Construct: sgRNA BRDN0001148143
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CAGCGGCATCAAACTCAACG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MKNK2 (2872)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77764
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000019.10 |
19 |
2042471 |
- |
MKNK2 |
NNNCGGCATCAAACTCAACG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000011.10 |
11 |
4825865 |
- |
MMP26 |
NNNCAGCATCAAACTCAATG |
NGG |
2 |
0.4 |
Tier II |
| 3 |
NC_000013.11 |
13 |
28977008 |
- |
MTUS2 |
NNNTGGCATCAAACTCAAAG |
NGG |
2 |
0.3429 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
77306425 |
+ |
Noa1 |
NNNCGGCATCAAACTCAAAG |
NGA |
2 |
0.0298 |
Tier I |
| 2 |
NC_000084.6 |
18 |
63709911 |
+ |
Wdr7 |
NNNTGGCTTCAAACTCAACG |
NGG |
2 |
0.3429 |
Tier II |
| 3 |
NC_000081.6 |
15 |
91963069 |
- |
Gm18979 |
NNNGGACATCAAACTCAACG |
NGG |
2 |
0.5 |
Tier III |
| 4 |
NC_000072.6 |
6 |
48177643 |
- |
Gm16630 |
NNNGGGCCTCAAACTCAACG |
NGG |
2 |
0.2143 |
Tier III |
Other clones with same target sequence:
(none)
