Construct: sgRNA BRDN0001148153
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TCCCGCAGGGCCACAAACGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TNK2 (10188)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75582
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000003.12 |
3 |
195878476 |
+ |
TNK2 |
NNNCGCAGGGCCACAAACGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
166989612 |
- |
MAEL |
NNNCCCAGGGCCACAAACGG |
NGG |
2 |
0.1387 |
Tier II |
| 3 |
NC_000008.11 |
8 |
12813143 |
+ |
LINC00681 |
NNNCGCAAGGCAACAAACGT |
NGG |
2 |
0.7143 |
Tier III |
| 4 |
NC_000006.12 |
6 |
6586577 |
- |
LY86-AS1 |
NNNGGCAGGGCCACAAACAT |
NGG |
2 |
0.3571 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000069.6 |
3 |
75592302 |
+ |
Serpini1 |
NNNTGCAGGGCCACAAACCT |
NGG |
2 |
0.3586 |
Tier II |
| 2 |
NC_000068.7 |
2 |
122685806 |
+ |
Slc30a4 |
NNNCGCAGGGCCTCAAACAT |
NGG |
2 |
0.2143 |
Tier II |
| 3 |
NC_000084.6 |
18 |
33922914 |
- |
Epb41l4a |
NNNCGCAGGGCCACAGACGT |
NGC |
2 |
0.0043 |
Tier II |
Other clones with same target sequence:
(none)
