Construct: sgRNA BRDN0001148174
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGGTGTCAAGACAATCACGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TRRAP (8295)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76538
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000007.14 |
7 |
98910387 |
+ |
TRRAP |
NNNTGTCAAGACAATCACGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000004.12 |
4 |
147996405 |
- |
ARHGAP10 |
NNNTGTTAAGACAATCACAT |
NGG |
2 |
0.5804 |
Tier II |
| 3 |
NC_000008.11 |
8 |
60833922 |
+ |
CHD7 |
NNNTGTCAAGACAATCATAT |
NGG |
2 |
0.4592 |
Tier II |
| 4 |
NC_000001.11 |
1 |
20335764 |
- |
VWA5B1 |
NNNTGTCAAGACAATGACGA |
NGG |
2 |
0.0865 |
Tier II |
| 5 |
NC_000009.12 |
9 |
112798043 |
- |
SNX30 |
NNNTGTCAAGACCACCACGT |
NGG |
2 |
0.0574 |
Tier II |
| 6 |
NC_000006.12 |
6 |
137706160 |
+ |
PTPN11P3 |
NNNTGTCATGACAATCACTT |
NGG |
2 |
0.4 |
Tier III |
| 7 |
NC_000003.12 |
3 |
107105639 |
+ |
PTPN11P1 |
NNNTGTCATGACAATCACCT |
NGG |
2 |
0.269 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
144791155 |
+ |
Trrap |
NNNTGTCAAGACCATCACAT |
NGG |
2 |
0.1504 |
Tier I |
| 2 |
NC_000082.6 |
16 |
15678747 |
+ |
Prkdc |
NNNTGTTAAGACAATCATGT |
NGG |
2 |
0.5223 |
Tier II |
| 3 |
NC_000073.6 |
7 |
35529920 |
- |
Tdrd12 |
NNNTGTCAAGACATTCACAT |
NGG |
2 |
0.381 |
Tier II |
| 4 |
NC_000070.6 |
4 |
100326244 |
+ |
Ror1 |
NNNGGTCATGACAATCACGT |
NGG |
2 |
0.375 |
Tier II |
| 5 |
NC_000071.6 |
5 |
5539936 |
+ |
Gtpbp10 |
NNNTGTCTAGCCAATCACGT |
NGG |
2 |
0.1714 |
Tier II |
| 6 |
NC_000078.6 |
12 |
91773737 |
+ |
Ston2 |
NNNTGTCAATACCATCACGT |
NGG |
2 |
0.1711 |
Tier II |
| 7 |
NC_000076.6 |
10 |
67017297 |
+ |
Reep3 |
NNNTGTCAAGAAAATGACGT |
NGG |
2 |
0.1099 |
Tier II |
| 8 |
NC_000068.7 |
2 |
166888136 |
+ |
Arfgef2 |
NNNTGTCAAGACCGTCACGT |
NGG |
2 |
0.0982 |
Tier II |
| 9 |
NC_000070.6 |
4 |
146553863 |
- |
Zfp993 |
NNNTGTCAAGCCAATCAGGT |
NGG |
2 |
0.0533 |
Tier II |
| 10 |
NC_000070.6 |
4 |
146736306 |
- |
Zfp993 |
NNNTGTCAAGCCAATCAGGT |
NGG |
2 |
0.0533 |
Tier II |
| 11 |
NC_000076.6 |
10 |
67017297 |
+ |
Gm31763 |
NNNTGTCAAGAAAATGACGT |
NGG |
2 |
0.1099 |
Tier III |
| 12 |
NC_000070.6 |
4 |
145718963 |
- |
Vmn2r-ps15 |
NNNTGTCAAGCCAATCAGGT |
NGG |
2 |
0.0533 |
Tier III |
| 13 |
NC_000070.6 |
4 |
146213411 |
- |
Vmn2r-ps17 |
NNNTGTCAAGCCAATCAGGT |
NGG |
2 |
0.0533 |
Tier III |
| 14 |
NC_000070.6 |
4 |
146213411 |
- |
Gm13165 |
NNNTGTCAAGCCAATCAGGT |
NGG |
2 |
0.0533 |
Tier III |
| 15 |
NC_000070.6 |
4 |
146553863 |
- |
Vmn2r-ps16 |
NNNTGTCAAGCCAATCAGGT |
NGG |
2 |
0.0533 |
Tier III |
Other clones with same target sequence:
(none)
