Construct: sgRNA BRDN0001148175

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GGAGAGCACTGGAAATTCTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAP3K1 (4214)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77826

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000005.10 5 56875162 + MAP3K1 NNNGAGCACTGGAAATTCTG NGG 0 1.0 Tier I
2 NC_000016.10 16 20862169 + DCUN1D3 NNNCAGCACTCGAAATTCTG NGG 2 0.2269 Tier I
3 NC_000002.12 2 96290690 - SNRNP200 NNNGAGCACTGGACATTCTG NAG 2 0.0556 Tier I
4 NC_000017.11 17 51990248 - CA10 NNNGAGTACTGGAAATTCTG NGG 1 0.8125 Tier II
5 NC_000008.11 8 99418157 - VPS13B NNNAAGAACTGGAAATTCTG NGG 2 0.675 Tier II
6 NC_000010.11 10 62396110 + ZNF365 NNNGAGCAGAGGAAATTCTG NGG 2 0.5306 Tier II
7 NC_000009.12 9 69505425 + APBA1 NNNGAGAACTGGAAATCCTG NGG 2 0.5294 Tier II
8 NC_000021.9 21 28821693 - N6AMT1 NNNGGGCACTGGAAATTCTT NGG 2 0.504 Tier II
9 NC_000019.10 19 46174216 - IGFL2 NNNGAGCCCTGAAAATTCTG NGG 2 0.4 Tier II
10 NC_000004.12 4 148283378 - NR3C2 NNNGAACACTGTAAATTCTG NGG 2 0.3846 Tier II
11 NC_000004.12 4 113026613 + ANK2 NNNGAGCATTGGTAATTCTG NGG 2 0.2625 Tier II
12 NC_000009.12 9 120512768 + CDK5RAP2 NNNGAGCAGTGTAAATTCTG NGG 2 0.2381 Tier II
13 NC_000013.11 13 37019251 - SUPT20H NNNCAGCACTTGAAATTCTG NGG 2 0.2036 Tier II
14 NC_000005.10 5 179885703 - TBC1D9B NNNGAGCACTGGAAATCCAG NGG 2 0.2017 Tier II
15 NC_000013.11 13 32285806 + FRY NNNTCGCACTGGAAATTCTG NGG 2 0.1818 Tier II
16 NC_000011.10 11 48036352 - PTPRJ NNNGAGCACTGGAAACTCTG NAG 2 0.1728 Tier II
17 NC_000001.11 1 230932087 + TTC13 NNNGAGCTCTTGAAATTCTG NGG 2 0.1648 Tier II
18 NC_000004.12 4 165886191 + TLL1 NNNGAGCACTGGATATTCTG NAG 2 0.1383 Tier II
19 NC_000011.10 11 112146128 + IL18 NNNGTGCACTGGAAAGTCTG NGG 2 0.1259 Tier II
20 NC_000003.12 3 16933292 + PLCL2 NNNGAGCACTGGAAATTGTA NGG 2 0.125 Tier II
21 NC_000019.10 19 50660721 + SHANK1 NNNGAGCACTGAAAATGCTG NGG 2 0.1098 Tier II
22 NC_000012.12 12 109072655 - USP30 NNNCAGCACTGGAAATGCTG NGG 2 0.0623 Tier II
23 NC_000021.9 21 33676749 + ITSN1 NNNGAGCACTGGGAATTCTG NGA 2 0.0453 Tier II
24 NC_000009.12 9 131469964 + PRRC2B NNNGAGCACTGGCCATTCTG NGG 2 0.0451 Tier II
25 NC_000012.12 12 103125504 + C12orf42 NNNGAGCACTGGAAAATCTG NGC 2 0.0202 Tier II
26 NC_000011.10 11 17819626 - SERGEF NNNGAGCACTGGAAATTCAG NGA 2 0.0198 Tier II
27 NC_000021.9 21 21388613 + NCAM2 NNNGAGCACTGGAAATGCTG NCG 2 0.0126 Tier II
28 NC_000012.12 12 89474773 + POC1B NNNGAGCACTGGAAGTTCTG NGT 2 0.0105 Tier II
29 NC_000003.12 3 33210212 + SUSD5 NNNGAGCACTGGAAATTCAG NGT 2 0.0046 Tier II
30 NC_000023.11 X 22316262 - PTCHD1-AS NNNGAGCACTGCAAATTCTG NGG 1 0.5294 Tier III
31 NC_000004.12 4 13696001 + LINC01182 NNNCAGCAATGGAAATTCTG NGG 2 0.4538 Tier III
32 NC_000019.10 19 46174216 - LOC105372424 NNNGAGCCCTGAAAATTCTG NGG 2 0.4 Tier III
33 NC_000005.10 5 128678265 - LOC105379168 NNNGAGCTCTGAAAATTCTG NGG 2 0.4 Tier III
34 NC_000014.9 14 33961661 - LOC102724945 NNNGGGCACTGGAAATACTG NGG 2 0.384 Tier III
35 NC_000014.9 14 33961661 - LOC105370446 NNNGGGCACTGGAAATACTG NGG 2 0.384 Tier III
36 NC_000020.11 20 39132984 - LOC107985448 NNNGAGCACTGGAGATCCTG NGG 2 0.3294 Tier III
37 NC_000012.12 12 27192505 - LOC124902904 NNNGAGCTCTGGAAACTCTG NGG 2 0.2857 Tier III
38 NC_000003.12 3 70178441 - SAMMSON NNNCAGCACTGGAAATTATG NGG 2 0.2851 Tier III
