 NC_000067.6 1 180121229 - Cdc42bpa NNNCAGTGGCATCACAACAC NGG 2 0.0702 Tier II
21 NC_000067.6 1 180121327 - Cdc42bpa NNNCAGTGGCATCACAACAC NGG 2 0.0702 Tier II
22 NC_000067.6 1 180121548 - Cdc42bpa NNNCAGTGGCATCACAACAC NGG 2 0.0702 Tier II
23 NC_000077.6 11 55135675 + Slc36a3 NNNCAGTGGCTAAAAAACAC NGG 2 0.0684 Tier II
24 NC_000083.6 17 5234136 + Arid1b NNNCAGTGGCTAAAAAACAC NGG 2 0.0684 Tier II
25 NC_000083.6 17 14825403 + Wdr27 NNNCAGTGGAAAAACAACAC NGA 2 0.0602 Tier II
26 NC_000085.6 19 50360879 + Sorcs1 NNNCAGTGGTAAAACAACAC NGC 2 0.0209 Tier II
27 NC_000076.6 10 110101240 - Nav3 NNNCTGTGGCAAAACAACAC NTG 2 0.0142 Tier II
28 NC_000071.6 5 22960856 - Lhfpl3 NNNCTGTGGCAAAACAACAC NGC 2 0.0081 Tier II
29 NC_000080.6 14 99974088 - Klf12 NNNCAGTGGCAAAACAACAG NTG 2 0.0023 Tier II
30 NC_000078.6 12 79065152 + Plekhh1 NNNCAGTAGCAAAACTACAC NGG 2 0.0 Tier II
31 NC_000067.6 1 190181885 + Prox1os NNNCAATGGAAAAACAACAC NGG 2 0.8667 Tier III
32 NC_000077.6 11 24503060 - 4933430M04Rik NNNCAGGGGCAAAACAACAA NGG 2 0.2941 Tier III
33 NC_000068.7 2 137674693 - Gm14064 NNNCTGTGGCAAAACAACAA NGG 2 0.1818 Tier III
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Other clones with same target sequence:

(none)