Construct: sgRNA BRDN0001148145
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TACGGATCACCAAATCTTAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NO_SITE control
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 80210
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
222558728 |
- |
TAF1A |
NNNAGATCACCAAATCTTAG |
NGA |
2 |
0.0625 |
Tier I |
| 2 |
NC_000004.12 |
4 |
90877758 |
- |
CCSER1 |
NNNAGATCTCCAAATCTTAG |
NGG |
2 |
0.54 |
Tier II |
| 3 |
NC_000001.11 |
1 |
245393509 |
- |
KIF26B |
NNNGGATCACCAAATATTTG |
NGG |
2 |
0.5385 |
Tier II |
| 4 |
NC_000011.10 |
11 |
8129788 |
- |
RIC3 |
NNNAGATCACCAAATCTTTG |
NGG |
2 |
0.4846 |
Tier II |
| 5 |
NC_000006.12 |
6 |
161538493 |
+ |
PRKN |
NNNGGACCTCCAAATCTTAG |
NGG |
2 |
0.4125 |
Tier II |
| 6 |
NC_000011.10 |
11 |
22627444 |
+ |
GAS2 |
NNNGGGTCACCAAGTCTTAG |
NGG |
2 |
0.3333 |
Tier II |
| 7 |
NC_000005.10 |
5 |
11754129 |
- |
CTNND2 |
NNNGGATCAACAAATCTTGG |
NGG |
2 |
0.325 |
Tier II |
| 8 |
NC_000005.10 |
5 |
37662891 |
+ |
WDR70 |
NNNGGATCATCAAATCTGAG |
NGG |
2 |
0.3137 |
Tier II |
| 9 |
NC_000013.11 |
13 |
35889651 |
- |
DCLK1 |
NNNGTATCACCAAATCTTAA |
NGG |
2 |
0.2813 |
Tier II |
| 10 |
NC_000003.12 |
3 |
48845988 |
+ |
PRKAR2A |
NNNGGATCACCAAATCTGTG |
NGG |
2 |
0.1795 |
Tier II |
| 11 |
NC_000008.11 |
8 |
72929675 |
+ |
KCNB2 |
NNNGAATCACCAAATGTTAG |
NGG |
2 |
0.1333 |
Tier II |
| 12 |
NC_000002.12 |
2 |
102196366 |
- |
IL1RL2 |
NNNGGATCACCAAATCTGAG |
NAG |
2 |
0.0864 |
Tier II |
| 13 |
NC_000010.11 |
10 |
66209246 |
- |
CTNNA3 |
NNNGTATCACCAAATCTTAG |
NAG |
2 |
0.0778 |
Tier II |
| 14 |
NC_000013.11 |
13 |
35889651 |
- |
LOC105370163 |
NNNGTATCACCAAATCTTAA |
NGG |
2 |
0.2813 |
Tier III |
| 15 |
NC_000012.12 |
12 |
102573527 |
- |
LINC02456 |
NNNGGTTCACCAAATCTTAG |
NGA |
2 |
0.0496 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000079.6 |
13 |
75730902 |
- |
Ell2 |
NNNGGACCACCAAATCTAAG |
NGG |
2 |
0.4583 |
Tier II |
| 2 |
NC_000069.6 |
3 |
100827130 |
- |
Vtcn1 |
NNNGGGTCACTAAATCTTAG |
NGG |
2 |
0.2198 |
Tier II |
| 3 |
NC_000082.6 |
16 |
34159658 |
- |
Kalrn |
NNNGCATCACCAAATCTTAG |
NAG |
2 |
0.2037 |
Tier II |
| 4 |
NC_000069.6 |
3 |
79690041 |
+ |
Rxfp1 |
NNNGGATGACCAAATCTTAG |
NAG |
2 |
0.1667 |
Tier II |
| 5 |
NC_000069.6 |
3 |
159846095 |
- |
Wls |
NNNGGATCACCAAATTTCAG |
NGG |
2 |
0.1319 |
Tier II |
| 6 |
NC_000084.6 |
18 |
38652548 |
- |
Arhgap26 |
NNNTGATCACCTAATCTTAG |
NGG |
2 |
0.1212 |
Tier II |
| 7 |
NC_000086.7 |
X |
84879411 |
- |
Dmd |
NNNTGATCACCAAATCTTAG |
NGT |
2 |
0.0059 |
Tier II |
| 8 |
NC_000079.6 |
13 |
81038764 |
- |
9330111N05Rik |
NNNGGATCTCCAAATCTTGG |
NGG |
2 |
0.225 |
Tier III |
Other clones with same target sequence:
(none)