39 NC_000004.12 4 113026613 + LOC124900761 NNNGAGCATTGGTAATTCTG NGG 2 0.2625 Tier III
40 NC_000013.11 13 31922482 - EEF1DP3 NNNGAGCACTGGAAATTATG NAG 2 0.1396 Tier III
41 NC_000023.11 X 2594584 + LINC03112 NNNGAGCCCTGGAATTTCTG NGG 2 0.0857 Tier III
42 NC_000024.10 Y 2594584 + LINC03112 NNNGAGCCCTGGAATTTCTG NGG 2 0.0857 Tier III
43 NC_000013.11 13 50435792 - DLEU1 NNNGAGCACTGGAAATAGTG NGG 2 0.0711 Tier III
44 NC_000001.11 1 19873581 + LOC105376823 NNNGAGCACTGGAAATGCAG NGG 2 0.0336 Tier III
45 NC_000007.14 7 17287703 + LOC101927609 NNNGAGCACTGGAAATTTTG NTG 2 0.025 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000080.6 14 31162187 + Stab1 NNNGAGCACTGGGAATTCTG NGG 1 0.6522 Tier I
2 NC_000079.6 13 111760101 - Map3k1 NNNGAGCACTGGAAACTCTG NAG 2 0.1728 Tier I
3 NC_000075.6 9 21835746 + Angptl8 NNNGAGCACTGGAATTCCTG NGG 2 0.1412 Tier I
4 NC_000068.7 2 97293737 - Lrrc4c NNNGAGCAATGGAAATTCTA NGG 2 0.8036 Tier II
5 NC_000077.6 11 6039171 + Camk2b NNNGAGAAATGGAAATTCTG NGG 2 0.6429 Tier II
6 NC_000086.7 X 130337165 + Diaph2 NNNGATCACTGGAAATTCTT NGG 2 0.4667 Tier II
7 NC_000072.6 6 16884882 + Tfec NNNAAGCACTCGAAATTCTG NGG 2 0.3857 Tier II
8 NC_000083.6 17 56844976 - Acsbg2 NNNGGGCTCTGGAAATTCTG NGG 2 0.3086 Tier II
9 NC_000067.6 1 59524584 + Gm973 NNNGAGCTCTGGAAATCCTG NGG 2 0.3025 Tier II
10 NC_000069.6 3 41715972 - Sclt1 NNNGAGCTCTGGGAATTCTG NGG 2 0.2795 Tier II
11 NC_000084.6 18 5628644 + Zeb1 NNNGAGCACTGGTAAATCTG NGG 2 0.2727 Tier II
12 NC_000069.6 3 94762823 - Cgn NNNGAGCTGTGGAAATTCTG NGG 2 0.2653 Tier II
13 NC_000068.7 2 135334434 + Plcb1 NNNGAGGACTGGATATTCTG NGG 2 0.251 Tier II
14 NC_000079.6 13 54540625 + Simc1 NNNTACCACTGGAAATTCTG NGG 2 0.2479 Tier II
15 NC_000085.6 19 37270183 + Ide NNNTAGCACTGGGAATTCTG NGG 2 0.2372 Tier II
16 NC_000079.6 13 53279405 - Ror2 NNNGGGCACTGGAACTTCTG NGG 2 0.1964 Tier II
17 NC_000071.6 5 124756428 - Dnah10 NNNCAGCACTGGAAAGTCTG NGG 2 0.1833 Tier II
18 NC_000080.6 14 26876478 + Dnah12 NNNCAGCACTGGAAAGTCTG NGG 2 0.1833 Tier II
19 NC_000075.6 9 21835746 + Dock6 NNNGAGCACTGGAATTCCTG NGG 2 0.1412 Tier II
20 NC_000075.6 9 79772997 - Tmem30a NNNCAGCACTGGAAATTCTG NAG 2 0.1373 Tier II
21 NC_000084.6 18 53736856 - Cep120 NNNGGGCACTGGAAATGCTG NGG 2 0.0847 Tier II
22 NC_000073.6 7 111481723 + Galnt18 NNNGAGCACTGGAAATTCAG NAG 2 0.0741 Tier II
23 NC_000078.6 12 76437339 + Ppp1r36 NNNGAGCACCGGAAATGCTG NGG 2 0.0627 Tier II
24 NC_000069.6 3 68342592 - Schip1 NNNGAGCACTGGAAATCCTG NGA 2 0.049 Tier II
25 NC_000069.6 3 68342592 - Iqschfp NNNGAGCACTGGAAATCCTG NGA 2 0.049 Tier II
26 NC_000086.7 X 92477489 + Mageb18 NNNGAGCACTGGAGATTCTG NGA 2 0.0324 Tier II
27 NC_000070.6 4 6767685 + Tox NNNGACCACTGGAAATTCTG NTG 2 0.0266 Tier II
28 NC_000076.6 10 75675794 - Cabin1 NNNGAGCACTGGACATGCTG NGG 2 0.0252 Tier II
29 NC_000081.6 15 6470958 + C9 NNNAAGCACTGGAAATTCTG NGT 2 0.0145 Tier II
30 NC_000068.7 2 32697246 + Fpgs NNNGAGCACTGGAAATTCTG NCA 2 0.0 Tier II
31 NC_000081.6 15 51294102 - Gm19303 NNNGAGCACTGGAAATCCTC NGG 2 0.3025 Tier III
32 NC_000069.6 3 94663371 - BC021767 NNNGAGCTGTGGAAATTCTG NGG 2 0.2653 Tier III
33 NC_000079.6 13 48196363 + A330033J07Rik NNNTAGCACTGGAAATTCTG NGC 2 0.0081 Tier III
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Other clones with same target sequence:

(none)