Construct: sgRNA BRDN0001148181

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CTTACTTACAGTGAATTCCC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PAK3 (5063)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75679

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000023.11 X 111142189 - PAK3 NNNACTTACAGTGAATTCCC NGG 0 1.0 Tier I
2 NC_000009.12 9 92079469 - SPTLC1 NNNAAATACAGTGAATTCCC NGG 2 0.4952 Tier I
3 NC_000003.12 3 196802020 - PAK2 NNNACTTACAGTGAATTCTC NAG 2 0.1197 Tier I
4 NC_000001.11 1 26276753 - CEP85 NNNACTCACAGTGAATGCCC NGG 2 0.0809 Tier I
5 NC_000017.11 17 35083143 + RFFL NNNACATACAGAGAATTCCC NGG 2 0.6933 Tier II
6 NC_000006.12 6 147387219 - STXBP5 NNNAATTACAGTAAATTCCC NGG 2 0.5275 Tier II
7 NC_000005.10 5 159946245 + ADRA1B NNNATTTACAGAGAATTCCC NGG 2 0.5091 Tier II
8 NC_000001.11 1 237154124 + RYR2 NNNACTTCCAGTGAAATCCC NGG 2 0.3896 Tier II
9 NC_000003.12 3 29423035 - RBMS3 NNNTCTTACAGTGAATTACC NGG 2 0.3427 Tier II
10 NC_000002.12 2 74129786 + TET3 NNNACTCACACTGAATTCCC NGG 2 0.2946 Tier II
11 NC_000004.12 4 86257401 + MAPK10 NNNACTCACAGTGAATTCCC NAG 2 0.1782 Tier II
12 NC_000013.11 13 110657904 - CARS2 NNNACTCACAGTGCATTCCC NGG 2 0.1473 Tier II
13 NC_000015.10 15 101031145 + LRRK1 NNNACTTACAGTGAAATGCC NGG 2 0.1212 Tier II
14 NC_000016.10 16 75672381 - CPHXL2 NNNTCTTACAGTGAATGCCC NGG 2 0.0749 Tier II
15 NC_000003.12 3 173045131 + SPATA16 NNNACTTACAGTAAATTCCC NTG 2 0.036 Tier II
16 NC_000009.12 9 121479074 + GGTA1 NNNACTTACAGTGAAGTCCC NGA 2 0.024 Tier II
17 NC_000005.10 5 168808201 + SLIT3 NNNACTTACACTGAATTCCC NGC 2 0.0095 Tier II
18 NC_000012.12 12 116817673 - RNFT2 NNNACTTACAGTGAAGTCCC NGT 2 0.0056 Tier II
19 NC_000005.10 5 142797092 - ARHGAP26 NNNACTTACAGTGACTTCCC NGT 2 0.0044 Tier II
20 NC_000017.11 17 35083143 + RAD51L3-RFFL NNNACATACAGAGAATTCCC NGG 2 0.6933 Tier III
21 NC_000005.10 5 66434148 - LOC105379003 NNNACTTACAGTGAATTTCT NGG 2 0.1929 Tier III
22 NC_000004.12 4 151918596 - LOC127898556 NNNCCTTACAGTGAATTCCC NGC 2 0.0078 Tier III
23 NC_000004.12 4 151918596 - LOC127898557 NNNCCTTACAGTGAATTCCC NGC 2 0.0078 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000086.7 X 143709741 - Pak3 NNNACTTACAGTGAATTCCC NGG 0 1.0 Tier I
2 NC_000080.6 14 51996126 - Arhgef40 NNNACTGACAGTGAGTTCCC NGG 2 0.3824 Tier I
3 NC_000076.6 10 30704609 + Ncoa7 NNNACTTACAGTGTATTCCA NGG 2 0.2667 Tier I
4 NC_000071.6 5 76557310 - Exoc1 NNNACATACAGTGAATTCCC NGG 1 0.8667 Tier II
5 NC_000086.7 X 102329145 + Hdac8 NNNACTTAAAGAGAATTCCC NGG 2 0.6857 Tier II
6 NC_000070.6 4 139277026 - Capzb NNNACCTACAGTGAACTCCC NGG 2 0.6061 Tier II
7 NC_000073.6 7 55125177 - Luzp2 NNNACTCACAGTGAATTTCC NGG 2 0.442 Tier II
8 NC_000067.6 1 146575257 + Brinp3 NNNTCTCACAGTGAATTCCC NGG 2 0.4375 Tier II
9 NC_000068.7 2 18184453 + Mllt10 NNNACATACAGTGAATTCCA NGG 2 0.4333 Tier II
10 NC_000067.6 1 181104887 + Nvl NNNACTTGCAGTGGATTCCC NGG 2 0.3422 Tier II
11 NC_000079.6 13 58923580 - Ntrk2 NNNAGTTACAGTGTATTCCC NGG 2 0.32 Tier II
12 NC_000075.6 9 26799697 - Glb1l2 NNNTCTTACAGTGAATTCTC NGG 2 0.2937 Tier II
13 NC_000077.6 11 46162660 + Nipal4 NNNGCTTTCAGTGAATTCCC NGG 2 0.2679 Tier II
14 NC_000067.6 1 88425088 + Spp2 NNNACATACAGTGACTTCCC NGG 2 0.2364 Tier II
15 NC_000077.6 11 37026919 + Tenm2 NNNACCTACAGTGATTTCCC NGG 2 0.1818 Tier II
16 NC_000070.6 4 68849310 - Brinp1 NNNACTTACAGTGAATTCCC NGT 1 0.0161 Tier II
17 NC_000075.6 9 46455362 - Gm31432 NNNACTTACTGTAAATTCCC NGG 2 0.8145 Tier III
18 NC_000086.7 X 102329145 + Gm32262 NNNACTTAAAGAGAATTCCC NGG 2 0.6857 Tier III
19 NC_000067.6 1 72805941 + Gm30847 NNNACCTACAGTGACTTCCC NGG 2 0.2479 Tier III
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Other clones with same target sequence:

(none)