Construct: sgRNA BRDN0001148179
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ACATGATGTAGAGTTGCTCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PLXNA4 (91584)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76881
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000007.14 |
7 |
132298119 |
+ |
PLXNA4 |
NNNTGATGTAGAGTTGCTCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000017.11 |
17 |
40454002 |
- |
IGFBP4 |
NNNTGATGTAGAGGTCCTCG |
NGG |
2 |
0.0 |
Tier I |
| 3 |
NC_000020.11 |
20 |
43923766 |
+ |
TOX2 |
NNNAGATGGAGAGTTGCTCG |
NGG |
2 |
0.2948 |
Tier II |
| 4 |
NC_000007.14 |
7 |
28053889 |
- |
JAZF1 |
NNNTGATGCAGAGCTGCTCG |
NGG |
2 |
0.2637 |
Tier II |
| 5 |
NC_000004.12 |
4 |
31072589 |
+ |
PCDH7 |
NNNTCATGTAGAGTTGCTGG |
NGG |
2 |
0.0982 |
Tier II |
| 6 |
NC_000013.11 |
13 |
44809563 |
+ |
LINC00330 |
NNNTGAGGTAAAGTTGCTCG |
NGG |
2 |
0.5882 |
Tier III |
| 7 |
NC_000007.14 |
7 |
28053889 |
- |
LOC105375208 |
NNNTGATGCAGAGCTGCTCG |
NGG |
2 |
0.2637 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000072.6 |
6 |
32310489 |
+ |
Plxna4 |
NNNTGATGTAGAGTTGCTCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000082.6 |
16 |
46395133 |
+ |
Nectin3 |
NNNTGATGTAGAGTCTCTCG |
NGG |
2 |
0.0 |
Tier I |
| 3 |
NC_000074.6 |
8 |
91516473 |
+ |
Fto |
NNNTGGGGTAGAGTTGCTCG |
NGG |
2 |
0.4202 |
Tier II |
| 4 |
NC_000084.6 |
18 |
10726878 |
+ |
Mib1 |
NNNTGGTGTGGAGTTGCTCG |
NGG |
2 |
0.3968 |
Tier II |
| 5 |
NC_000077.6 |
11 |
107100085 |
- |
Bptf |
NNNTGCTGTAGAGTTGCTTG |
NGG |
2 |
0.2098 |
Tier II |
| 6 |
NC_000070.6 |
4 |
89368792 |
+ |
Gm12610 |
NNNTGATGCAGAGTTGCTTG |
NGG |
2 |
0.426 |
Tier III |
| 7 |
NC_000080.6 |
14 |
15055693 |
- |
Gm31517 |
NNNTGATGTTGAGTTGCTGG |
NGG |
2 |
0.1103 |
Tier III |
Other clones with same target sequence:
(none)
