Construct: sgRNA BRDN0001148175
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGAGAGCACTGGAAATTCTG
Original Target:
- Taxon:
- Homo sapiens (human)
- Gene:
- MAP3K1 (4214)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000005.10 | 5 | 56875162 | + | MAP3K1 | NNNGAGCACTGGAAATTCTG | NGG | 0 | 1.0 | Tier I |
2 | NC_000016.10 | 16 | 20862169 | + | DCUN1D3 | NNNCAGCACTCGAAATTCTG | NGG | 2 | 0.2269 | Tier I |
3 | NC_000002.12 | 2 | 96290690 | - | SNRNP200 | NNNGAGCACTGGACATTCTG | NAG | 2 | 0.0556 | Tier I |
4 | NC_000017.11 | 17 | 51990248 | - | CA10 | NNNGAGTACTGGAAATTCTG | NGG | 1 | 0.8125 | Tier II |
5 | NC_000008.11 | 8 | 99418157 | - | VPS13B | NNNAAGAACTGGAAATTCTG | NGG | 2 | 0.675 | Tier II |
6 | NC_000010.11 | 10 | 62396110 | + | ZNF365 | NNNGAGCAGAGGAAATTCTG | NGG | 2 | 0.5306 | Tier II |
7 | NC_000009.12 | 9 | 69505425 | + | APBA1 | NNNGAGAACTGGAAATCCTG | NGG | 2 | 0.5294 | Tier II |
8 | NC_000021.9 | 21 | 28821693 | - | N6AMT1 | NNNGGGCACTGGAAATTCTT | NGG | 2 | 0.504 | Tier II |
9 | NC_000019.10 | 19 | 46174216 | - | IGFL2 | NNNGAGCCCTGAAAATTCTG | NGG | 2 | 0.4 | Tier II |
10 | NC_000004.12 | 4 | 148283378 | - | NR3C2 | NNNGAACACTGTAAATTCTG | NGG | 2 | 0.3846 | Tier II |
11 | NC_000004.12 | 4 | 113026613 | + | ANK2 | NNNGAGCATTGGTAATTCTG | NGG | 2 | 0.2625 | Tier II |
12 | NC_000009.12 | 9 | 120512768 | + | CDK5RAP2 | NNNGAGCAGTGTAAATTCTG | NGG | 2 | 0.2381 | Tier II |
13 | NC_000013.11 | 13 | 37019251 | - | SUPT20H | NNNCAGCACTTGAAATTCTG | NGG | 2 | 0.2036 | Tier II |
14 | NC_000005.10 | 5 | 179885703 | - | TBC1D9B | NNNGAGCACTGGAAATCCAG | NGG | 2 | 0.2017 | Tier II |
15 | NC_000013.11 | 13 | 32285806 | + | FRY | NNNTCGCACTGGAAATTCTG | NGG | 2 | 0.1818 | Tier II |
16 | NC_000011.10 | 11 | 48036352 | - | PTPRJ | NNNGAGCACTGGAAACTCTG | NAG | 2 | 0.1728 | Tier II |
17 | NC_000001.11 | 1 | 230932087 | + | TTC13 | NNNGAGCTCTTGAAATTCTG | NGG | 2 | 0.1648 | Tier II |
18 | NC_000004.12 | 4 | 165886191 | + | TLL1 | NNNGAGCACTGGATATTCTG | NAG | 2 | 0.1383 | Tier II |
19 | NC_000011.10 | 11 | 112146128 | + | IL18 | NNNGTGCACTGGAAAGTCTG | NGG | 2 | 0.1259 | Tier II |
20 | NC_000003.12 | 3 | 16933292 | + | PLCL2 | NNNGAGCACTGGAAATTGTA | NGG | 2 | 0.125 | Tier II |
21 | NC_000019.10 | 19 | 50660721 | + | SHANK1 | NNNGAGCACTGAAAATGCTG | NGG | 2 | 0.1098 | Tier II |
22 | NC_000012.12 | 12 | 109072655 | - | USP30 | NNNCAGCACTGGAAATGCTG | NGG | 2 | 0.0623 | Tier II |
23 | NC_000021.9 | 21 | 33676749 | + | ITSN1 | NNNGAGCACTGGGAATTCTG | NGA | 2 | 0.0453 | Tier II |
24 | NC_000009.12 | 9 | 131469964 | + | PRRC2B | NNNGAGCACTGGCCATTCTG | NGG | 2 | 0.0451 | Tier II |
25 | NC_000012.12 | 12 | 103125504 | + | C12orf42 | NNNGAGCACTGGAAAATCTG | NGC | 2 | 0.0202 | Tier II |
