Construct: sgRNA BRDN0001148151
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GCAGCGAAAAGACAGCGTGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NEK6 (10783)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76258
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000009.12 |
9 |
124312522 |
- |
NEK6 |
NNNGCGAAAAGACAGCGTGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000020.11 |
20 |
14322631 |
+ |
MACROD2 |
NNNGCCAAAAGACAGAGTGT |
NGG |
2 |
0.6818 |
Tier II |
| 3 |
NC_000007.14 |
7 |
92258791 |
+ |
ANKIB1 |
NNNGGGAAAAGACAGAGTGT |
NGG |
2 |
0.6 |
Tier II |
| 4 |
NC_000016.10 |
16 |
6539869 |
- |
RBFOX1 |
NNNGCAAAAAGACAGCTTGT |
NGG |
2 |
0.25 |
Tier II |
| 5 |
NC_000001.11 |
1 |
174925080 |
+ |
RABGAP1L |
NNNGAGAAAAGACAGCGGGT |
NGG |
2 |
0.1905 |
Tier II |
| 6 |
NC_000002.12 |
2 |
241297098 |
+ |
HDLBP |
NNNGCAAAAAGAGAGCGTGT |
NGG |
2 |
0.1364 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000084.6 |
18 |
74949184 |
+ |
Lipg |
NNNGCCAAAGGACAGCGTGT |
NGG |
2 |
0.3788 |
Tier I |
| 2 |
NC_000085.6 |
19 |
53922082 |
+ |
Pdcd4 |
NNNGCGGAAAGACAGTGTGT |
NGG |
2 |
0.2172 |
Tier I |
| 3 |
NC_000068.7 |
2 |
38557829 |
- |
Nek6 |
NNNGCGAAAAGACAGGGTGT |
NTG |
2 |
0.006 |
Tier I |
| 4 |
NC_000071.6 |
5 |
81098403 |
+ |
Adgrl3 |
NNNGGGAAAAGACAGCGAGT |
NGG |
2 |
0.4 |
Tier II |
| 5 |
NC_000069.6 |
3 |
63839075 |
- |
Plch1 |
NNNGAGAAAAGACAGGGTGT |
NGG |
2 |
0.0879 |
Tier II |
| 6 |
NC_000069.6 |
3 |
141691938 |
- |
Unc5c |
NNNGCGAAAAGGCAGCGTGT |
NTG |
2 |
0.0281 |
Tier II |
| 7 |
NC_000072.6 |
6 |
127155036 |
- |
9330179D12Rik |
NNNGCGAAAAAGCAGCGTGT |
NGG |
2 |
0.7222 |
Tier III |
Other clones with same target sequence:
(none)
