Construct: sgRNA BRDN0001148145

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TACGGATCACCAAATCTTAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NO_SITE control
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
80210

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000001.11 1 222558728 - TAF1A NNNAGATCACCAAATCTTAG NGA 2 0.0625 Tier I
2 NC_000004.12 4 90877758 - CCSER1 NNNAGATCTCCAAATCTTAG NGG 2 0.54 Tier II
3 NC_000001.11 1 245393509 - KIF26B NNNGGATCACCAAATATTTG NGG 2 0.5385 Tier II
4 NC_000011.10 11 8129788 - RIC3 NNNAGATCACCAAATCTTTG NGG 2 0.4846 Tier II
5 NC_000006.12 6 161538493 + PRKN NNNGGACCTCCAAATCTTAG NGG 2 0.4125 Tier II
6 NC_000011.10 11 22627444 + GAS2 NNNGGGTCACCAAGTCTTAG NGG 2 0.3333 Tier II
7 NC_000005.10 5 11754129 - CTNND2 NNNGGATCAACAAATCTTGG NGG 2 0.325 Tier II
8 NC_000005.10 5 37662891 + WDR70 NNNGGATCATCAAATCTGAG NGG 2 0.3137 Tier II
9 NC_000013.11 13 35889651 - DCLK1 NNNGTATCACCAAATCTTAA NGG 2 0.2813 Tier II
10 NC_000003.12 3 48845988 + PRKAR2A NNNGGATCACCAAATCTGTG NGG 2 0.1795 Tier II
11 NC_000008.11 8 72929675 + KCNB2 NNNGAATCACCAAATGTTAG NGG 2 0.1333 Tier II
12 NC_000002.12 2 102196366 - IL1RL2 NNNGGATCACCAAATCTGAG NAG 2 0.0864 Tier II
13 NC_000010.11 10 66209246 - CTNNA3 NNNGTATCACCAAATCTTAG NAG 2 0.0778 Tier II
14 NC_000013.11 13 35889651 - LOC105370163 NNNGTATCACCAAATCTTAA NGG 2 0.2813 Tier III
15 NC_000012.12 12 102573527 - LINC02456 NNNGGTTCACCAAATCTTAG NGA 2 0.0496 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000079.6 13 75730902 - Ell2 NNNGGACCACCAAATCTAAG NGG 2 0.4583 Tier II
2 NC_000069.6 3 100827130 - Vtcn1 NNNGGGTCACTAAATCTTAG NGG 2 0.2198 Tier II
3 NC_000082.6 16 34159658 - Kalrn NNNGCATCACCAAATCTTAG NAG 2 0.2037 Tier II
4 NC_000069.6 3 79690041 + Rxfp1 NNNGGATGACCAAATCTTAG NAG 2 0.1667 Tier II
5 NC_000069.6 3 159846095 - Wls NNNGGATCACCAAATTTCAG NGG 2 0.1319 Tier II
6 NC_000084.6 18 38652548 - Arhgap26 NNNTGATCACCTAATCTTAG NGG 2 0.1212 Tier II
7 NC_000086.7 X 84879411 - Dmd NNNTGATCACCAAATCTTAG NGT 2 0.0059 Tier II
8 NC_000079.6 13 81038764 - 9330111N05Rik NNNGGATCTCCAAATCTTGG NGG 2 0.225 Tier III
Download CSV

Other clones with same target sequence:

(none)

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