26 | NC_000011.10 | 11 | 17819626 | - | SERGEF | NNNGAGCACTGGAAATTCAG | NGA | 2 | 0.0198 | Tier II |
27 | NC_000021.9 | 21 | 21388613 | + | NCAM2 | NNNGAGCACTGGAAATGCTG | NCG | 2 | 0.0126 | Tier II |
28 | NC_000012.12 | 12 | 89474773 | + | POC1B | NNNGAGCACTGGAAGTTCTG | NGT | 2 | 0.0105 | Tier II |
29 | NC_000003.12 | 3 | 33210212 | + | SUSD5 | NNNGAGCACTGGAAATTCAG | NGT | 2 | 0.0046 | Tier II |
30 | NC_000023.11 | X | 22316262 | - | PTCHD1-AS | NNNGAGCACTGCAAATTCTG | NGG | 1 | 0.5294 | Tier III |
31 | NC_000004.12 | 4 | 13696001 | + | LINC01182 | NNNCAGCAATGGAAATTCTG | NGG | 2 | 0.4538 | Tier III |
32 | NC_000019.10 | 19 | 46174216 | - | LOC105372424 | NNNGAGCCCTGAAAATTCTG | NGG | 2 | 0.4 | Tier III |
33 | NC_000005.10 | 5 | 128678265 | - | LOC105379168 | NNNGAGCTCTGAAAATTCTG | NGG | 2 | 0.4 | Tier III |
34 | NC_000014.9 | 14 | 33961661 | - | LOC102724945 | NNNGGGCACTGGAAATACTG | NGG | 2 | 0.384 | Tier III |
35 | NC_000014.9 | 14 | 33961661 | - | LOC105370446 | NNNGGGCACTGGAAATACTG | NGG | 2 | 0.384 | Tier III |
36 | NC_000020.11 | 20 | 39132984 | - | LOC107985448 | NNNGAGCACTGGAGATCCTG | NGG | 2 | 0.3294 | Tier III |
37 | NC_000012.12 | 12 | 27192505 | - | LOC124902904 | NNNGAGCTCTGGAAACTCTG | NGG | 2 | 0.2857 | Tier III |
38 | NC_000003.12 | 3 | 70178441 | - | SAMMSON | NNNCAGCACTGGAAATTATG | NGG | 2 | 0.2851 | Tier III |
39 | NC_000004.12 | 4 | 113026613 | + | LOC124900761 | NNNGAGCATTGGTAATTCTG | NGG | 2 | 0.2625 | Tier III |
40 | NC_000013.11 | 13 | 31922482 | - | EEF1DP3 | NNNGAGCACTGGAAATTATG | NAG | 2 | 0.1396 | Tier III |
41 | NC_000023.11 | X | 2594584 | + | LINC03112 | NNNGAGCCCTGGAATTTCTG | NGG | 2 | 0.0857 | Tier III |
42 | NC_000024.10 | Y | 2594584 | + | LINC03112 | NNNGAGCCCTGGAATTTCTG | NGG | 2 | 0.0857 | Tier III |
43 | NC_000013.11 | 13 | 50435792 | - | DLEU1 | NNNGAGCACTGGAAATAGTG | NGG | 2 | 0.0711 | Tier III |
44 | NC_000001.11 | 1 | 19873581 | + | LOC105376823 | NNNGAGCACTGGAAATGCAG | NGG | 2 | 0.0336 | Tier III |
45 | NC_000007.14 | 7 | 17287703 | + | LOC101927609 | NNNGAGCACTGGAAATTTTG | NTG | 2 | 0.025 | Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000080.6 | 14 | 31162187 | + | Stab1 | NNNGAGCACTGGGAATTCTG | NGG | 1 | 0.6522 | Tier I |
2 | NC_000079.6 | 13 | 111760101 | - | Map3k1 | NNNGAGCACTGGAAACTCTG | NAG | 2 | 0.1728 | Tier I |
3 | NC_000075.6 | 9 | 21835746 | + | Angptl8 | NNNGAGCACTGGAATTCCTG | NGG | 2 | 0.1412 | Tier I |
4 | NC_000068.7 | 2 | 97293737 | - | Lrrc4c | NNNGAGCAATGGAAATTCTA | NGG | 2 | 0.8036 | Tier II |
5 | NC_000077.6 | 11 | 6039171 | + | Camk2b | NNNGAGAAATGGAAATTCTG | NGG | 2 | 0.6429 | Tier II |
6 | NC_000086.7 | X | 130337165 | + | Diaph2 | NNNGATCACTGGAAATTCTT | NGG | 2 | 0.4667 | Tier II |
