Construct: sgRNA BRDN0001148146
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGTGAGCTTATATAACAACC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- GK5 (256356)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75774
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000003.12 |
3 |
142187725 |
+ |
GK5 |
NNNGAGCTTATATAACAACC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000009.12 |
9 |
131698902 |
- |
RAPGEF1 |
NNNGAGCTTAAATAACAACT |
NGG |
2 |
0.225 |
Tier II |
| 3 |
NC_000016.10 |
16 |
7339098 |
- |
RBFOX1 |
NNNGAGCCTATATATCAACC |
NGG |
2 |
0.2 |
Tier II |
| 4 |
NC_000012.12 |
12 |
7329835 |
+ |
CD163L1 |
NNNGAGCTTGTATAACAACC |
NAG |
2 |
0.144 |
Tier II |
| 5 |
NC_000005.10 |
5 |
60361666 |
+ |
PDE4D |
NNNGAGCTTATTTAACCACC |
NGG |
2 |
0.0588 |
Tier II |
| 6 |
NC_000007.14 |
7 |
127883191 |
- |
SND1 |
NNNGAGCTTATATTACAACC |
NGC |
2 |
0.0119 |
Tier II |
| 7 |
NC_000006.12 |
6 |
52890504 |
- |
GSTA10P |
NNNGAGGTTATAAAACAACC |
NGG |
2 |
0.3258 |
Tier III |
| 8 |
NC_000006.12 |
6 |
52890504 |
- |
LOC105375091 |
NNNGAGGTTATAAAACAACC |
NGG |
2 |
0.3258 |
Tier III |
| 9 |
NC_000001.11 |
1 |
171179933 |
- |
LOC105371611 |
NNNGAGCTTATGTAACAGCC |
NGG |
2 |
0.2889 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000075.6 |
9 |
96145727 |
- |
Gk5 |
NNNGAGCTTGTATAACAGCC |
NGG |
2 |
0.2222 |
Tier I |
| 2 |
NC_000080.6 |
14 |
28511933 |
- |
Wnt5a |
NNNTAGCTTCTATAACAACC |
NGG |
2 |
0.1212 |
Tier I |
| 3 |
NC_000067.6 |
1 |
85974808 |
+ |
Gpr55 |
NNNGAGCCTATATAAAAACC |
NGG |
2 |
1.0 |
Tier II |
| 4 |
NC_000083.6 |
17 |
67927920 |
+ |
Arhgap28 |
NNNGAGCTTATGTAACAAAC |
NGG |
2 |
0.3095 |
Tier II |
| 5 |
NC_000076.6 |
10 |
128565138 |
- |
Pa2g4 |
NNNGAGCTTCTAAAACAACC |
NGG |
2 |
0.2308 |
Tier II |
| 6 |
NC_000071.6 |
5 |
140930153 |
- |
Card11 |
NNNGAGCTTATATAAGAACC |
NGG |
1 |
0.1538 |
Tier II |
| 7 |
NC_000081.6 |
15 |
59509004 |
+ |
Nsmce2 |
NNNGAGCTTATATAACAACA |
NAG |
2 |
0.1296 |
Tier II |
| 8 |
NC_000072.6 |
6 |
52736083 |
+ |
Tax1bp1 |
NNNGAACTTATATAACAAGC |
NGG |
2 |
0.125 |
Tier II |
| 9 |
NC_000074.6 |
8 |
56116030 |
- |
Glra3 |
NNNGAGGTTATAGAACAACC |
NGG |
2 |
0.1228 |
Tier II |
| 10 |
NC_000074.6 |
8 |
38470051 |
- |
Sgcz |
NNNGGGCTTATATAACAAGC |
NGG |
2 |
0.09 |
Tier II |
| 11 |
NC_000086.7 |
X |
166299567 |
+ |
Gpm6b |
NNNGAGCTTATATAACAAAG |
NGG |
2 |
0.0252 |
Tier II |
| 12 |
NC_000068.7 |
2 |
19388719 |
+ |
Msrb2 |
NNNGAGCTTATTTAACAACC |
NGC |
2 |
0.0074 |
Tier II |
Other clones with same target sequence:
(none)
