Construct: sgRNA BRDN0001148156
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCTGCGTGAAGAGCATCGTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- SRPK3 (26576)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77397
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000023.11 |
X |
153782870 |
+ |
SRPK3 |
NNNGCGTGAAGAGCATCGTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000009.12 |
9 |
70601046 |
+ |
TRPM3 |
NNNGCCTGAGGAGCATCGTG |
NGG |
2 |
0.3788 |
Tier II |
| 3 |
NC_000008.11 |
8 |
18860953 |
- |
PSD3 |
NNNGTGTGAAGAGCATGGTG |
NGG |
2 |
0.0374 |
Tier II |
| 4 |
NC_000023.11 |
X |
153782870 |
+ |
LOC124905227 |
NNNGCGTGAAGAGCATCGTG |
NGG |
0 |
1.0 |
Tier III |
| 5 |
NC_000008.11 |
8 |
128549633 |
+ |
LINC00824 |
NNNGCGTGAAGAGCATTGTC |
NGG |
2 |
0.2 |
Tier III |
| 6 |
NC_000002.12 |
2 |
28449543 |
- |
LOC102723530 |
NNNGCGGGAAGAGCATCGTG |
NAG |
2 |
0.1525 |
Tier III |
| 7 |
NC_000008.11 |
8 |
18860953 |
- |
LOC124901897 |
NNNGTGTGAAGAGCATGGTG |
NGG |
2 |
0.0374 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
65683685 |
+ |
Scn2a |
NNNGCCTGAAGACCATCGTG |
NGG |
2 |
0.2871 |
Tier I |
| 2 |
NC_000068.7 |
2 |
66331495 |
- |
Scn1a |
NNNGCCTGAAGACCATCGTG |
NGG |
2 |
0.2871 |
Tier I |
| 3 |
NC_000071.6 |
5 |
100079765 |
+ |
Tmem150c |
NNNGTGTGAAAAGCATCGTG |
NGG |
2 |
0.6364 |
Tier II |
| 4 |
NC_000085.6 |
19 |
53969283 |
- |
Shoc2 |
NNNGCGTGAAGAGCATAATG |
NGG |
2 |
0.3231 |
Tier II |
| 5 |
NC_000077.6 |
11 |
110510927 |
- |
Map2k6 |
NNNGCGTGAAGAGCCTTGTG |
NGG |
2 |
0.1273 |
Tier II |
| 6 |
NC_000072.6 |
6 |
58831798 |
+ |
Herc3 |
NNNGCGTGAAGAGCATCGCG |
NCG |
2 |
0.0296 |
Tier II |
| 7 |
NC_000071.6 |
5 |
100079765 |
+ |
Tmem150cos |
NNNGTGTGAAAAGCATCGTG |
NGG |
2 |
0.6364 |
Tier III |
Other clones with same target sequence:
(none)