7 | NC_000072.6 | 6 | 16884882 | + | Tfec | NNNAAGCACTCGAAATTCTG | NGG | 2 | 0.3857 | Tier II |
8 | NC_000083.6 | 17 | 56844976 | - | Acsbg2 | NNNGGGCTCTGGAAATTCTG | NGG | 2 | 0.3086 | Tier II |
9 | NC_000067.6 | 1 | 59524584 | + | Gm973 | NNNGAGCTCTGGAAATCCTG | NGG | 2 | 0.3025 | Tier II |
10 | NC_000069.6 | 3 | 41715972 | - | Sclt1 | NNNGAGCTCTGGGAATTCTG | NGG | 2 | 0.2795 | Tier II |
11 | NC_000084.6 | 18 | 5628644 | + | Zeb1 | NNNGAGCACTGGTAAATCTG | NGG | 2 | 0.2727 | Tier II |
12 | NC_000069.6 | 3 | 94762823 | - | Cgn | NNNGAGCTGTGGAAATTCTG | NGG | 2 | 0.2653 | Tier II |
13 | NC_000068.7 | 2 | 135334434 | + | Plcb1 | NNNGAGGACTGGATATTCTG | NGG | 2 | 0.251 | Tier II |
14 | NC_000079.6 | 13 | 54540625 | + | Simc1 | NNNTACCACTGGAAATTCTG | NGG | 2 | 0.2479 | Tier II |
15 | NC_000085.6 | 19 | 37270183 | + | Ide | NNNTAGCACTGGGAATTCTG | NGG | 2 | 0.2372 | Tier II |
16 | NC_000079.6 | 13 | 53279405 | - | Ror2 | NNNGGGCACTGGAACTTCTG | NGG | 2 | 0.1964 | Tier II |
17 | NC_000071.6 | 5 | 124756428 | - | Dnah10 | NNNCAGCACTGGAAAGTCTG | NGG | 2 | 0.1833 | Tier II |
18 | NC_000080.6 | 14 | 26876478 | + | Dnah12 | NNNCAGCACTGGAAAGTCTG | NGG | 2 | 0.1833 | Tier II |
19 | NC_000075.6 | 9 | 21835746 | + | Dock6 | NNNGAGCACTGGAATTCCTG | NGG | 2 | 0.1412 | Tier II |
20 | NC_000075.6 | 9 | 79772997 | - | Tmem30a | NNNCAGCACTGGAAATTCTG | NAG | 2 | 0.1373 | Tier II |
21 | NC_000084.6 | 18 | 53736856 | - | Cep120 | NNNGGGCACTGGAAATGCTG | NGG | 2 | 0.0847 | Tier II |
22 | NC_000073.6 | 7 | 111481723 | + | Galnt18 | NNNGAGCACTGGAAATTCAG | NAG | 2 | 0.0741 | Tier II |
23 | NC_000078.6 | 12 | 76437339 | + | Ppp1r36 | NNNGAGCACCGGAAATGCTG | NGG | 2 | 0.0627 | Tier II |
24 | NC_000069.6 | 3 | 68342592 | - | Schip1 | NNNGAGCACTGGAAATCCTG | NGA | 2 | 0.049 | Tier II |
25 | NC_000069.6 | 3 | 68342592 | - | Iqschfp | NNNGAGCACTGGAAATCCTG | NGA | 2 | 0.049 | Tier II |
26 | NC_000086.7 | X | 92477489 | + | Mageb18 | NNNGAGCACTGGAGATTCTG | NGA | 2 | 0.0324 | Tier II |
27 | NC_000070.6 | 4 | 6767685 | + | Tox | NNNGACCACTGGAAATTCTG | NTG | 2 | 0.0266 | Tier II |
28 | NC_000076.6 | 10 | 75675794 | - | Cabin1 | NNNGAGCACTGGACATGCTG | NGG | 2 | 0.0252 | Tier II |
29 | NC_000081.6 | 15 | 6470958 | + | C9 | NNNAAGCACTGGAAATTCTG | NGT | 2 | 0.0145 | Tier II |
30 | NC_000068.7 | 2 | 32697246 | + | Fpgs | NNNGAGCACTGGAAATTCTG | NCA | 2 | 0.0 | Tier II |
31 | NC_000081.6 | 15 | 51294102 | - | Gm19303 | NNNGAGCACTGGAAATCCTC | NGG | 2 | 0.3025 | Tier III |
32 | NC_000069.6 | 3 | 94663371 | - | BC021767 | NNNGAGCTGTGGAAATTCTG | NGG | 2 | 0.2653 | Tier III |
33 | NC_000079.6 | 13 | 48196363 | + | A330033J07Rik | NNNTAGCACTGGAAATTCTG | NGC | 2 | 0.0081 | Tier III |