Construct: sgRNA BRDN0001148177

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CAGGAATACCACCAAATCGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AK5 (26289)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76553

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000001.11 1 77340390 - AK5 NNNGAATACCACCAAATCGG NGG 0 1.0 Tier I
2 NC_000001.11 1 205925488 + SLC26A9 NNNGAATACCACCATAGCGG NGG 2 0.0235 Tier II
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000075.6 9 21419302 - Qtrt1 NNNGCAGACCACCAAATCGG NGG 2 0.2941 Tier I
2 NC_000079.6 13 8438515 + Adarb2 NNNGAATACCACCAATTCGG NTG 2 0.0 Tier II
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Other clones with same target sequence:

(none)